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Research Article| Volume 1, ISSUE 2, P329-343, June 1981

Detection of Free Bilirubin and Estimation of Reserve Albumin Binding Capacity

DOI:https://doi.org/10.1016/S0272-2712(18)31105-3
Detection of Free Bilirubin and Estimation of Reserve Albumin Binding Capacity
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      Detection of free bilirubin or decreased reserve albumin binding capacity (RABC), followed by appropriate clinical management, may help to prevent kernicterus in and improve survival of the immature neonate. The attributes and pitfalls of procedures that have been developed to determine RABC are highlighted; a modified procedure that is practical, simple, and rapid is recommended.
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      References

        • Alayoff A.
        • Kapitulnik J.
        • Konijn A.
        • et al.
        Bilirubin binding capacity of albumin isolated from cord-blood serum is less than that from serum of adults.
        Clin. Chem. 1980; 26: 738
        • Andrew G.
        • Chan G.
        • Schiff D.
        Lipid metabolism in the neonate. II. The effect of Intralipid on bilirubin binding in vitro and in vivo.
        J. Pediatr. 1976; 88: 279
        • Blondheim S.H.
        The relationship between the albumin concentration of serum and its dye-binding capacity.
        J. Lab. Clin. Med. 1955; 45: 740
        • Blondheim S.H.
        • Kapitulnik J.
        • Valaes T.
        • et al.
        Use of a Sephadex column to evaluate the bilirubin-binding capacity of the serum of infants with neonatal jaundice.
        Israel J. Med. Sci. 1972; 8: 22
        • Bratlid D.
        Bilirubin binding by human erythrocytes.
        Scand. J. Clin. Lab. Invest. 1972; 29: 91
        • Bratlid D.
        The effect of pH on bilirubin binding by human erythrocytes.
        Scand. J. Clin. Lab. Invest. 1972; 29: 453
        • Bratlid D.
        Reserve albumin binding capacity, salicylate saturation index, and red cell binding of bilirubin in neonatal jaundice.
        Arch. Dis. Child. 1973; 48: 393
        • Brodersen R.
        Determination of the vacant amount of high-affinity bilirubin binding site on serum albumin.
        Acta Pharmacol. Toxicol. 1978; 42: 153
        • Brodersen R.
        Bilirubin transport in the newborn infant, reviewed with relation to kernicterus.
        J. Pediatr. 1980; 96: 349
        • Brodersen R.
        • Bartels P.
        Enzymatic oxidation of bilirubin.
        Eur. J. Biochem. 1969; 10: 468
        • Brown A.K.
        • Eisinger J.
        • Blumberg W.E.
        • et al.
        A rapid fluorometric method for determining bilirubin levels and binding in the blood of neonates: Comparisons with a diazo method and with 2-(4′-hydroxybenzene) azobenzoic acid dye binding.
        Pediatrics. 1980; 65: 767
        • Cashore W.J.
        Free bilirubin concentrations and bilirubin-binding affinity in term and preterm infants.
        J. Pediatr. 1980; 96: 521
        • Cashore W.J.
        • Oh W.
        • Blumberg W.E.
        • et al.
        Rapid fluorometric assay of bilirubin and bilirubin binding capacity in blood of jaundiced neonates: Comparisons with other methods.
        Pediatrics. 1980; 66: 411
        • Free H.M.
        Estimation of bilirubin binding capacity of neonatal serum.
        Ann. Clin. Lab. Sci. 1978; 8: 64
        • Hertz H.
        A direct spectrophotometric method for determination of the concentration of available bilirubin binding sites in serum using bromphenol blue.
        Scand. J. Clin. Lab. Invest. 1975; 35: 545
        • Hertz H.
        Available bilirubin binding sites of serum from newborns determined by a direct spectrometric method using bromphenol blue.
        Scand. J. Clin. Lab. Invest. 1975; 35: 561
        • Howorth P.J.N.
        Determination of serum albumin in neonatal jaundice. The albumin saturation index.
        Clin. Chim. Acta. 1971; 32: 271
        • Hummel J.P.
        • Dreyer W.J.
        Measurement of protein-binding phenomena by gel filtration (preliminary notes).
        Biochim. Biophys. Acta. 1962; 63: 530
        • Hsia J.C.
        • Kwan N.H.
        • Er S.S.
        • et al.
        Development of a spin assay for reserve bilirubin loading capacity of human serum.
        Proc. Natl. Acad. Sci. USA. 1978; 75: 1542
        • Jacobsen J.
        Binding of bilirubin to human serum albumin — Determination of the dissociation constants.
        Fed. Eur. Biochem. Soc. Lett. 1969; 5: 112
        • Jacobsen J.
        • Wennberg R.P.
        Determination of unbound bilirubin in the serum of newborns.
        Clin. Chem. 1974; 20: 783
        • Jirsova V.
        • Jirsa M.
        • Heringova A.
        • et al.
        The use and possible diagnostic significance of Sephadex gel filtration of serum from icteric newborn.
        Biol. Neonat. 1967; ii: 204
        • Johnson L.
        • Boggs Jr., T.R.
        Bilirubin-dependent brain damage: Incidence and indications for treatment.
        in: Odell G.B. Shaffer R. Simopoulos A.P. Phototherapy in the Newborn: An Overview. National Academy of Sciences, Washington, D.C.1974
        • Kamisaka K.
        • Listowsky I.
        • Betheil J.J.
        • et al.
        Competitive binding of bilirubin, sulfobromophthalein, indocyanine green and other organic anions to human and bovine serum albumin.
        Biochim. Biophys. Acta. 1974; 365: 169
        • Kaufmann N.A.
        • Kapitulnik J.
        • Blondheim S.H.
        The absorption of bilirubin by Sephadex and its relationship to the criteria for exchange transfusion.
        Pediatrics. 1969; 44: 543
        • Kaufmann N.A.
        • Simcha A.J.
        • Blondheim S.H.
        The uptake of bilirubin by blood cells from plasma and its relationship to the criteria for exchange transfusion.
        Clin. Sci. 1967; 33: 201
        • Knight J.A.
        • Haymond R.E.
        The routine use of Sephadex to estimate “free” bilirubin and the residual serum bilirubin binding capacity (letter).
        Clin. Chem. 1977; 23: 1504
        • Kopelman A.E.
        • Brown R.S.
        • Odell G.B.
        The “bronze baby”, a complication of phototherapy.
        Pediatr. Res. 1971; 5: 638
        • Krasner J.
        Fluorescent properties of bovine serum albumin-bilirubin complex.
        Biochem. Med. 1973; 7: 135
        • Krasner J.
        • Giacoia G.P.
        • Yaffe S.J.
        Drug-protein binding in the newborn infant.
        Ann. N. Y. Acad. Sci. 1973; 226: 101
        • Lamola A.A.
        • Eisinger J.
        • Blumberg W.E.
        • et al.
        Fluorometric study of the partition of bilirubin among blood components: Basis for rapid microassays of bilirubin and bilirubin binding capacity in whole blood.
        Anal. Biochem. 1979; 100: 25
        • Lee K.-S.
        • Gartner L.M.
        Bilirubin binding by plasma proteins: A critical evaluation of methods and clinical implications.
        in: Scarpelli E.M. Cosmi E.V. Reviews in Perinatal Medicine. Raven Press, New York1978
        • Lee K.-S.
        • Gartner L.M.
        • Vaisman S.L.
        Measurement of bilirubin-albumin binding. I. Comparative analysis of four methods and four human serum albumin preparations.
        Pediatr. Res. 1978; 12: 301
        • Lee K.-S.
        • Gartner L.M.
        • Zarafu I.
        Fluorescent dye method for determination of the bilirubin-binding capacity of serum albumin.
        J. Pediatr. 1975; 86: 280
        • Lucey J.F.
        • Valaes T.
        • Doxiadis S.A.
        Serum albumin reserve PSP dye binding capacity in infants with kernicterus.
        Pediatrics. 1967; 39: 876
        • McCluskey S.B.
        • Storey G.N.B.
        • Brown G.K.
        • et al.
        Fluorometric determination of “albumin-titratable bilirubin” in the jaundiced neonate.
        Clin. Chem. 1975; 21: 1638
        • Odell G.B.
        Studies in kernicterus. I. The protein binding of bilirubin.
        J. Clin. Invest. 1959; 38: 823
        • Odell G.B.
        The dissociation of bilirubin from albumin and its clinical implications.
        J. Pediatr. 1959; 55: 268
        • Odell G.B.
        Influence of pH on the distribution of bilirubin between albumin and mitochon dria.
        Proc. Soc. Exp. Biol. Med. 1965; 120: 352
        • Odell G.B.
        Discussion of paper by Watson and Porter.
        J. Pediatr. 1965; 67: 1042
        • Odell G.B.
        The distribution of bilirubin between albumin and mitochondria.
        J. Pediatr. 1966; 68: 164
        • Odell G.B.
        Neonatal Hyperbilirubinemia. Grune and Stratton, New York1980
        • Odell G.B.
        • Cohen S.N.
        • Kelly P.C.
        Studies in kernicterus. II. The determination of the saturation of serum albumin with bilirubin.
        J. Pediatr. 1969; 74: 214
        • Odell G.B.
        • Storey G.N.B.
        • Rosenberg L.A.
        Studies in kernicterus. III. The saturation of serum proteins with bilirubin during neonatal life and its relationship to brain damage at five years.
        J. Pediatr. 1970; 76: 12
        • O’Sullivan W.J.
        • Brown G.K.
        • Storey G.N.B.
        Response to letter to editor.
        Clin. Chem. 1976; 22: 561
        • Porath J.
        • Flodin P.
        Gel filtration: A method for desalting and group separation.
        Nature. 1959; 283: 1657
        • Porter E.G.
        • Walters W.J.
        A rapid micromethod for measuring the reserve albumin binding capacity in serum from newborn infants with hyperbilirubinemia.
        J. Lab. Clin. Med. 1966; 67: 660
        • Rutkowski R.B.
        Bilirubin binding by primary sites of human serum albumin, with suppression of secondary-site interference by use of a high salt concentration.
        Clin. Chem. 1980; 26: 107
        • Sakamoto M.
        • Neubauer T.
        • Alpers J.B.
        Estimation of reserve bilirubin binding capacity (RBBC) of human serum (abstract).
        Clin. Chem. 1976; 22: 1187
        • Scheidt P.C.
        • Mellitis E.D.
        • Hardy J.B.
        • et al.
        Toxicity to bilirubin in neonates: Infant development during first year in relation to maximum neonatal serum bilirubin concentration.
        J. Pediatr. 1977; 91: 292
        • Schiff D.
        • Chan G.
        • Stern L.
        Sephadex G-25 quantitative estimation of free bilirubin potential in jaundiced newborn infants’ sera: A guide to the prevention of kernicterus.
        J. Lab. Clin. Med. 1972; 80: 455
        • Schmid R.
        • Diamond I.
        • Hammaker L.
        • et al.
        Interaction of bilirubin with albumin.
        Nature. 1965; 206: 1041
        • Waters W.J.
        • Porter E.G.
        Dye-binding capacity of serum albumin in hemolytic disease of the newborn.
        Am. J. Dis. Child. 1961; 102: 59
        • Waters W.J.
        • Porter E.
        Indications for exchange transfusion based upon the role of albumin in the treatment of hemolytic disease of the newborn.
        Pediatrics. 1964; 33: 749
        • Watson D.
        The absorption of bilirubin by erythrocytes.
        Clin. Chim. Acta. 1962; 7: 733
        • Wennberg R.P.
        • Ahlfors C.E.
        • Rasmussen L.F.
        Critical review — The pathochemistry of kernicterus.
        Early Hum. Dev. 1979; 3/4: 353
        • Wennberg R.P.
        • Rasmussen L.F.
        • Ahlfors C.E.
        • et al.
        Mechanized determination of the apparent unbound unconjugated bilirubin concentration in serum.
        Clin. Chem. 1979; 25: 1444

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      DOI: https://doi.org/10.1016/S0272-2712(18)31105-3

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      © 1981 Elsevier Inc. Published by Elsevier Inc. All rights reserved.

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