Research Article| Volume 4, ISSUE 4, P817-833, December 1984

Receptor Measurements

  • James H. McBride
    Corresponding author: Department of Pathology, UCLA Medical Center, Los Angeles, California 90024
    Assistant Head of Clinical Chemistry, Assistant Professor of Pathology, Department of Pathology, UCLA Medical Center, Los Angeles, California
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      Estrogen and progesterone receptor assays supply information that aids the physician in making informed judgments as to which patients are likely to respond to endocrine therapy. From the clinical laboratory viewpoint, the current receptor assays have been a success, but much remains to be done. Sensitivity, turnaround time, and cost will be greatly improved by new developments in the area of steroid receptor analysis, especially with the use of HPLC, IRA, and of monoclonal antibodies. The use of nuclear thyroid receptors has yet to be fully exploited, but this undoubtedly will happen. Although a wide variety of both bioassays and binding assays have been used to confirm Graves’ disease, it appears that the use of radioreceptor and ELISA assays for thyrotropin receptor antibody will become more popular.
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        • Bernal J.
        • Refetoff S.
        • Degroot C.J.
        Abnormalities of tri-iodothyronine binding to lymphocyte and fibroblast nuclei from a patient with peripheral tissue resistance to thyroid hormone action.
        J. Clin. Endocrinol. Metab. 1978; 47: 1266-1272
        • Blecher M.
        Receptors, antibodies and disease.
        Clin. Chem. 1984; 30: 1137-1156
        • Castaneda E.
        • Liao S.
        The use of anti-steroid antibodies in the characterization of steroid receptors.
        J. Biol. Chem. 1975; 250: 883-888
        • Chamness G.C.
        • McGuire W.L.
        Methods for analyzing steroid receptors in human breast cancer.
        in: McGuire W.L. Breast Cancer. Ed. 3. Advances in Research and Treatment. Plenum Press, New York1979: 149-197
        • Chamness G.C.
        • McGuire W.L.
        Estrogen/progesterone receptor assay in breast cancer: Problems and prospects.
        The Ligand Review. 1980; 2: 40-43
        • DeGroot C.J.
        • Refetoff S.
        • Strausser J.
        • et al.
        Nuclear triiodothyronine binding protein: Partial characterization and binding to chromatin.
        Pro. Natl. Acad. Sci. U.S.A. 1974; 71: 4042-4046
        • Eberhardt N.L.
        • Ring J.C.
        • lohnston C.K.
        • et al.
        Regulation of activity of chromatin receptors for thyroid hormone: Possible involvement of histone-like proteins.
        Proc. Natl. Acad. Sci. U.S.A. 1979; 76: 5005-5009
        • Fishman J.
        • Fishman J.H.
        • Nisselbaum J.S.
        • et al.
        Measurement of the estradiol receptor in human breast tissue by the immobilized antibody method.
        J. Clin. Endocrinol. Metab. 1975; 40: 724-727
        • Floridi A.
        • Citro G.
        • Sega E.
        • et al.
        Quantitation of estradiol receptors employing a sensitive radioimmunoassay.
        Immunol. Commun. 1975; 4: 603-615
        • Freedman B.
        • Hawkins R.A.
        Sedimentation profiles of the oestrogen receptor from breast tumors of man and rat using vertical tube rotor centrifugation.
        J. Endocrinol. 1980; 36: 431-442
        • Garola R.E.
        • McGuire W.L.
        An improved assay for nuclear estrogen receptor in experimental and human breast cancer.
        Cancer Res. 1977; 37: 3333-3337
        • Garola R.E.
        • McGuire W.L.
        A hydroxylapatite micromethod for measuring estrogen receptor in human breast cancer.
        Cancer Res. 1978; 38: 2216-2220
        • Ghosh L.
        • Ghosh B.C.
        • Das Gupta N.
        Immunocytochemical localization of estrogen in human mammary carcinoma cells by horseradish anti-horseradish peroxidase complex.
        J. Surg. Oncol. 1978; 10: 221-224
        • Ginsberg G.S.
        • Hourani M.H.
        • Gharbo S.A.
        • et al.
        The thyrotropin-binding inhibiting immunoglobulin (TBI) assay in the diagnosis and management of Graves’ disease.
        J. Clin. Immunoassay. 1983; 6: 33-41
        • Godefroi V.C.
        • Brooks S.G.
        Improved gel filtration method for analysis of estrogen receptor binding.
        Anal. Biochem. 1973; 51: 335-344
        • Greene G.L.
        • Fitch F.W.
        • lensen E.V.
        Monoclonal antibodies to estrophilin: Probes for the study of estrogen receptors.
        Proc. Natl. Acad. Sci. U.S.A. 1980; 77: 5115-5119
        • Greene G.L.
        • Nolan C.
        • Engler J.P.
        • et al.
        Monoclonal antibodies to human estrogen receptor.
        Proc. Nath Acad. Sci. U.S.A. 1980; 77: 157-161
        • Greene G.L.
        • Jensen E.V.
        Monoclonal antibodies as probes for estrogen receptor detection and characterization.
        J. Steroid Biochem. 1982; 16: 353-359
        • Hahnei R.
        • Twaddle E.
        Estradiol binding by human breast carcinoma cytosols: The influence of commonly used reference parameters on the results.
        Eur. J. Cancer. 1978; 14: 125-131
        • Hanson R.N.
        • Seitz D.E.
        • Botarro J.C.
        E-17-[125I]iodovinylestradiol: An estrogen-receptor seeking radiopharmaceutical.
        J. Nucl. Med. 1982; 23: 431-436
        • Horwitz K.B.
        • McGuire W.L.
        Specific progesterone receptors in human breast cancer.
        Steroids. 1975; 25: 497-505
      1. Hospelhorn, V. D., Shida, T., DeSombre, R. E., et al.: Nuclear estrophilin: Estimation by a novel exchange of bound estradiol. Endocrinol. Soc. Abst., 60th Annual Meeting, #521:335, 1978.

        • Jensen E.V.
        • DeSombre E.R.
        • Jungblut P.W.
        Estrogen receptors in hormone-responsive tissues and tumors.
        in: Wissler R.W. Dao T.L. Wood Jr., S. Endogenous Factors Influencing Host-Tumor Balance. University of Chicago Press, Chicago1967: 15-30
        • Jungblut P.W.
        • Hughes S.
        • Hughes A.
        • et al.
        Evaluation of various methods for the assay of cytoplasmic estrogen receptors in extracts of calf uteri and human breast cancers.
        Acta Endocrinol. 1972; 70: 185-195
        • Katzenellenbogen J.A.
        • McElvany K.D.
        • Senderoff S.G.
        • et al.
        16-[77Br]bromo-ll-methoxyestradiol-17B: A gamma-emitting estrogen imaging agent with high uptake and retention by target organs.
        J. Nucl. Med. 1982; 23: 411-419
        • Kirkland J.L.
        • Mukku V.
        • Hardy M.
        • et al.
        Evidence for triiodothyronine receptors in human endometrium and myometrium.
        Am. J. Obstet. Gynecol. 1983; 146: 380-383
      2. Kohn, L. D., Tombaccini, D., DeLuca, M. L., et al.: Monoclonal antibodies to receptors. In Greaves, M. F. (ed): London, Chapman and Hall Ltd., 1984 (in press).

        • Korenman S.G.
        • Dukes B.A.
        Specific estrogen binding by the cytoplasm of human breast carcinoma.
        J. Clin. Endocrinol. Metab. 1970; 30: 639-645
        • Kotulla P.
        • Schleusener H.
        Solubilized TSH receptor: Its usefulness for the RRA for TSH and TSH-displacing antibody.
        J. Endocrinol. Invest. 1981; 4: 155-160
        • McElvany K.D.
        • Carlson D.E.
        • Welch M.J.
        • et al.
        In vivo comparison of 16-[77Br] bromo-estradiol-17B and 16-[125I]iodoestradiol-17B.
        J. Nucl. Med. 1982; 23: 420-424
        • McGuire W.L.
        Steroid receptors and clinical breast cancer.
        in: Bresciani F. Perspectives in Steroid Receptor Research. Raven Press, New York1980: 239-246
        • Masters J.R.
        • Hawkins R.A.
        • Sangster K.
        • et al.
        Oestrogen receptors: Cellularity, elastosis and menstrual status in human breast cancer.
        Eur. J. Cancer. 1978; 14: 303-307
        • Mehdi S.Q.
        • Nussey S.S.
        A radio-ligand receptor assay for the long-acting thyroidstimulator.
        Biochem. J. 1975; 145: 105-111
      3. Mercer, W. D., Chamness, G. C., and McGuire, W. L.: Estrogen and progesterone assays in breast cancer. Laboratory Management, 17–28, 1980.

        • Mercer W.D.
        • Lippman M.
        • Wahl T.M.
        • et al.
        The use of immunocytochemical techniques for the detection of steroid hormones in breast cancer cells.
        Cancer. 1980; 46: 2859-2868
        • Oppenheimer J.H.
        • Koerner D.
        • Schwartz H.L.
        • et al.
        Specific nuclear triiodothyronine binding site in liver and kidney.
        J. Clin. Endocrinol. Metab. 1972; 35: 330-333
        • Oxcley D.K.
        • Haven G.T.
        • Wittliff J.L.
        • et al.
        Precision in estrogen and progesterone receptor assays. Results of the First CAP Pilot Survey.
        Am. J. Clin. Pathol. 1982; 78: 587-596
        • Pavlik E.J.
        • van Nagall J.R.
        • Muncey M.
        • et al.
        Rapid analysis of estrogen and progesterone receptors using gel-exclusion high performance liquid chromatography.
        Biochemistry. 1982; 21: 139-145
        • Pertschuk L.P.
        • Tobin E.H.
        • Gaetjens E.
        • et al.
        Histochemical assay of steroid hormone receptors.
        in: Bresciani F. Perspectives in Steroid Receptor Research. Raven Press, New York1980: 299-307
        • Pontes J.E.
        • Kerr J.P.
        • Kirdani R.Y.
        • et al.
        Estrogen receptors and clinical correlations with human prostatic disease.
        Urology. 1982; 19: 399-403
        • Saez S.
        • Martin P.M.
        • Chouvet C.D.
        Estradiol and progesterone receptor levels in relation to plasma estrogen and progesterone levels.
        Cancer Res. 1978; 33: 3468-3473
        • Sato B.
        • Nomura Y.
        • Nakao K.
        • et al.
        DNA binding ability of estrogen receptor from human breast cancer.
        J. Steroid Biochem. 1981; 14: 295-303
        • Seibert K.
        • Lippman M.E.
        Hormone receptor assays in breast cancer.
        J. Clin. Immunoassay. 1983; 6: 5-16
        • Smith B.R.
        • Hall R.
        Measurements of thyrotropin receptor antibodies.
        Methods Enzymol. 1981; 74: 405-420
        • Smith R.G.
        Quality control of estradiol receptor analysis.
        Cancer. 1980; 46: 2822-2824
        • Smith R.G.
        • Sestili M.A.
        Methods for ligand-receptor assays in clinical chemistry: A review.
        Clin. Chem. 1980; 26: 543-550
        • Sutherland E.W.
        Studies on the mechanism of hormone action.
        Science. 1972; 177: 401-408
        • Syne J.S.
        • Markaverich B.M.
        • Clark J.H.
        • et al.
        Estrogen binding sites in the nucleus of normal and malignant human tissue: I. Optimization of an exchange assay for the measurement of specific binding.
        Cancer Res. 1982; 42: 4443-4448
        • Syne J.S.
        • Markaverich B.M.
        • Clark J.H.
        • et al.
        Estrogen binding sites in the nucleus of normal and malignant human tissue: II. Characteristics of the multiple nuclear binding sites.
        Cancer Res. 1982; 42: 4449-4454
        • Syne J.S.
        • Panko W.B.
        New techniques for the measurement of estrogen receptors in human breast cancer.
        J. Clin. Immunoassay. 1983; 6: 17-23
        • Toft D.O.
        • Wahner H.W.
        Radiochemical probes for steroid hormone receptors.
        J. Nucl. Med. 1982; 23: 451-453
        • Traish A.M.
        • Muller R.E.
        • Wotiz H.H.
        A new procedure for the quantitation of nuclear and cytoplasmic androgen receptors.
        J. Biol. Chem. 1981; 256: 12028-12033
        • Valenstein S.L.
        • Voight W.
        • Vogel
        • et al.
        Reliability of estrogen receptors in predicting hormonal responsiveness in treatment of metastatic breast cancer.
        J. Fla. Med. Assoc. 1979; 66: 1144-1148
        • Wagner R.K.
        • Jungblut P.W.
        Quality control in steroid hormone receptor analysis.
        Cancer. 1980; 46: 2950-2952
        • Wittliff J.L.
        • Durant J.R.
        • Fisher B.
        Methods of steroid receptor analysis and their quality control in the clinical laboratory.
        in: Soto R.J. DeNicola A. Blaquier J. Physiopathology of Endocrine Diseases and Mechanism of Hormone Action. A. R. Liss, New York1981: 397-411
        • Wrange D.
        • Humla S.
        • Ramberg I.
        • et al.
        Progestin-receptor analysis in human breast cancer cytosol by isoelectric focusing in slabs of polyacrylamide gel.
        J. Steroid Biochem. 1981; 14: 141-148
        • Zakarija M.
        • McKenzie J.M.
        Assays for thyroid-stimulating antibody and their clinical application.
        in: Soto R.J. DeNicola A. Blaquier J. Physiopathology of Endocrine Diseases and Mechanism of Hormone Action. A. R. Liss, New York1981: 147-152
        • Zava D.T.
        • Harrington N.Y.
        • McGuire W.L.
        Nuclear estradiol receptor in the adult rat uterus: A new exchange assay.
        Biochemistry. 1976; 15: 42924297