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Research Article| Volume 5, ISSUE 1, P19-58, March 1985

Packaged Commercial Bacterial Identification Systems

      This paper is only available as a PDF. To read, Please Download here.
      The authors attempt to provide a useful and current guide for clinical microbiologists who are seeking a review of the available packaged commercial systems for bacterial identification. Each system is outlined according to test principle, laboratory procedures, turn-around time, and limitations. Selected peer evaluations and references for many of the systems are also cited.
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        • Reddick A.
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        • Rench M.A.
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        • Smith K.A.
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        • Tilton R.C.
        • Dias F.
        • Ryan R.W.
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        • Welch D.F.
        • Hensel D.
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        • Ahearn C.G.
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        • Buesching W.J.
        • Kurek K.
        • Roberts G.D.
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        • Edberg S.C.
        • Melton E.
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