Research Article| Volume 5, ISSUE 3, P589-613, September 1985

The Production of Monoclonal Antibodies Against Respiratory Syncytial Virus and Its Clinical Applications

  • Brian Wu
    Department of Laboratories, Albert Einstein Medical Center, Tabor and York Roads, Philadelphia, Pennsylvania 19141
    Director, Virology and Flow Cytometer Laboratories, Department of Laboratories, Albert Einstein Medical Center, Northern Division, Philadelphia, Pennsylvania
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      The availability of monoclonal antibodies against various viral components, either internal nucleoproteins or membrane proteins, has greatly advanced our understanding of the biochemistry of respiratory syncytial virus (RSV). The components responsible for serum neutralization have been identified by monoclonal antibodies, thus facilitating the development of synthetic RSV vaccine. A number of clinical tests have been devised using monoclonal antibodies for either qualitative or quantitative determination of the presence of RSV antigens. The practical procedures for the widely used indirect immunofluorescent antibody (IFA) and enzyme-linked immunosorbent assays (ELISA) are presented. Various commercial ELISA spectrophotometers and washers are also compared and evaluated.
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