Advertisement
Research Article| Volume 5, ISSUE 3, P561-574, September 1985

Use of Monoclonal Antibodies in the Diagnosis and Epidemiologic Studies of Legionellosis

DOI:https://doi.org/10.1016/S0272-2712(18)30860-6
Use of Monoclonal Antibodies in the Diagnosis and Epidemiologic Studies of Legionellosis
Previous ArticleThe Detection of Streptococcal Antigens Using Monoclonal Antibodies
Next ArticleMonoclonal Antibodies for the Diagnosis of Sexually Transmitted Diseases
      This paper is only available as a PDF. To read, Please Download here.
      The production of monoclonal antibodies against Legionella pneumophila has been reported by numerous authors in the last few years. Despite this fact, there are still no well-defined areas iii which they can be used in the clinical microbiology laboratory. Although some of these antibodies can be expected to reach the market within the next few years, their exact value will have to be critically assessed before they are used routinely.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribers receive full online access to your subscription and archive of back issues up to and including 2002.

      Content published before 2002 is available via pay-per-view purchase only.

      Subscribe:

      Subscribe to Clinics in Laboratory Medicine
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Register: Create an account
      Institutional Access: Sign in to ScienceDirect

      References

        • Anand C.M.
        • Skinner A.R.
        • Malic A.
        Interaction of Legionella pneumophila and a free living amoeba (Acanthamoeha palestinensis).
        J. Hyg. Camb. 1983; 91: 167-178
        • Berdal B.P.
        • Farshy C.E.
        • Feeley J.C.
        Detection of Legionella pneumophila antigen in urine by enzyme-linked immunospecific assay.
        J. Clin. Microbiol. 1979; 9: 575-578
        • Bibb W.F.
        • Arnow P.M.
        • Dellinger D.L.
        • et al.
        Isolation and characterization of a seventh serogroup of Legionella pneumophila.
        J. Clin. Microbiol. 1983; 17: 346-348
        • Bibb W.F.
        • Arnow P.M.
        • Thacker L.
        • et al.
        Detection of soluble Legionella pneumophila antigens in serum and urine specimens by enzyme-linked immunosorbent assay with monoclonal and polyclonal antibodies.
        J. Clin. Microbiol. 1984; 20: 478-482
        • Bibb W.F.
        • Sorg R.J.
        • Thomason B.M.
        • et al.
        Recognition of a second serogroup of Legionella longbeachae.
        J. Clin. Microbiol. 1983; 14: 674-677
        • Bisset M.L.
        • Lee J.O.
        • Lindquist D.S.
        A new serogroup of Legionella pneumophila: Serogroup 8.
        J. Clin. Microbiol. 1983; 17: 887-891
        • Brenner D.J.
        Classification of legionellae. Proc. 2nd International Symposium on Legionella.
        American Society for Microbiology, Washington, D.C.1984
        • Brenner D.J.
        • Steigerwalt A.G.
        • Gorman G.W.
        • et al.
        Legionella bozemanii sp. nov. and legionella dumoffii sp. nov.: Classification of two additional species of Legionella associated with human pneumonia.
        Curr. Top. Microbiol. Immunol. 1980; 4: 111-116
        • Brenner D.J.
        • Steigerwalt A.G.
        • McDade J.E.
        Classification of the Legionnaires’ disease bacterium: Legionella pneumophila, genus novum, species nova of the family Legionellaceae, familia nova.
        Ann. Intern. Med. 1979; 90: 656-658
        • Brown S.L.
        • Bibb W.F.
        • McKinney R.M.
        Retrospective examination of lung tissue specimen for the presence of Legionella organisms: Comparison of an indirect fluorescent-antibody system with direct fluorescent-antibody testing.
        J. Clin. Microbiol. 1984; 19: 468-472
        • Brown A.
        • Garrity G.M.
        • Vickers R.M.
        Fluoribacter dumoffii (Brenner et al.) comb. nov. and Fluoribacter gormanii (Morris et al.) comb. nov.
        Int. J. Syst. Bacteriol. 1981; 31: 111-115
        • Brown A.
        • Vickers R.M.
        • Elder E.M.
        • et al.
        Plasmid and surface antigen markers of endemic and epidemic Legionella pneumophila strains.
        J. Clin. Microbiol. 1982; 16: 230-235
        • Brown A.
        • Yu V.L.
        • Magnussen M.H.
        • et al.
        Isolation of Pittsburgh pneumonia agent from a hospital shower.
        Appl. Environ. Microbiol. 1982; 43: 725-726
        • Campbell J.
        • Bibb W.F.
        • Lambert M.A.
        • et al.
        Legionella sainthelensi: A new species of Legionella isolated from water near Mt.
        St. Helens. Appl. Environ. Microbiol. 1984; 47: 369-373
        • Cherry W.B.
        • Gorman G.W.
        • Orrison L.H.
        • et al.
        Legionella jordanis: A new species of Legionella isolated from water and sewage.
        J. Clin. Microbiol. 1982; 15: 290-297
        • Cherry W.B.
        • Pittman B.
        • Harris P.P.
        • et al.
        Detection of Legionnaires’ disease bacteria by direct immunofluorescent staining.
        J. Clin. Microbiol. 1978; 3: 329-338
        • Collins M.T.
        • Espersen F.
        • Hpiby N.
        • et al.
        Crossed immunoelectrophoretic analysis of Legionella pneumophila serogroup 1 antigens.
        Infect. Immun. 1983; 39: 1428-1440
        • Cordes L.G.
        • Fraser D.W.
        • Skaliy P.
        • et al.
        Legionnaires’ disease outbreak at an Atlanta, Georgia country club: Evidence for spread from an evaporative condenser.
        Am. J. Epidemiol. 1980; 111: 425-431
        • de Lalla F.
        • Rossini G.
        • Giannattasio G.
        • et al.
        Legionnaires’ disease in an Italian hotel.
        Lancet. 1980; 2: 1187
        • Desplaces N.
        • Nahapetian K.
        • Dournon E.
        Inventaire des Legionella dans l’environnement parisien: Implications pratiques.
        Presses Méd. 1984; 13: 1875-1879
        • Dondero T.J.
        • Rendtorff R.C.
        • Mallison G.F.
        • et al.
        Outbreak of Legionnaires’ disease associated with a contaminated air conditioning cooling tower.
        N. Engl. J. Med. 1980; 302: 365-370
        • Dutka B.J.
        • Walsh K.
        Incidence of Legionella organisms in selected Ontario (Canada) cities.
        Sei. Total Environ. 1984; 39: 237-249
        • Edelstein P.H.
        Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens.
        J. Clin. Microbiol. 1981; 14: 298-303
        • Edelstein P.H.
        • Bibb W.F.
        • Gorman G.W.
        • et al.
        Legionella pneumophila serogroup 9: A cause of human pneumonia.
        Ann. Intern. Med. 1984; 101: 196-199
        • Edelstein P.H.
        • Brenner D.J.
        • Moss C.W.
        • et al.
        Legionella wadsworthii species nova: A cause of human pneumonia.
        Ann. Intern. Med. 1982; 97: 809-813
        • Editorial
        Waterborne Legionella.
        Lancet. 1983; 2: 381-383
        • England III, A.C.
        • McKinney R.M.
        • Skaliy P.
        • et al.
        A fifth serogroup of Legionella pneumophila.
        Ann. Intern. Med. 1980; 93: 58-59
        • Feeley J.C.
        • Gibson R.J.
        • Gorman G.W.
        Charcoal-yeast extract agar: A primary isolation medium for Legionella pneumophila.
        J. Clin. Microbiol. 1979; 10: 436-441
        • Fisher-Hoch S.P.
        • Smith M.G.
        • Colbourn J.S.
        Legionella pneumophila in hospital hot-water cylinders.
        Lancet. 1982; 1: 1073
        • Fliermans C.B.
        • Cherry W.B.
        • Orrison L.H.
        • et al.
        Ecological distribution of Legionella pneumophila.
        Appl. Environ. Microbiol. 1981; 42: 9-16
        • Fliermans C.B.
        • Cherry W.B.
        • Orrison L.H.
        • et al.
        Isolation of Legionella pneumophila from nonepidemic-related aquatic habitats.
        Appl. Environ. Microbiol. 1979; 37: 1239-1242
        • Fraser D.W.
        Sources of legionellosis. Proc. 2nd International Symposium on Legionella.
        American Society for Microbiology, Washington, D.C.1984
        • Fraser D.W.
        • Tsai T.R.
        • Orenstein W.
        • et al.
        Legionnaires’ disease: Description of an epidemic of pneumonia.
        N. Engl. J. Med. 1977; 297: 1189-1197
        • Garrity G.M.
        • Brown A.
        • Vickers R.M.
        Tatlockia and Fluoribacter: Two new genera of organisms resembling Legionella pneumophila.
        Int. J. Syst. Bacteriol. 1980; 30: 609-614
        • Glick T.H.
        • Gregg M.B.
        • Berman B.
        • et al.
        Pontiac fever: An epidemic of unknown etiology in a health department: I. Clinical and epidemiologic aspects.
        Am. J. Epidemiol. 1978; 107: 149-160
        • Gosting L.H.
        • Cabrian K.
        • Sturge J.C.
        • et al.
        Identification of a species-specific antigen in Legionella pneumophila by a monoclonal antibody.
        J. Clin. Microbiol. 1984; 20: 1031-1035
        • Guillet J.-G.
        • Hoebeke J.
        • Tram C.
        • et al.
        Characterization, serological specificity, and diagnostic possibilities of monoclonal antibodies against Legionella pneumophila.
        J. Clin. Microbiol. 1983; 18: 793-797
        • Hébert G.A.
        • Steigerwalt A.G.
        • Brenner D.J.
        Legionella micdadei, species nova: Classification of a third species of Legionella associated with human pneumonia.
        Curr. Microbiol. 1980; 3: 255-257
        • Hébert G.A.
        • Thomason B.M.
        • Harris P.P.
        • et al.
        “Pittsburgh pneumonia agent”: A bacterium phenotypically similar to Legionella pneumophila and identical to the TATLOCK bacterium.
        Ann. Intern. Med. 1980; 92: 53-54
        • Herwalt L.A.
        • Gorman G.W.
        • McGrath T.
        • et al.
        A new Legionella species, Legionella feeleii species nova, causes Pontiac fever in an automobile plant.
        Ann. Intern. Med. 1984; 100: 333-338
        • Hsu S.C.
        • Martin R.
        • Wentworth B.B.
        Isolation of Legionella species from drinking water.
        Appl. Environ. Microbiol. 1984; 43: 830-832
        • Joly J.R.
        • Boissinot M.
        • Duchaine J.
        • et al.
        Ecological distribution of Legionellaceae in the Quebec City area.
        Can. J. Microbiol. 1983; 30: 63-67
        • Joly J.R.
        • Chen Y.Y.
        • Ramsay D.
        Serogrouping and subtyping of Legionella pneumophila with monoclonal antibodies.
        J. Clin. Microbiol. 1983; 18: 1040-1046
        • Joly J.R.
        • Dewailly E.
        • Bernard L.
        • et al.
        Legionella and domestic hot-water heaters.
        Can. Med. Assoc. J. 1985; 132: 160

      45. Joly, J. R., McKinney, R. M., and Watkins, I. D.: Monoclonal Antibodies to Legionella pneumophila: Possible Applications in Clinical Diagnostic Tests and Epidemiologic Investigations. New York, Academic Press, (in press).

        • Joly J.R.
        • Winn W.C.
        Correlation of subtypes of Legionella pneumophila defined by monoclonal antibodies with epidemiological classification of cases and environmental sources.
        J. Infect. Dis. 1984; 150: 667-671
        • Kohler G.
        • Milstein C.
        Continuous cultures of fused cells secreting antibody of predefined specificity.
        Nature (London). 1975; 256: 495-497
        • Lema M.
        • Brown A.
        Electrophoretic characterization of soluble protein extracts of Legionella pneumophila and others of the family Legionellaceae.
        J. Clin. Microbiol. 1983; 17: 1132-1140
        • McDade J.E.
        • Shepard C.C.
        • Fraser D.W.
        • et al.
        Legionnaires’ disease: Isolation of a bacterium and demonstration of its role in other respiratory disease.
        N. Engl. J. Med. 1977; 297: 1197-1203
        • McKinney R.M.
        • Porschen R.K.
        • Edelstein P.H.
        • et al.
        Legionella longbeachae sp. nov., another etiologic agent of human pneumonia.
        Ann. Intern. Med. 1981; 94: 739-743
        • McKinney R.M.
        • Thacker L.
        • Harris P.P.
        • et al.
        Four serogroups of Legionnaires’ disease bacteria defined by immunofluorescence.
        Ann. Intern. Med. 1979; 90: 621-624
        • McKinney R.M.
        • Thacker L.
        • Wells D.E.
        • et al.
        Monoclonal antibodies to Legionella pneumophila serogroup 1: Possible applications in diagnostic tests and epidemiologic studies.
        Zentralbl. Bakterial. Mikrobiol. Hyg. 1983; 255: 91-95
        • McKinney R.M.
        • Wilkinson H.W.
        • Sommers H.W.
        • et al.
        Legionella pneumophila serogroup 6: Isolation from cases of legionellosis, identification by immunofluorescence staining, and immunological response to infection.
        J. Clin. Microbiol. 1980; 12: 395-401
        • Morris G.K.
        • Patton C.M.
        • Feeley J.C.
        Isolation of the Legionnaires’ disease bacterium from environmental samples.
        Ann. Intern. Med. 1979; 90: 664-666
        • Morris G.K.
        • Steigerwalt A.G.
        • Feeley J.C.
        • et al.
        Legionella gormanii sp. nov.
        J. Clin. Microbiol. 1980; 12: 718-721
        • Orrison L.H.
        • Bibb W.B.
        • Cherry W.B.
        • et al.
        Determination of antigenic relationships among Legionellae and non-Legionellae by direct fluorescent-antibody and immunodiffusion tests.
        J. Clin. Microbiol. 1983; 17: 332-337
        • Orrison L.H.
        • Cherry W.B.
        • Tyndall R.L.
        • et al.
        Legionella oakridgensis: Unusual species isolated from cooling tower water.
        Appl. Environ. Microbiol. 1983; 45: 536-545
        • Para M.F.
        • Plouffe J.F.
        Production of monoclonal antibodies to Legionella pneumophila serogroups 1 and 6.
        J. Clin. Microbiol. 1983; 18: 895-900
        • Pasculle A.W.
        • Feeley J.C.
        • Gibson R.G.
        • et al.
        Pittsburgh pneumonia agent: Direct isolation from human lung tissue.
        J. Infect. Dis. 1980; 141: 727-732
        • Plouffe J.F.
        • Para M.F.
        • Maher W.E.
        • et al.
        Subtypes of Legionella pneumophila serogroup 1 associated with different attack rates.
        Lancet. 1983; 2: 649-650
        • Reingold A.L.
        • Thomason B.M.
        • Brake B.J.
        • et al.
        Legionella pneumophila in the United States: The distribution of serogroups and species causing human illness.
        J. Infect. Dis. 1984; 149: 819
        • Rowbotham T.J.
        Preliminary report on the pathogenicity of Legionella pneumophila for freshwater and soil amoeba.
        J. Clin. Pathol. 1980; 33: 1179-1183
        • Sethi K.K.
        • Drueke V.
        • Brandis H.
        Hybridoma-derived monoclonal immunoglobulin M antibodies to Legionella pneumophila serogroup 1 with diagnostic potential.
        J. Clin. Microbiol. 1983; 17: 953-957
        • Stout J.
        • Yu V.L.
        • Vickers R.M.
        • et al.
        Ubiquitousness of Legionella pneumophila in the water supply of a hospital with endemic Legionnaires’ disease.
        N. Engl. J. Med. 1882; 306: 466-468
        • Tang P.W.
        • Toma S.
        • Wayne Moss C.
        • et al.
        Legionella bozemanii serogroup 2: A new etiological agent.
        J. Clin. Microbiol. 1984; 19: 30-33
        • Thomason B.M.
        • Bibb W.F.
        Use of absorbed antisera for demonstration of antigenic variation among strains of Legionella pneumophila serogroup 1.
        J. Clin. Microbiol. 1984; 19: 794-797
        • Tilton R.C.
        Legionnaires’ disease antigen detected by enzyme-linked immunosorbent assay.
        Ann. Intern. Med. 1979; 90: 697-698
        • Tison D.L.
        • Pope D.H.
        • Cherry W.B.
        • et al.
        Growth of Legionella pneumophila in association with blue-green algae.
        Appl. Environ. Microbiol. 1980; 39: 456-459
        • Tobin J.O.’H.
        • Beare J.
        • Dunnill M.S.
        • et al.
        Legionnaires’ disease in a transplant unit: Isolation of the causative agent from shower baths.
        Lancet. 1980; 2: 118-121
        • van Ketel R.J.
        • ter Schegget J.
        • Zanen H.C.
        Molecular epidemiology of Legionella pneumophila serogroup 1.
        J. Clin. Microbiol. 1984; 20: 362-364
        • Wadowsky R.
        • Yee R.B.
        Glycine containing selective medium for isolation of Legionellaceae from environmental specimens.
        Appl. Environ. Microbiol. 1981; 42: 768-772
        • Wadowsky R.M.
        • Yee R.B.
        • Mezmar L.
        • et al.
        Hot-water systems as source of Legionella pneumophila in hospital and nonhospital plumbing fixtures.
        Appl. Environ. Microbiol. 1982; 43: 1104-1110
        • Watkins I.D.
        • Tobin J.O.’H.
        Studies with monoclonal antibodies to Legionella species. Proc. 2nd International Symposium on Legionella.
        American Society for Microbiology, Washington, D.C.1984

      74. Watkins, I. D., and Tobin, J. O’ H.: Studies with Legionella pneumophila serogroup 1 monoclonal antibodies: Communicable Disease Report. Canadian Public Health Laboratory Service. CDR 83-49 9th December 1983.

        • Wilkinson H.W.
        • Reingold A.L.
        • Brake B.J.
        • et al.
        Reactivity of serum from patients with suspected legionellosis against 29 antigens of Legionellaceae and Legionella-like organisms by indirect immunofluorescence assay.
        J. Infect. Dis. 1983; 147: 23-31
        • Zanem-Lim O.G.
        • van den Broek N.J.
        • Rietra P.J.G.M.
        • et al.
        Comparison of strains of Legionella pneumophila serogroup 1 isolated in four hospitals from patients and hot-water supplies.
        J. Infect. Dis. 1984; 150: 508-512

      Article info

      Identification

      DOI: https://doi.org/10.1016/S0272-2712(18)30860-6

      Copyright

      © 1985 Elsevier Inc. Published by Elsevier Inc. All rights reserved.

      ScienceDirect

      Access this article on ScienceDirect

      The content on this site is intended for healthcare professionals.


      We use cookies to help provide and enhance our service and tailor content. To update your cookie settings, please visit the Cookie Preference Center for this site.
      Copyright © 2023 Elsevier Inc. except certain content provided by third parties.


      RELX