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Research Article| Volume 5, ISSUE 3, P513-529, September 1985

Nucleic Acid Hybridization in the Diagnosis of Viral Infections

  • Marie L. Landry
    Correspondence
    Corresponding author: Virology Reference Laboratory, Veterans Administration Medical Center, West Spring Street, West Haven, Connecticut 06516
    Affiliations
    Associate Director, Virology Reference Laboratory, Veterans Administration Medical Center, West Haven, Connecticut

    Assistant Professor, Laboratory Medicine and Medicine, Yale University School of Medicine, New Haven, Connecticut
    Search for articles by this author
  • Caroline K.Y. Fong
    Affiliations
    Section Chief, Immunodiagnosis and Electron Microscopy, Virology Reference Laboratory, West Haven, Connecticut

    Research Scientist, Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut
    Search for articles by this author
DOI:https://doi.org/10.1016/S0272-2712(18)30857-6
Nucleic Acid Hybridization in the Diagnosis of Viral Infections
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      Nucleic acid hybridization technology is now being applied to clinical virology, both for rapid diagnosis using clinical specimens and for studies of viral pathogenesis. This review deals with the methods used, their applications, the results obtained, and the problems encountered.
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      References

        • Andiman W.
        • Gradoville L.
        • Heston L.
        • et al.
        Use of cloned probes to detect Epstein-Barr viral DNA in tissues of patients with neoplastic and lymphoproliferative diseases.
        J. Infect. Dis. 1983; 148: 967-977
        • Bornkamm G.W.
        • Desgranges C.
        • Gissman L.
        Nucleic acid hybridization for the detection of viral genomes.
        Curr. Top. Microbiol. Immunol. 1983; 104: 287-298
        • Brandsman J.
        • Miller G.
        Nucleic acid and spot hybridization: Rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA.
        Proc. Natl. Acad. Sei. U.S.A. 1980; 77: 6851-6855
        • Brigati D.J.
        • Myerson D.
        • Leary J.J.
        • et al.
        Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes.
        Virology. 1983; 126: 32-50
        • Chou S.
        • Merigan T.C.
        Rapid detection and quantitation of human cytomegalovirus in urine through DNA hybridization.
        N. Engl. J. Med. 1982; 308: 921-925
        • Durst M.
        • Gissmann L.
        • Ikenberg H.
        • et al.
        A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions.
        Proc. Natl. Acad. Sci. U.S.A. 1983; 80: 3812-3815
        • Enders J.F.
        • Weller T.H.
        • Robbins F.C.
        Cultivation of the Lansing strain of poliomyelitis virus in cultures of various human embryonic tissues.
        Science. 1949; 109: 85-87
        • Flores J.
        • Purcell R.H.
        • Perez J.
        • et al.
        A dot hybridization assay for detection of rotavirus.
        Lancet. 1983; 1: 555-558
        • Gadler H.
        Nucleic acid hybridization for measurement of effects of antiviral compounds on human cytomegalovirus DNA replication.
        Antimicrob. Agents Chemother. 1983; 24: 370-374
        • Gadler H.
        • Larsson A.
        • Solver E.
        Nucleic acid hybridization, a method to determine effects of antiviral compounds on herpes simplex virus type 1 DNA synthesis.
        Antiviral Res. 1984; 4: 63-70
        • Gall J.G.
        • Pardue M.L.
        Nucleic acid hybridization in cytological preparations.
        Methods Enzymol. 1971; 21: 470-480
        • Gelb L.D.
        • Kohne D.E.
        • Martin M.A.
        Quantitation of simian virus 40 sequences in African Green Monkey, mouse, and virus-transformed cell genomes.
        J. Mol. Biol. 1971; 57: 129-145
        • Gillespie D.
        • Spiegelman S.
        A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
        J. Mol. Biol. 1965; 12: 829-842
        • Gissmann L.
        • Wolnik L.
        • Ikenberg H.
        • et al.
        Human papillomavirus types 6 and 11 DNA sequences in genital and laryngeal papillomas and in some cervical cancers.
        Proc. Natl. Acad. Soci U.S.A. 1983; 80: 560-563
        • Goldin A.L.
        • Sandri-Goldin R.M.
        • Levine M.
        • et al.
        Cloning of herpes simplex virus type 1 sequences representing the whole genome.
        J. Virol. 1981; 38: 50-58
        • Green M.
        • Maniatis T.
        • Melton D.A.
        Human B-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei.
        Cell. 1983; 32: 681-694
        • Grinnell B.W.
        • Padgett B.L.
        Distribution of nonintegrated DNA from IC papovavirus in organs of patients with progressive multifocal leukoencephalopathy.
        J. Infect. Dis. 1983; 147: 669-675
        • Grunstein M.
        • Hogness D.S.
        Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene.
        Proc. Natl. Acad. Sei. U.S.A. 1975; 72: 3961-3965
        • Hochberg F.H.
        • Miller G.
        • Schooley R.T.
        • et al.
        Central nervous system lymphoma related to Epstein-Barr virus.
        N. Engl. J. Med. 1983; 309: 745-748
        • Howley P.M.
        • Israel M.A.
        • Law M.F.
        • et al.
        A rapid method for detecting and mapping homology between heterologous DNAs.
        J. Biol. Chem. 1979; 254: 4876-4883
        • Hsiung G.D.
        • Fong C.K.Y.
        • August M.
        The use of electron microscopy in diagnosis of virus infection: An overview.
        Prog. Med. Virol. 1979; 25: 133-159
        • Hsiung G.D.
        Diagnostic Virology.
        Edition 3. Yale University Press, New Haven1982
        • Hyypia T.
        • Stålhandske P.
        • Vainionpaa R.
        • et al.
        Detection of enteroviruses by spot hybridization.
        J. Clin. Microbiol. 1984; 19: 436-438
        • Jeang K.T.
        • Hayward G.S.
        A cytomegalovirus DNA sequence containing tracts of tandemly repeated CA dinucleotides hybridizes to highly repetitive dispersed elements in mammalian cell genomes.
        Mol. Cell. Biol. 1983; 3: 1398-1402
        • Jones T.R.
        • Hyman R.W.
        Specious hybridization between herpes simplex virus DNA and human cellular DNA.
        Virology. 1983; 131: 555-560
        • Lancaster W.D.
        • Kurman R.J.
        • Sanz L.E.
        • et al.
        Human papillomavirus: Detection of viral DNA sequences and evidence for molecular heterogeneity in metaplasias and dysplasias of the uterine cervix.
        Intervirology. 1983; 20: 203-212
        • Landry M.L.
        • Mayo D.R.
        • Hsiung G.D.
        The need for a rapid and accurate viral diagnosis.
        Pharmacol. Ther. 1983; 18: 107-132
        • Langer P.R.
        • Waldrop A.A.
        • Ward D.C.
        Enzymatic synthesis of biotin-labeled polynucleotides: Novel nucleic acid affinity probes.
        Proc. Natl. Acad. Sci. U.S.A. 1981; 78: 6633-6637
        • Leary J.J.
        • Brigati D.J.
        • Ward D.C.
        Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-Blots.
        Proc. Natl. Acad. Sci. U.S.A. 1983; 80: 4045-4049
        • Lie-Injo L.E.
        • Balasegaram M.
        • Lopez C.G.
        • et al.
        Hepatitis B virus DNA in liver and white blood cells of patients with hepatoma.
        DNA. 1983; 2: 301-308
        • Maniatis T.
        • Fritsch E.F.
        • Sambrook J.
        Molecular Cloning: A Laboratory Manual.
        Cold Spring Harbor Laboratory, Cold Spring Harbor1982
        • Marmur J.
        • Doty P.
        Determination of the base composition of DNA from its thermal denaturation temperature.
        J. Mol. Biol. 1962; 5: 109-118
        • Martin D.C.
        • Katzenstein D.A.
        • Yu G.S.M.
        Cytomegalovirus viremia detected by molecular hybridization and electron microscopy.
        Ann. Intern. Med. 1984; 100: 222-225
        • McConaughy J.L.
        • Laird C.D.
        • McCarthy B.J.
        Nucleic acid reassociation in formamide.
        Biochemistry. 1969; 8: 3289-3295
        • Meinkoth J.
        • Wahl G.
        Hybridization of nucleic acids immobilized on solid supports.
        Anal. Biochem. 1984; 138: 267-284
        • Messing J.
        New M13 vectors for cloning.
        Methods Enzymol. 1983; 101: 20-78
        • Minson A.C.
        • Darby G.
        Hybridization techniques.
        in: Howard C. New Developments in Practical Virology. Alan R. Liss, New York1982
        • Myerson D.
        • Hackman R.C.
        • Nelson J.A.
        • et al.
        Widespread presence of histologically occult cytomegalovirus.
        Hum. Pathol. 1984; 15: 430-439
        • Myerson D.
        • Hackman R.C.
        • Meyers J.D.
        Diagnosis of cytomegaloviral pneumonia by in-situ hybridization.
        J. Infect. Dis. 1984; 150: 272-277
        • Peden K.
        • Mounts P.
        • Hayward G.S.
        Homology between mammalian cell DNA sequences and human herpes virus genomes detected by a hybridization procedure with high-complexity probe.
        Cell. 1982; 31: 71-80
        • Puga A.
        • Cantin E.M.
        • Notkins A.L.
        Homology between murine and human cellular DNA sequences and the terminal repetition of the S component of herpes simplex virus type 1 DNA.
        Cell. 1982; 31: 81-87
        • Ranki M.
        • Palva A.
        • Virtanen M.
        • et al.
        Sandwich hybridization as a convenient method for the detection of nucleic acids in crude samples.
        Gene. 1983; 21: 77-85
        • Redfield D.C.
        • Richman D.D.
        • Albanil S.
        • et al.
        Detection of herpes simplex virus in clinical specimens by DNA hybridization.
        Diagn. Microbiol. Infect. Dis. 1983; 1: 117-128
        • Richman D.D.
        • Cleveland P.H.
        • Redfield D.C.
        • et al.
        Rapid viral diagnosis.
        J. Infect. Dis. 1984; 149: 298-310
        • Richman D.
        • Schmidt N.
        • Plotkin S.
        • et al.
        Summary of a workshop on new and useful methods in rapid viral diagnosis.
        J. Infect. Dis. 1984; 150: 941-951
        • Rigby P.W.
        • Dieckmann M.
        • Rhodes C.
        • et al.
        Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase.
        J. Mol. Biol. 1977; 113: 237-251
        • Rotbart H.A.
        • Levin M.J.
        • Villarreal L.P.
        Use of subgenomic poliovirus DNA probes to detect the major subgroups of enteroviruses.
        J. Clin. Microbiol. 1984; 20: 1105-1108
        • Ruger R.
        • Bornkamm G.W.
        • Fleckenstein B.
        Human cytomegalovirus DNA sequences with homologies to the cellular genome.
        J. Gen. Virol. 1984; 65: 1351-1364
        • Schildkraut C.L.
        • Lifson S.
        Dependence of the melting temperature of DNA on salt concentration.
        Biopolymers. 1965; 3: 195-208
        • Scotto J.
        • Hadchouel M.
        • Hery C.
        • et al.
        Hepatitis B virus DNA in children’s liver diseases: Detection by blot hybridisation in liver and serum.
        Gut. 1983; 24: 618-624
        • Scotto J.
        • Hadchouel M.
        • Hery C.
        • et al.
        Detection of hepatitis B virus DNA in serum by a simple spot hybridization technique: Comparison with results for other viral markers.
        Hepatology. 1983; 3: 279-284
        • Seidlin M.
        • Takiff H.E.
        • Smith H.A.
        • et al.
        Detection of varicella-zoster virus by dot-blot hybridization using a molecularly cloned viral DNA probe.
        J. Med. Virol. 1984; 13: 53-61
        • Shaw G.M.
        • Hahn B.H.
        • Arya S.K.
        • et al.
        Molecular characterization of human T’-cell leukemia (lymphotropic) virus type III in the acquired immune deficiency syndrome.
        Science. 1984; 226: 1165-1171
        • Sixbey J.W.
        • Nedrud J.G.
        • Raab-Traub N.
        • et al.
        Epstein-Barr virus replication in oropharyngeal epithelial cells.
        N. Engl. J. Med. 1984; 310: 1225-1230
        • Southern E.M.
        Detection of specific sequences among DNA fragments separated by gel electrophoresis.
        J. Mol. Biol. 1975; 93: 503-517
        • Spector S.
        • Rau J.A.
        • Spector D.H.
        • et al.
        Detection of human cytomegalovirus in clinical specimens by DNA-DNA hybridization.
        J. Infect. Dis. 1984; 150: 121-126
        • Stålhandske P.
        • Pettersson U.
        Identification of DNA viruses by membrane filter hybridization.
        J. Clin. Microbiol. 1982; 15: 744-747
        • Stålhandske P.
        • Hyypia T.
        • Gadler H.
        • et al.
        The use of molecular hybridization for demonstration of adenoviruses in human stools.
        Curr. Top. Microbiol. Immunol. 1983; 104: 287-298
        • Tchen P.
        • Fuchs R.P.P.
        • Sage E.
        • et al.
        Chemically modified nucleic acids as immunodetectable probes in hybridization experiments.
        Proc. Natl. Acad. Sei. U.S.A. 1984; 81: 3466-3470
        • Thomas P.S.
        Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
        Proc. Natl. Acad. Sei. U.S.A. 1980; 77: 5201-5205
        • Virtanen M.
        • Laaksonen M.
        • Soderlund H.
        • et al.
        Novel test for rapid viral diagnosis: Detection of adenovirus in nasopharyngeal mucus aspirates by means of nucleic acid sandwich hybridization.
        Lancet. 1983; 1: 381-383
        • Virtanen M.
        • Syvanen A.C.
        • Oram J.
        • et al.
        Cytomegalovirus in urine: Detection of viral DNA by sandwich hybridization.
        J. Clin. Microbiol. 1984; 20: 1083-1088
        • Wahl G.M.
        • Stern M.
        • Stark G.R.
        Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl paper and rapid hybridization by using dextran sulfate.
        Proc. Natl. Acad. Sei. U.S.A. 1979; 76: 3683-3687
        • Weller I.V.D.
        • Fowler M.J.F.
        • Monjardino J.
        • et al.
        The detection of HBV-DNA in serum by molecular hybridization: A more sensitive method for the detection of complete HBV particles.
        J. Med. Virol. 1982; 9: 273-280
        • Wetmur J.
        • Davidson N.
        Kinetics of renaturation of DNA.
        J. Mol. Biol. 1968; 31: 349-370
        • Wong-Staal F.
        • Hahn B.
        • Manzari V.
        • et al.
        A survey of human leukaemias for sequences of a human retrovirus.
        Nature. 1983; 302: 626-628

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      DOI: https://doi.org/10.1016/S0272-2712(18)30857-6

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      © 1985 Elsevier Inc. Published by Elsevier Inc. All rights reserved.

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