Research Article| Volume 5, ISSUE 3, P503-512, September 1985

Biotin-Labeled DNA Probes for Detection of Epstein-Barr Virus by In-Situ Cytohybridization

  • John W. Sixbey
    Corresponding author: Division of Infectious Diseases, St. Jude Children’s Research Hospital, 332 N. Lauderdale St., Memphis, Tennessee 38101
    Assistant Member, Division of Infectious Diseases, St. Jude Children’s Research Hospital, Memphis, Tennessee
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  • Joseph S. Pagano
    Director, Lineberger Cancer Research Center, and Professor, Departments of Medicine and Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina
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      This paper is only available as a PDF. To read, Please Download here.
      The detection of viral nucleic acid in tissue or cytologic preparations by in-situ cytohybridization is a biochemical technique that has been moved by recent developments into the realm of diagnostic pathology. This article describes changes in the methodology of cytohybridization within the framework of the Epstein-Barr virus system and illustrates how broader application of molecular hybridization may increase the appreciation of mechanisms of disease at epidemiologic, cellular, and molecular levels.
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