Advertisement
Research Article| Volume 5, ISSUE 3, P463-473, September 1985

Use of Non radioactive DNA Probes for the Detection of infectious Bacteria

DOI:https://doi.org/10.1016/S0272-2712(18)30853-9
Use of Non radioactive DNA Probes for the Detection of infectious Bacteria
Previous ArticleDNA Hybridization in the Diagnosis of Bacterial Diarrhea
Next ArticleMicrobial Diagnosis by Nucleic Acid Sandwich Hybridization
      This paper is only available as a PDF. To read, Please Download here.
      Application of the biotinylated DNA probe systems in the detection of Chlamydia trachomatis and penicillin-resistant Neisseria gonorrhoeae is presented. The three formats employed are the dot-blot, in-situ, and Southern hybridization.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribers receive full online access to your subscription and archive of back issues up to and including 2002.

      Content published before 2002 is available via pay-per-view purchase only.

      Subscribe:

      Subscribe to Clinics in Laboratory Medicine
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Register: Create an account
      Institutional Access: Sign in to ScienceDirect

      References

        • Bancroft J.D.
        • Stevens A.
        Theory and Practice of Histological Techniques, Edition 2.
        Churchill Livingstone, Edinburgh1982
        • Berninger M.
        • Hammer M.
        • Hoyer B.
        • et al.
        An assay for the detection of the DNA genome of hepatitis B in serum.
        J. Med. Virol. 1982; 9: 57-68
        • Blum H.E.
        • Stowring L.
        • Figus A.
        • et al.
        Detection of hepatitis B virus DNA in hepatocytes, bile duct epithelium, and vascular elements by in situ hybridization.
        Proc. Natl. Acad. Sci. U.S.A. 1983; 80: 6685-6688
        • Brandsma J.
        • Miller G.
        Nucleic acid spot hybridization: Rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA.
        Proc. Natl. Acad. Sci. U.S.A. 1980; 77: 6851-6855
        • Brechot C.
        • Pourcel C.
        • Louise A.
        • et al.
        Presence of integrated hepatitis B virus DNA sequence in cellular DNA of human hepatocellular carcinoma.
        Nature. 1980; 286: 533-535
        • Brechot C.
        • Hadchouel M.
        • Scotto J.
        • et al.
        State of hepatitis B virus DNA in hepatocytes of patients with hepatitis B surface antigen-positive and-negative liver diseases.
        Proc. Natl. Acad. Sci. U.S.A. 1981; 78: 3906-3910
        • Brenner D.J.
        • Martin M.A.
        • Hoyer B.H.
        Deoxyribonucleic acid homologies among some bacteria.
        J. Bacteriol. 1967; 94: 486-487
        • Brenner D.J.
        • Fanning G.R.
        • Johnson K.E.
        • et al.
        Polynucleotide sequence relationship among members of Enterobacteriaceae.
        J. Bacteriol. 1969; 98: 637-650
        • Chakraborty P.R.
        • Ruiz-Opazo N.
        • Shouval D.
        • et al.
        Identification of integrated hepatitis B virus DNA and expression of viral RNA in an HBsAg-producing human hepatocellular carcinoma cell.
        Nature. 1980; 286: 531-533
        • Chou S.
        • Merigan T.C.
        Rapid detection and quantitation of human cytomegalovirus in urine through DNA hybridization.
        N. Engl. J. Med. 1983; 308: 921-925
        • Denhardt D.T.
        A membrane filter technique for the detection of complementary DNA.
        Biochem. Biophys. Res. Commun. 1966; 23: 641-646
        • Edman J.C.
        • Gray P.
        • Valenzuela P.
        • et al.
        Integration of hepatitis B virus sequences and their expression in a human hepatoma cell.
        Nature. 1980; 286: 535-537
        • Efstratiadis A.
        • Villa-Komaroff L.
        Cloning of double-stranded DNA.
        in: Stelow J.K. Hollander A. Genetic Engineering. Volume 1. Plenum Press, New York1979
        • Elwell L.P.
        • de Graaff J.
        • Seibert D.
        • et al.
        Plasmid-linked ampicillin resistance in Haemophilus influenzae type B.
        Infect. Immun. 1975; 12: 404-410
        • Gall J.
        • Pardue M.L.
        Nucleic acid hybridization in cytological preparation.
        Methods Enzymol. 1971; 21: 470-480
        • Gillespie D.
        • Spiegelman S.
        A quantitative assay for DNA-RNA hybridization with DNA immobilized on a membrane.
        J. Mol. Biol. 1965; 12: 829-842
        • Grunstein M.
        • Hogness D.
        Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene.
        Proc. Natl. Acad. Sci. U.S.A. 1975; 72: 3961-3966
        • Heffron F.
        • Sublett R.
        • Jacob H.A.
        • et al.
        Origin of the TEM beta-lactamase gene found in plasmids.
        J. Bacteriol. 1975; 122: 250-256
        • Heffron F.
        • Rubers C.
        • Falkow S.
        Transposition of a plasmid DNA sequence which mediates ampicillin resistance: General description and epidemiologic consideration.
        in: Burhari A.I. Shapiro J.A. Adhya S.L. DNA Insertion Elements, Plasmids and Episomes. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York1977
        • Hyypia T.
        • Stalhandske P.
        • Vainionpaa R.
        • et al.
        Detection of enterovirus by spot hybridization.
        J. Clin. Microbiol. 1984; 19: 436-438
        • Inselburg J.
        Isolation, mapping, and examination of effects of TnA insertions in Col El plasmids.
        J. Bacteriol. 1977; 129: 482-491
        • Kafatos F.C.
        • Jones C.W.
        • Efstratiadis A.
        Determination of nucleic acid sequence homologies and relative concentration by a dot hybridization procedure.
        Nucleic Acids Res. 1979; 7: 1541-1552
        • Kingsbury D.T.
        Deoxyribonucleic acid homologies among species of the genus Neisseria.
        J. Bacteriol. 1967; 94: 870-874
        • Langer-Safer P.R.
        • Levine M.
        • Ward D.
        Enzymatic synthesis of biotin-labeled polynucleotides: Novel nucleic acid affinity probes.
        Proc. Natl. Acad. Sci. U.S.A. 1981; 78: 6633-6637
        • Lanfs R.
        • Kaulfers P.-M.
        • Jahn G.
        • et al.
        Molecular characterization of a small Haemophilus influenzae plasmid specifying beta-lactamase and its relationship to R-factor from Neisseria gonorrhoeae.
        J. Gen. Microbiol. 1979; 11: 223-231
        • Marmur J.S.
        • Falkow S.
        • Mandel M.
        New approaches to bacterial taxonomy.
        Annu. Rev. Microbiol. 1963; 18: 329-372
        • Marmur J.
        • Rownd R.
        • Schildkraut C.L.
        Denaturation and renaturation of deoxyribonucleic acid.
        Prog. Nucleic Acids Res. 1963; 1: 231
        • Mayer L.W.
        • Holmes K.K.
        • Falkow S.
        Characterization of plasmid deoxyribo nucleic acid from Neisseria gonorrhoeae.
        Infect. Immun. 1974; 10: 712-717
        • Moseley S.L.
        • Falkow S.
        Nucleotide sequence homology between heat-labile enterotoxin gene of Escherichia coli and Vibrio cholerae deoxyribonucleic acid.
        J. Bacteriol. 1980; 144: 444-446
        • Moseley S.L.
        • Hug I.
        • Alim A.R.M.A.
        • et al.
        Detection of enterotoxigenic Escherichia coli by DNA colony hybridization.
        J. Infect. Dis. 1981; 142: 892-898
        • O’Callaghan C.H.
        • Morris A.
        • Kirby S.M.
        • et al.
        Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.
        Antimicrob. Agents Chemother. 1972; 1: 283-288

      32. Palmer, L., and Falkow, S.: Characterization of Chlamydia trachomatis plasmid DNA. Infect. Immun, (in press).

        • Renz M.
        • Kurz C.
        A colorimetric method for DNA hybridization.
        Nucleic Acids Res. 1984; 12: 3435-3444
        • Rigby P.W.J.
        • Dieckmann M.
        • Rhodes C.
        • et al.
        Labelling deoxyribonucleic acid to high specificity by nick translation with DNA polymerase I.
        J. Mol. Biol. 1977; 113: 237-257
        • Roberts M.
        • Elwell L.P.
        • Falkow S.
        Molecular characterization of two beta-lactamase specifying plasmids isolated from Neisseria gonorrhoeae.
        J. Bacteriol. 1977; 131: 557-563
        • Scotto J.
        • Hadchouel M.
        • Hery C.
        • et al.
        Detection of hepatitis B virus DNA in serum by a simple spot hybridization technique: Comparison with results for other viral markers.
        Hepatology. 1983; 3: 279-284
        • Southern E.M.
        Detection of specific sequences among DNA fragments separated by gel electrophoresis.
        J. Mol. Biol. 1975; 98: 503-517
        • Taub F.
        • Thompson E.B.
        An improved method for preparing large assays of bacterial colonies containing plasmids for hybridization: In situ purification and stable binding of DNA on paper filters.
        Anal. Biochem. 1982; 126: 222-230

      Article info

      Footnotes

      The National Institute of Health provided support for part of this’ research under Small Business Research grant No. 1 R43 AI 20837-01.

      Identification

      DOI: https://doi.org/10.1016/S0272-2712(18)30853-9

      Copyright

      © 1985 Elsevier Inc. Published by Elsevier Inc. All rights reserved.

      ScienceDirect

      Access this article on ScienceDirect
      We use cookies to help provide and enhance our service and tailor content. To update your cookie settings, please visit the for this site.
      Copyright © 2023 Elsevier Inc. except certain content provided by third parties. The content on this site is intended for healthcare professionals.


      RELX