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Research Article| Volume 6, ISSUE 3, P427-440, September 1986

Potential Problems in Serum Protein Electrophoresis

DOI:https://doi.org/10.1016/S0272-2712(18)30791-1
Potential Problems in Serum Protein Electrophoresis
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      Potential problems are described that one could encounter in carrying out an electrophoretic procedure including its ancillary phases of visualization (staining) and quantification (densitometry). Endpoint-like measurements for separated isoenzymes may provide artifactual kinetic values as well, because stain measurement is fixed at a single time whereas reagent blanking in the electrophoretic medium is substituted for the conventional serum initial absorbance readings of test-tube determinations. Truncation of separated electrophoretic zones or opacity of an electrophoretic anticonvection medium such as uncleared cellulose acetate may also interfere with absolute quantification procedures.
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      DOI: https://doi.org/10.1016/S0272-2712(18)30791-1

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      © 1986 Elsevier Inc. Published by Elsevier Inc. All rights reserved.

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