Advertisement
Research Article| Volume 7, ISSUE 3, P655-668, September 1987

Download started.

Ok

Detection of Androgenic Anabolic Steroids in Urine

  • Caroline K. Hatton
    Correspondence
    Corresponding author: Paul Ziffren Olympic Analytical Laboratory, Department of Pharmacology, UCLA Medical Center, Los Angeles, California 90024
    Affiliations
    Associate Director, Paul Ziffren Olympic Analytical Laboratory, and Assistant Research Pharmacologist, University of California, Los Angeles
    Search for articles by this author
  • Don H. Catlin
    Affiliations
    Associate Professor, Departments of Medicine and Pharmacology; University of California, Los Angeles

    Director, Paul Ziffren Olympic Analytical Laboratory; University of California, Los Angeles

    Chief, Division of Clinical Pharmacology, University of California, Los Angeles
    Search for articles by this author
DOI:https://doi.org/10.1016/S0272-2712(18)30737-6
Detection of Androgenic Anabolic Steroids in Urine
Previous ArticleLaboratory Detection of Cannabinoids
Next ArticleCyclosporine Controversies
      This paper is only available as a PDF. To read, Please Download here.
      Virtually all professional and amateur sporting organizations forbid the use of androgenic anabolic steroids (AAS). The International Olympic Committee, United States Olympic Committee, and more recently the National Collegiate Athletic Association enforce the regulations by conducting urine testing. Over the past 3 years we have conducted about 8000 tests for AAS in urine and discovered several hundred positive cases. AAS when present in urine can be detected by screening and confirmatory tests using gas chromatography-mass spectrometry.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribers receive full online access to your subscription and archive of back issues up to and including 2002.

      Content published before 2002 is available via pay-per-view purchase only.

      Subscribe:

      Subscribe to Clinics in Laboratory Medicine
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Register: Create an account
      Institutional Access: Sign in to ScienceDirect

      References

        • Beckett A.H.
        • Cowan D.A.
        Misuse of drugs in sport.
        Br J Sports Med. 1979; 12: 185-194
        • Beckett A.H.
        Use and abuse of drugs in sport.
        J Biosoc Sci (Suppi). 1981; 7: 163-170
        • Beikien L.
        • Schurmeyer T.
        • Hano R.
        • et al.
        Pharmacokinetics of 19-nortestosterone esters in normal men.
        J Steroid Biochem. 1985; 22: 623-629
        • Bergman R.
        • Leach R.E.
        The use and abuse of anabolic steroids in Olympic-caliber athletes.
        Clin Orthop. 1985; 198: 169-172
        • Bertrand M.
        • Masse R.
        • Dugal R.
        GC-MS approach for the detection and characterization of anabolic steroids and their metabolites in biological fluids at major international sporting events.
        Farmaceutische Tijdschrift Belgie. 1978; 3: 85-101
        • Bjorkhem I.
        • Ek H.
        Detection and quantitation of 3-hydroxy-1-methylen-5-androstan-17-one, the major urinary metabolite of methenolone (Primobolan) by isotope dilution-mass spectrometry.
        J Steroid Biochem. 1983; 18: 481-487
        • Bjorkhem I.
        • Ek H.
        Detection and quantitation of 19-norandrosterone in urine by isotope dilution-mass spectrometry.
        J Steroid Biochem. 1982; 17: 447-451
        • Bjorkhem I.
        • Lantto O.
        • Lof A.
        Detection and quantitation of methandienone (Dianabol) in urine by isotope dilution-mass spectrometry.
        J Steroid Biochem. 1983; 18: 481-487
        • Bradlow H.L.
        The hydrolysis of steroid conjugates.
        in: Bernstein S. Solomon S. Chemical and Biological Aspects of Steroid Conjugation. Springer-Verlag, Berlin1970: 131-180
        • Brooks R.V.
        • Firth R.G.
        • Sumner N.A.
        Detection of anabolic steroids by radioimmunoassay.
        Br J Sport Med. 1975; 9: 89-92
        • Brooks R.V.
        • Jeremiah G.
        • Webb W.A.
        • et al.
        Detection of anabolic steroid administration to athletes.
        J Steroid Biochem. 1979; 11: 913-917

      12. Brooks RV, Kicman AT, Nanjee NSE, et al: Detection of the administration of testosterone. In Proc 12th Int Congr Clin Chem, Rio de Janeiro, 1984

        • Burlingame A.L.
        • Whitney J.O.
        Mass spectrometry.
        Anal Chem. 1984; 56: 417R-464R
        • Cartoni G.P.
        • Ciardi M.
        • Giarrusso A.
        • et al.
        Capillary gas chromatographic-mass spectrometric detection of anabolic steroids.
        J Chromatogr. 1983; 279: 515-522
        • Catlin D.H.
        • Kammerer R.C.
        • Hatton C.K.
        • et al.
        Analytical chemistry at the Games of the XXIIIrd Olympiad in Los Angeles, 1984.
        Clin Chem. 1987; 33: 319-327
        • de Merode
        • Prince A.
        Doping tests at the Olympic Games in 1976.
        J Sports Med. 1979; 19: 91-96
        • Donike M.
        • Barwald K.R.
        • Klostermann K.
        • et al.
        Nachweis von exogenem Testosteron.
        in: Sport: Leistung und Gesundheit. Deutscher Arzte-Verlag, Köln1982: 293-300
        • Donike M.
        Acylierung mit bis (Acylamiden), N-methyl-bis (Trifluoracetamid) und bis (Trifluoracetamid), zwei neue Reagen zien zur Trifluoracetylierung.
        J Chromatogr. 1973; 78: 237-241
        • Donike M.
        • Zimmermann J.
        • Barwald K-R
        • et al.
        Routinebestimmung von Anabolike im Ham.
        Deutsche Z Sportmed. 1984; 35: 14-24
        • Donike M.
        • Zimmermann J.
        Zur Darstellung von Trimethylsilyl-, Triethylsilyl-, und tert-Butyldimethylsilyl-enolathern von Ketosteroiden fur gas-chromatographische und mas- senspektrome-trische Untersuchungen.
        J Chromatogr. 1980; 202: 483-486
        • Dugal R.
        • Dupuis C.
        • Bertrand M.J.
        Radioimmunoassay of anabolic steroids: An evaluation of three antisera for the detection of anabolic steroids in biological fluids.
        Br J Sport Med. 1977; 1: 162-169
        • Durbeck H.W.
        • Buker I.
        Studies on anabolic steroids. The mass spectra of 11-methyl-17-hydroxy-1, 4-androstadien-3-one (Dianabol) and its metabolites.
        Biomed Mass Spectrom. 1980; 7: 437-445
        • Durbeck H.W.
        • Buker I.
        • Scheulen B.
        • et al.
        Gas chromatographic and capillary column gas chromatographic—mass spectrometric determination of synthetic anabolic steroids.
        J Chromatogr. 1978; 167: 117-124
        • Frischkorn C.G.B.
        • Frischkorn H.E.
        Investigations of anabolic drug abuse in athletics and cattle feed. II. Specific determination of methandienone (Dianabol) in urine in nanogram amounts.
        J Chromatogr. 1978; 151: 331-338
        • Gaskeil S.J.
        Analysis of steroids by mass spectrometry.
        Methods Biochem Anal. 1983; 29: 385-434
        • Heftmann E.
        • Lin J-T
        Steroid analysis by high-performance liquid chromatography.
        J Liquid Chromatogr. 1982; 5: 121-173
        • Lantto O.
        • Bjorkhem I.
        • Ek H.
        • et al.
        Detection and quantitation of stanozolol (Stromba) in urine by isotope dilution-mass fragmentography.
        J Steroid Biochem. 1981; 14: 721-727
        • Oehrle K.L.
        • Vogt K.
        • Hoffmann B.
        Determination of trenbolone and trenbolone acetate by thin-layer chromatography in combination with a fluorescence colour reaction.
        J Chromatogr. 1975; 114: 244-246
        • Orme M L’E
        • Back D.J.
        • Breckenridge A.M.
        Clinical pharmacokinetics of oral contraceptive steroids.
        Clin Pharmcokinet. 1983; 8: 95-136
        • Ryan A.J.
        Anabolic steroids are fool’s gold.
        Proc. 1981; 40: 2682-2688
        • Uralets V.P.
        • Semenova V.A.
        • Yakushin M.A.
        • et al.
        Analysis of anabolic steroids in body fluids by capillary gas chromatography with a two-channel detection system and a computer.
        J Chromatogr. 1983; 279: 695-707
        • Vanluchene E.
        • Sandra P.
        Analysis of steroid hormones.
        in: Jennings W.J.G. Applications of Glass Capillary Gas Chromatography. Marcel Dekker, New York1981
        • Wade N.
        Anabolic steroids: Doctors denounce them, but athletes aren’t listening (editorial).
        Science. 1972; 176: 1399-1403
        • Ziegler J.B.
        Introduction.
        in: Goldman R. Death in the Locker Room. Icarus Press, South Bend, Indiana1984

      Article info

      Identification

      DOI: https://doi.org/10.1016/S0272-2712(18)30737-6

      Copyright

      © 1987 Elsevier Inc. Published by Elsevier Inc. All rights reserved.

      ScienceDirect

      Access this article on ScienceDirect

      The content on this site is intended for healthcare professionals.


      We use cookies to help provide and enhance our service and tailor content. To update your cookie settings, please visit the Cookie Preference Center for this site.
      Copyright © 2023 Elsevier Inc. except certain content provided by third parties.


      RELX