Research Article| Volume 9, ISSUE 1, P169-186, March 1989

Triglyceride Measurement and Its Relationship to Heart Disease

      This paper is only available as a PDF. To read, Please Download here.
      The role of trigylcerides in atherosclerosis remains controversial, although it may be important in certain population subgroups. The triglyceride-rich lipoproteins play an important role in lipid metabolism. Measurement of plasma triglycerides is important for routine lipid-ather-osclerosis risk assignment. Significantly more attention needs to be focused on standardization of triglyceride measurements in clinical laboratories.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribers receive full online access to your subscription and archive of back issues up to and including 2002.

      Content published before 2002 is available via pay-per-view purchase only.


      Subscribe to Clinics in Laboratory Medicine
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Ahmed S.
        • Lippel K.
        • Bachorik P.
        • et al.
        External quality control survey of triglyceride (triacylglycerol) analyses performed by 12 lipid research clinics.
        Clin Chem. 1979; 25: 880
        • Albrink M.J.
        • Man E.B.
        Serum-triglycerides in coronary artery disease.
        Arch Intern Med. 1959; 103: 4
        • Antonis A.
        • Bersohn I.
        Serum-triglyceride levels in South African Europeans and Bantu and in ischaemic heart disease.
        Lancet. 1960; 1: 998
        • Ballantyne D.
        • Lawrie T.D.V.
        Hyperlipoproteinemia and peripheral vascular disease.
        Clin Chim Acta. 1973; 47: 269
        • Beyrer K.
        • May R.
        • Sailer S.
        Blutilipide und Glukosetoleranz bei 100 Patienten mit arteriosklerotischem Verschouss anden unteren Extremitaten.
        Wien Klin Wo-chenschr. 1968; 80: 392
        • Bonderman D.P.
        • Proksch G.
        • Bonderman P.W.
        Addition of triglyceride to serum for use in quality control and reference.
        Clin Chem. 1976; 22: 1299
        • Bradley W.A.
        • Gianturco S.H.
        Low density lipoprotein receptor-mediated interactions of triglyceride (TG)-rich lipoproteins.
        in: Fidge N.H. Nestel P.J.S. Atherosclerosis. ed 7. Elsevier Science Publishers, Amsterdam1986: 217
        • Brunzell J.D.
        • Schrott H.G.
        • Motulsky A.G.
        • et al.
        Myocardial infarction in the familial forms of hypertriglyceridemia.
        Metabolism. 1976; 25: 313
        • Bucolo G.
        • David H.
        Quantitative determination of serum triglycerides by the use of enzymes.
        Clin Chem. 1973; 19: 476
        • Cambien F.
        • Jacqueson A.
        • Richard J.L.
        • et al.
        Is the level of serum triglyceride a significant predictor of coronary death in “normocholesterolemic” subjects? The Paris Prospective Study.
        Am J Epidemiol. 1986; 124: 624
        • Canner P.L.
        • Berge K.G.
        • Wenger N.K.
        • et al.
        Fifteen-year mortality in coronary drug project patients: Long-term benefit with niacin.
        J Am Coll Cardiol. 1986; 8: 1245
        • Carlson L.A.
        • Bottiger L.E.
        Risk factors for ischaemic heart disease in men and women.
        Acta Med Scand. 1985; 218: 207
        • Carlson L.A.
        • Bottiger L.E.
        • Ahfeldt P.E.
        Risk factors for myocardial infarction in the Stockholm Prospective Study: A 14-year follow-up focusing on the role of plasma triglycerides and cholesterol.
        Acta Med Scand. 1979; 206: 351
        • Carlson L.A.
        Determination of serum triglycerides.
        J Atherosclerosis Res. 1963; 3: 334
        • Castelli W.P.
        The triglyceride issue: A view from Framingham.
        Am Heart J. 1984; 112: 432
        • Cheung C.K.
        Effect of detergent on triglyceride assay.
        Clin Chem. 1987; 33: 202
        • Chong-Kit R.
        • McLaughlin P.
        Fully automated, all-enzymatic triglyceride method adapted to the Gemsaec centrifugal analyzer, with use of an aequeous triolein standard.
        Clin Chem. 1974; 20: 1454
        • College of American Pathologists
        Comprehensive Set C-A Comprehensive Chemistry Survey.
        Participant Survey. College of American Pathologists, Skokie, Ill1988
        • Committee of Principal Investigators
        A cooperative trial in the primary prevention of ischemic heart disease using clofibrate.
        Br Heart J. 1978; 40: 1069
        • Consensus Development Conference
        Lowering of cholesterol to prevent heart disease.
        JAMA. 1985; 253: 2080
        • Consensus Development Conference
        Treatment of hypertriglyceridemia.
        JAMA. 1984; 251: 1196
        • Cox D.R.
        • Oakes D.
        Analysis of survival data.
        Monographs on Statistics and Applied Probability. Chapman & Hall, Ltd, London1984
        • Davingnon J.
        • Lussier-Cacan S.
        • Ortin-George M.
        • et al.
        Plasma lipids and lipoprotein patterns in angiographically graded atherosclerosis of the legs and in coronary heart disease.
        Can Med Assoc J. 1977; 116: 1245
        • Delong D.V.
        • DeLong E.R.
        • Wood P.D.
        • et al.
        A comparison of methods for the estimation of plasma low- and very low-density lipoprotein cholesterol: The Lipid Research Clinics Prevalence Study.
        JAMA. 1986; 256: 2372
        • Duffield R.G.M.
        • Lewis B.
        • Miller N.E.
        • et al.
        Treatment of hyperlipidemia retards progression of symptomatic femoral atherosclerosis: A randomized controlled trial.
        Lancet. 1983; 2: 639
        • The Expert Panel
        Report of the National Cholesterol Education Program′s Expert Panel on detection, evaluation, and treatment of high blood cholesterol in adults.
        Arch Intern Med. 1988; 148: 36
        • Fager G.
        • Wiklund
        • Olofsson S.O.
        • et al.
        Multivariate analyses of serum apolipoproteins and risk factors in relation to acute myocardial infarction.
        Arteriosclerosis. 1981; 1: 273
        • Fossati P.
        • Prencipe L.
        Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide.
        Clin Chem. 1982; 28: 2077
        • Frick M.H.
        • Elo O.
        • Haapa K.
        • et al.
        Helsinki Heart Study: Primary prevention trial with gemfibrozil in middle-aged men with dyslipidemia.
        N Engl J Med. 1987; 317: 1237
        • Friedewald W.T.
        • Levy R.I.
        • Fredrickson D.S.
        Estimation of the concentration of low-density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge.
        Clin Chem. 1972; 18: 499
        • Greenhalgh R.M.
        • Lewis B.
        • Rosengarten D.S.
        • et al.
        Serum lipids and lipoproteins in peripheral vascular disease.
        Lancet. 1971; 2: 947
        • Greenhalgh R.M.
        • Taylor G.W.
        • Kaye J.
        A comparison of fasting lipid concentrations and lipoprotein patterns in patients with stenosing and dilating forms of peripheral arterial disease.
        J Cardiovasc Surg (Torino). 1975; 16: 150
        • Guilmet R.
        • Altaffer M.
        • Statland B.
        • et al.
        Falsely high results for triglycerides in patients receiving intravenous nitroglycerin.
        Clin Chem. 1986; 32: 2098
        • Havel R.J.
        • Eder H.A.
        • Bragdon J.H.
        Distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum.
        J Clin Invest. 1955; 34: 1345
        • Hulley S.B.
        • Rosenman R.H.
        • Bawol R.D.
        • et al.
        Epidemiology as a guide to clinical decisions: The association between triglyceride and coronary heart-disease.
        N Engl J Med. 1980; 302: 1383
        • Kannel W.B.
        • Garcia M.J.
        • McNamara P.M.
        • et al.
        Serum lipid precursors of coronary artery disease.
        Hum Pathol. 1971; 2: 129
        • Kaukola S.
        • Manninen V.
        • Halonen P.I.
        Serum lipids with special reference to HDL cholesterol and triglycerides in young male survivors of acute myocardial infarction.
        Acta Med Scand. 1980; 208: 41
        • Kessler G.
        • Lederer H.
        Fluorometric measurement of triglycerides.
        in: Skeggs L.T. Automation in Analytical Chemistry. Technicon Symposia 1965. Mediad, New York1966: 341
        • Kuckmak M.
        • Taylor L.
        • Olansky A.S.
        Suitability of frozen and lyophilized reference sera for cholesterol and triglyceride determinations.
        Clin Chem Acta. 1982; 120: 261
        • Kukita H.
        • Imamura Y.
        • Hamada M.
        • et al.
        Plasma lipids and lipoproteins in Japanese male patients with coronary artery disease and in their relatives.
        Atherosclerosis. 1982; 42: 21
        • Lipid Research Clinics Program Epidemiology Committee
        Plasma lipid distributions in selected North American population. The Lipid Research Clinics Program Prevalence Study.
        Circulation. 1979; 60: 427
        • Later R.
        • Pegon Y.
        • Contreras P.
        • et al.
        A fluorescent biological compound that can cause error in enzymic triglycerides determination.
        Clin Chem. 1985; 31: 499
        • Luley C.
        • Prellivitz W.
        Qualitative detection of chylomicrons with a high speed laboratory centrifuge compared with 16-h-standing test, lipoprotein electrophoresis, and preparative ultracentrifugation.
        Clin Chem. 1988; 34: 1362
      1. Manual of Laboratory Operations, Lipid Research Clinics Program: Lipid and lipoprotein analysis. U.S. Department of Health and Human Services, Public Health Service, National Institutes of Health, Publication No. 361-132:678, 1982

        • McNeely S.
        • Seatter K.
        • Yuhaniak J.
        • et al.
        The 16 hr standing test and lipoprotein electrophoresis compared for detection of chylomicrons in plasma.
        Clin Chem. 1981; 27: 731
        • McPherson R.A.
        • Brown K.
        • Agarwai R.
        • et al.
        Hydroxylurea interferes negatively with triglyceride measurement by a glycerol oxidase method.
        Clin Chem. 1985; 31: 1355
        • Newell R.G.
        • Bliss B.P.
        Lipoproteins and the relative importance of plasma cholesterol and triglycerides in peripheral arterial disease.
        Angiology. 1973; 24: 297
        • Noble R.D.
        Electrophoretic separation of plasma lipoproteins in agarose gel.
        J Lipid Res. 1968; 9: 693
        • Rautela G.S.
        • Slater S.
        • Arvan D.A.
        Assessment of the need for triglyceride blank measurements.
        Clin Chem. 1973; 19: 1193
        • Rosenman R.H.
        • Brand R.J.
        • Sholtz R.I.
        • et al.
        Multivariate prediction of coronary heart disease during 8.5 year follow-up in the Western Collaborative Group Study.
        Am J Cardiol. 1976; 37: 903
        • Ryder K.
        • Glick M.
        • Bertram S.
        • et al.
        Effect of dry-skin products on Ektachem triglyceride results.
        Clin Chem. 1986; 32: 1410
        • Seidel D.
        • Alaupovic P.
        • Furman R.
        • et al.
        A lipoprotein characterizing obstructive jaundice. II. Isolation and partial characterization of the protein moieties of low density lipoproteins.
        J Clin Invest. 1970; 49: 2396
        • Shepherd J.
        • Caslake M.J.
        • Lorimer A.R.
        • et al.
        Fenofibrate reduces low density lipoprotein catabolism in hypertriglyceridemic subjects.
        Arteriosclerosis. 1985; 5: 162
        • Sirtori C.R.
        • Biasi G.
        • Vercellio G.
        • et al.
        Diet, lipids and lipoproteins in patients with peripheral vascular disease.
        Am J Med Sci. 1974; 268: 325
        • Slack J.
        Risks of ischemic heart disease in familial hyperlipoproteinemic states.
        Lancet. 1969; 2: 1380
        • Spain M.A.
        • Wu A.H.B.
        Bilirubin interference with determination of uric acid, cholesterol and triglycerides in commercial peroxidase-coupled assay, and the effect of ferrocyanide.
        Clin Chem. 1986; 32: 518
        • Stein E.A.
        Lipid, lipoproteins, and apolipoproteins.
        in: Tietz N.W. Textbook of Clinical Chemistry. WB Saunders Co, Philadelphia1986: 829
        • Stein E.A.
        • McNeely S.
        • Steiner P.
        Electrophoretic separation of high-density lipoprotein cholesterol evaluated and compared with the modified lipid research clinic procedure.
        Clin Chem. 1979; 24: 1934
        • Steiner G.
        Diabetes and atherosclerosis: An overview.
        Diabetes. 1981; 30: 1
        • Stokes Y.M.
        • Salmond C.E.
        • Carpenter L.M.
        • et al.
        Stability of total cholesterol, high-den-sity-lipoprotein cholesterol, and triglycerides in frozen sera.
        Clin Chem. 1986; 32: 995
        • Sullivan D.R.
        • Kruijswijk Z.
        • West C.E.
        • et al.
        Determination of serum triglycerides by an accurate enzymatic method not affected by free glycerol.
        Clin Chem. 1985; 31: 1227
        • Tan M.H.
        • Macintosh W.
        • Weldon K.L.
        • et al.
        Serum high density lipoprotein cholesterol in patients with abnormal coronary arteries.
        Atherosclerosis. 1980; 37: 187
        • Tiedink G.M.
        • Katan M.B.
        Frozen storage of serum does not affect cholesterol and triglyceride concentration in lipoproteins as separated by gradient ultracentrifugation.
        Clin Chem. 1988; 34: 593
        • VanHandel E.
        • Zilversmit J.
        Micromethod for the direct determination of serum triglycerides.
        J Lab Clin Med. 1957; 50: 152
        • Walldius G.
        Plasma triglycerides and the development of atherosclerotic disease.
        in: Fidge N.H. Nestel P.J. Atherosclerosis. ed 7. Elsevier Science Publishers, Amsterdam1986: 205
        • Whayne T.F.
        • Alaupovic P.
        • Curry M.D.
        • et al.
        Plasma apolipoprotein B and VLDL-, LDL-, and HDL-cholesterol as risk factors in the development of coronary artery disease in male patients examined by angiography.
        Atherosclerosis. 1981; 39: 411
        • Zilversmit D.B.
        A proposal linking atherogenesis to the interaction of endothelial lipoprotein lipase with triglyceride-rich lipoproteins.
        Circ Res. 1973; 33: 633
        • Zoppi F.
        Single-cuvet sequential determination of triglyceride and cholesterol.
        Clin Chem. 1985; 31: 2036