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Research Article| Volume 9, ISSUE 1, P61-72, March 1989

Measurement of Total Cholesterol, HDL-Cholesterol, and LDL-Cholesterol

  • Paul S. Bachorik
    Correspondence
    Corresponding author: The Children′s Medical and Surgical Center, 604, The Johns Hopkins Hospital, Baltimore, Maryland 21205.
    Affiliations
    Associate Professor, Departments of Pediatrics and Laboratory Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland
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DOI:https://doi.org/10.1016/S0272-2712(18)30642-5
Measurement of Total Cholesterol, HDL-Cholesterol, and LDL-Cholesterol
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      Plasma concentrations of LDL and HDL are generally measured in terms of their cholesterol content. This requires the separation of the appropriate lipoprotein-containing fractions. The separation and measurement of each fraction are described.
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      References

        • Abell L.L.
        • Levy B.B.
        • Brodie B.B.
        • et al.
        A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity.
        J Biol Chem. 1952; 195: 357
        • Albers J.J.
        • Adolphson J.L.
        • Hazzard W.R.
        Radioimmunoassay of human plasma Lp(a) lipoprotein.
        J Lipid Res. 1977; 18: 331
        • Bachorik P.S.
        Collection of blood samples for lipoprotein analysis.
        in: Cooper G.R. Selected Methods of Clinical Chemistry. 10. Am Assoc for Clin Chem, Washington, DC1983: 87
        • Bachorik P.S.
        • Albers J.J.
        Precipitation methods for quantification of lipoproteins.
        Methods Enzymol. 1986; 129: 78
        • Bachorik P.S.
        • Most B.
        • Lippel K.
        • et al.
        Plasma lipoprotein analysis: Relative precision of total cholesterol and lipoprotein cholesterol measurements in twelve Lipid Research Clinics Laboratories.
        Clin Chem. 1981; 27: 1217
        • Bachorik P.S.
        • Walker R.
        • Brownell K.D.
        • et al.
        Determination of HDL-cholesterol levels in stored human plasma samples.
        J Lipid Res. 1980; 21: 608
        • Bachorik P.S.
        • Walker R.
        • Kwiterovich Jr., P.O.
        Determination of high density lipoprotein cholesterol in human plasma stored at—70°C.
        J Lipid Res. 1982; 23: 1236
        • Brown M.S.
        • Goldstein J.L.
        • Fredrickson D.S.
        Familial type 3 hyperlipoproteinemia (dys-betalipoproteinemia).
        in: Stanbury J.B. Wyngaarden J.B. Fredrickson D.S. Goldstein J.L. Brown M.S. The Metabolic Basis of Inherited Disease. 5th ed. McGraw-Hill, New York1983: 655
        • Burstein M.
        • Legmann P.
        Clarkson T.B. Kritchevsky D. Pollak O.J. Monographs on Atherosclerosis. Karger, New York1982
        • Cheung M.C.
        Characterization and distribution of HDL subpopulations.
        in: Lippel K. Proceedings of the Workshop on Lipoprotein Heterogeneity. Rockville, Maryland1987: 341 (NIH Publication No. 87-2646)
        • DeLong D.M.
        • DeLong E.R.
        • Wood P.D.
        • et al.
        A comparison of methods for the estimation of plasma low- and very low-density lipoprotein cholesterol. The Lipid Research Clinics Prevalence Study.
        JAMA. 1986; 256: 2372
        • Friedewald W.T.
        • Levy R.I.
        • Fredrickson D.S.
        Estimation of the concentration of low density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge.
        Clin Chem. 1972; 18: 499
        • Goldstein J.L.
        • Brown M.S.
        Familial hypercholesterolemia.
        in: Stanbury J.B. Wyngaarden J.B. Fredrickson D.S. Goldstein J.L. Brown M.S. The Metabolic Basis of Inherited Disease. 5th ed. McGraw-Hill, New York1983: 672
        • Laboratory Methods Committee, LRC Program
        Cholesterol and triglyceride concentrations in serum-plasma pairs.
        Clin Chem. 1977; 23: 60
        • Lindgren F.T.
        • Jensen L.C.
        The isolation and quantitative analysis of serum lipoproteins.
        in: Nelson G.J. Blood Lipids and Lipoproteins: Quantitation, Composition and Metabolism. Wiley-Interscience, New York1972: 181
        • Lipid Research Clinics Program: 1974
        Manual of Laboratory Operations. 1: Lipid and Lipoprotein Analysis.
        DHEW Publication No. (NIH) 75-628. 1982; (Revised version, October)
        • Murai A.
        • Miyahara T.
        • Fujimoto N.
        • et al.
        Lp(a) lipoprotein as a risk factor for coronary heart disease and cerebral infarction.
        Atherosclerosis. 1986; 59: 199
        • Nikkila E.A.
        Familial lipoprotein lipase deficiency and related disorders of chylomicron metabolism.
        in: Stanbury J.B. Wyngaarden J.B. Fredrickson D.S. Goldstein J.L. Brown M.S. The Metabolic Basis of Inherited Disease. 5th ed. McGraw-Hill, New York1983: 622
        • Scanu A.M.
        • Byrne R.E.
        • Mihovilovic M.
        Functional roles of plasma high density lipoproteins.
        CRC Critical Rev Biochem. 1982; 13: 109
        • Segal P.
        • Bachorik P.S.
        • Rifkind B.
        • et al.
        Lipids and dyslipoproteinemia.
        in: Henry J.B. 17th ed. Clinical Diagnosis and Management by Laboratory Methods. WB Saunders, Philadelphia1984: 181

      21. Segrest JP, Ray MJ: HDL subspecies: Structural basis and relationship to atherosclerosis. InLippel K (ed): Proceedings of the Workshop on Lipoprotein Heterogeneity Maryland, Rockville, NIH Publication No. 87-2646, 1987, p 351

      Article info

      Footnotes

      This work was supported in part by NIH grant 5R01 HL31450.

      Identification

      DOI: https://doi.org/10.1016/S0272-2712(18)30642-5

      Copyright

      © 1989 Elsevier Inc. Published by Elsevier Inc. All rights reserved.

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