Advertisement
Research Article| Volume 10, ISSUE 1, P215-236, March 1990

In Situ Hybridization

  • Mark H. Stoler
    Correspondence
    Address reprint requests to: Mark H. Stoler, MD, Department of Pathology/L25, The Cleveland Clinic Foundation, One Clinic Center, 9500 Euclid Ave. Cleveland, OH 44195-5138
    Affiliations
    Staff Pathologist, and Director, Molecular Biology Laboratory, Department of Pathology, The Cleveland Clinic Foundation, Cleveland, Ohio.
    Search for articles by this author
DOI:https://doi.org/10.1016/S0272-2712(18)30589-4
In Situ Hybridization
Previous ArticlePlastic Section Immunohistochemistry in the Diagnosis of Hematopoietic and Lymphoid Neoplasms
Next ArticleContributing Editors Comments
      This paper is only available as a PDF. To read, Please Download here.
      In situ hybridization is a technique with wide application in laboratory medicine. In this article, the basic and technical aspects of in situ hybridization are reviewed, and applications are discussed that emphasize methods for the detection of gene expression.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribers receive full online access to your subscription and archive of back issues up to and including 2002.

      Content published before 2002 is available via pay-per-view purchase only.

      Subscribe:

      Subscribe to Clinics in Laboratory Medicine
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Register: Create an account
      Institutional Access: Sign in to ScienceDirect

      References

        • Angerer L.M.
        • Angerer R.C.
        Detection of poly A+ RNA in sea urchin eggs and embryos by quantitative in situ hybridization.
        Nucl Acids Res. 1981; 9: 2819-2840
        • Angerer L.M.
        • Stoler M.H.
        • Angerer R.C.
        In situ hybridization with RNA probes: An annotated recipe.
        In Situ Hybridization: Applications to Neurobiology. Oxford University Press, New York1987: 42-70 (This is a detailed annotated recipe on how to do in situ hybridization. This same monograph contains several other protocols by other groups and is an up-to-date reference on the state of the art in this field)
        • Angerer R.C.
        • Cox K.H.
        • Angerer L.M.
        In situ hybridization for cellular RNAs.
        Gen Eng. 1985; 7: 43-65
        • Brahic M.
        • Hasse A.T.
        Detection of viral sequences of low iteration frequency by in situhybridization.
        Proc Natl Acad Sci. 1978; 81: 5545-5548
        • Cox K.H.
        • DeLeon D.V.
        • Angerer L.M.
        • et al.
        Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes.
        Dev Biol. 1984; 87: 308-318
        • DeLellis R.A.
        • Wolfe H.J.
        New techniques in gene product analysis.
        Arch Pathol Lab Med. 1987; 111: 620-627
        • Edberg S.C.
        Nucleic acid hybridization analysis to elucidate microbial pathogens.
        Lab Med. 1986; 17: 735
        • Feinberg A.P.
        • Vogelstein B.
        A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity.
        Anal Biochem. 1983; 132: 6-13
        • Fenoglio-Preiser C.M.
        • Willman C.L.
        Molecular biology and the pathologist.
        Arch PatholLab Med. 1987; 111: 601-614
        • Gall J.G.
        • Pardue M.
        Formation and detection of RNA-DNA hybrid molecules in cytological preparations.
        PNAS. 1969; 63: 378-383
        • Grady-Leopardi E.F.
        • et al.
        Detection of N-myc oncogene expression in human neuroblastoma by in situ hybridization and blot analysis: Relationship to clinical outcome.
        Cancer Res. 1986; 46: 3196-3199
        • Grody W.W.
        • Cheng L.
        • Lewin K.J.
        Application of in situ DNA hybridization technology to diagnostic surgical pathology.
        Pathol Annu. 1987; 22: 151
        • Kreig P.A.
        • Rebagliati M.R.
        • Green M.R.
        • et al.
        Synthesis of hybridization probes and RNA substrates with SP6 RNA polymerase.
        Gen Eng. 1985; 7: 165-184
        • Lloyd R.V.
        Use of molecular probes in the study of endocrine diseases.
        Hum Pathol. 1987; 18: 1199-1211
        • Melton D.
        • Kreig P.
        • Rebagliati M.
        • et al.
        Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes for plasmids containing a bacteriophage SP6 promoter.
        Nucl Acid Res. 1984; 12: 7035-7056
        • Pardue M.L.
        • Dawid I.B.
        Chromosomal location of two DNA segments that flank ribosomal insertion-like sequences in Drosophila: Flanking sequences are mobile elements.
        Chromosoma. 1981; 83: 29-43
        • Rentrop M.
        • et al.
        Aminoalkylsilane-treated glass slides as support for in situ hybridization of keratin c-DNAs to frozen sections under varying fixation and retreatment conditions.
        Histochemical J. 1986; 18 (This is the TES slide coating protocol that also works well for paraffin sections. Also see J Clin Pathol 40:1256-1257, 1987): 271-276
        • Rigby P.W.J.
        • Dieckmann M.
        • Rhodes C.
        • et al.
        Labelling deoxynucleic acid to high-specific activity in vitro by nick-translation with DNA polymerase I.
        J Mol Biol. 1977; 113: 237-251
        • Sklar J.
        DNA hybridization in diagnostic pathology.
        Hum Pathol. 1985; 16: 654
        • Stoler M.H.
        Broker TR: In situ detection of human papillomavirus DNAs and messenger RNAs in genital condylomas and a cervical carcinoma.
        Hum Pathol. 1986; 17: 1250-1259
        • Stoler M.H.
        • Eskin T.A.
        • Benn S.
        • et al.
        Human T-cell lymphotrophic virus type III infection of the central nervous system: A preliminary in situ analysis.
        JAMA. 1986; 256: 2360-2364
        • Stoler M.H.
        • Wolinsky S.M.
        • Whitbeck A.
        • et al.
        Differentiation-linked human papillomavirus type 6 and 11 transcription in genital condylomata revealed by in situ hybridization with message-specific RNA probes.
        Virology. 1989; 172: 331-340
      23. The Journal of Histochemistry and Cytochemistry. 1986; 34 (This is asymposiumissue on ISH and contains several Protocols as well as many references to the original literature): 1

      Article info

      Identification

      DOI: https://doi.org/10.1016/S0272-2712(18)30589-4

      Copyright

      © 1990 Elsevier Inc. Published by Elsevier Inc. All rights reserved.

      ScienceDirect

      Access this article on ScienceDirect

      The content on this site is intended for healthcare professionals.


      We use cookies to help provide and enhance our service and tailor content. To update your cookie settings, please visit the Cookie Preference Center for this site.
      Copyright © 2023 Elsevier Inc. except certain content provided by third parties.


      RELX