Research Article| Volume 14, ISSUE 2, P351-365, June 1994

In Situ Polymerase Chain Reaction and HIV-1

  • Omar Bagasra
    Address reprint requests to Omar Bagasra, MD, PhD, Infectious Diseases Division, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Jefferson Alumni Hall, 1020 Locust Street, Suite 329D, Philadelphia, PA 19107.
    From the Dorrance H. Hamilton Laboratories, Section of Molecular Retro virology, Infectious Disease Division, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania
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  • Roger J. Pomerantz
    From the Dorrance H. Hamilton Laboratories, Section of Molecular Retro virology, Infectious Disease Division, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania
    Search for articles by this author
      This paper is only available as a PDF. To read, Please Download here.
      The study of low-copy viral or genomic DNA sequences by in situ hybridization is often limited by sensitivity. The ability to detect a single copy of a specific gene in situ has many advantages and multiple applications in molecular biology, pathology, and cell biology. One of the limitations of in situ hybridization, however, is detection and quantitation of very low levels of nucleic acid targets where the signal is insufficient to distinguish it clearly from background noise. The advent of polymerase chain reaction technology to amplify target nucleic acids provides an opportunity to develop new technologies to examine this end of the spectrum of gene expression or infection.
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