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Research Article| Volume 14, ISSUE 2, P335-349, June 1994

Clinical Application of Reverse Transcription-Polymerase Chain Reaction for HIV Infection

  • Mark Holodniy
    Correspondence
    Address reprint requests to Mark Holodniy, MD, Infectious Disease Section(111-ID), Department of Veterans Affairs Medical Center, 3801 Miranda Avenue, Palo Alto, CA 94304.
    Affiliations
    From the Division of Infectious Diseases, Department of Veterans Affairs Medical Center, Palo Alto
    Department of Medicine, Stanford University, Stanford, California
    Search for articles by this author
      This paper is only available as a PDF. To read, Please Download here.
      This article describes the current knowledge of the various RT-PCR assays and their clinical application in HIV disease. The importance of assay precision and sample handling is emphasized. A review of quantification techniques is also presented. Finally, the relevant literature describing the application of RT-PCR for both virion RNA in plasma and cellular mRNA is reviewed.
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