Research Article| Volume 16, ISSUE 1, P213-222, March 1996

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Diagnostic Molecular Pathology in the Twenty-first Century

  • Frederick L. Kiechle
    Address reprint requests to: Frederick L. Kiechle, MD, PhD, Department of Clinical Pathology, William Beaumont Hospital, 3601 West Thirteen Mile Road, Royal Oak, MI 48073
    From the Department of Clinical Pathology, William Beaumont Hospital, Royal Oak; and the Department of Pathology, Wayne State University School of Medicine, Detroit, Michigan
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      Diagnostic molecular pathology is expanding rapidly with the aid of the Human Genome Project and the development of potentially user-friendly molecular diagnostic methods. The diagnostic molecular pathology laboratory of the future must be prepared to purify DNA or RNA from a variety of sources and to investigate the sequence of the target genome of interest using automated amplification and hybridization detection systems. There will be a shift in the emphasis from phenotypic to genotypic diagnosis, and the diagnostic molecular pathology laboratory of the early twenty-first century will perform 5% to 10% of the volume of all laboratory testing.
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