Advertisement
Research Article| Volume 16, ISSUE 1, P49-60, March 1996

Download started.

Ok

The Role of Molecular Diagnostics in the Clinical Microbiology Laboratory

  • Vickie S. Baselski
    Correspondence
    Address reprint requests to Vickie S. Baselski, PhD, Department of Pathology, University of Tennessee, 899 Madison Avenue, Memphis, TN 38163
    Affiliations
    From the Department of Pathology, University of Tennessee, Memphis, Tennessee
    Search for articles by this author
DOI:https://doi.org/10.1016/S0272-2712(18)30286-5
The Role of Molecular Diagnostics in the Clinical Microbiology Laboratory
Previous ArticleBcl-2 Gene Rearrangements in Lymphoid Malignancies
Next ArticleMultiplex Polymerase Chain Reaction for the Diagnosis of Sexually Transmitted Diseases
      This paper is only available as a PDF. To read, Please Download here.
      With the exquisite sensitivity, inherent specificity, and versatility of polymerase chain reaction (PCR) and other amplification techniques, it is clear that infectious disease diagnostic tests based on these methods will assume a niche quickly in many clinical microbiology laboratories. Routine implementation, however, requires that many issues be addressed, including control of ampli- con contamination, specimen treatment to avoid inhibitors, interpretation of positive results when clinical significance is unclear, and cost. Laboratory experience with the Chlamydia Amplicor product (Roche Molecular Systems, Branchburg, NJ) is discussed to illustrate these issues. Commercial kit products using nucleic acid amplification techniques can be introduced into a routine laboratory easily and with a high degree of accuracy.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribers receive full online access to your subscription and archive of back issues up to and including 2002.

      Content published before 2002 is available via pay-per-view purchase only.

      Subscribe:

      Subscribe to Clinics in Laboratory Medicine
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Register: Create an account
      Institutional Access: Sign in to ScienceDirect

      References

        • Amicosante M.
        • Richeldi L.
        • Trenti G.
        • et al.
        Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin.
        J Clin Microbiol. 1995; 33: 629
        • An Q.
        • Liu J.
        • O’Brien W.
        • et al.
        Comparison of characteristics of Qß-replicase amplified assay with competitive PCR assay for Chlamydia trachomatis.
        J Clin Microbiol. 1995; 33: 58

      3. Baselski V, Mercer B, Shaw B: Comparison of Roche Chlamydia PCR test to McCoy cell culture in obstetric patients [abstract C-421]. In Abstracts of the 92nd General Meeting of the American Society for Microbiology, New Orleans, 1992, p 490

      4. Baselski V, Mercer B, Robison M, et al: Comparison of Gen-Probe PACE-2 and Roche Amplicor to cell culture for detection of Chlamydia in obstetric patients [abstract C-165]. In Abstracts of the 93rd General Meeting of the American Society for Microbiology, Atlanta, 1993, p 475

        • Bass C.A.
        • Jungkind D.L.
        • Silverman N.S.
        • et al.
        Clinical evaluation of a new polymerase chain reaction assay for detection of Chlamydia trachomatis in endocervical specimens.
        J Clin Microbiol. 1993; 31: 2648
        • Check W.A.
        Tuberculosis: What role for rapid test?.
        CAP Today. 1994; 8: 1
        • Cherian T.
        • Bobo L.
        • Steinhoff M.C.
        • et al.
        Use of PCR-enzyme immunoassay for identification of influenza A virus matrix RNA in clinical samples negative for cultivable virus.
        J Clin Microbiol. 1994; 32: 623
        • Chemesky M.A.
        • Jang D.
        • Lee H.
        • et al.
        Diagnosis of Chlamydia trachomatis infections in men and women by testing first-void urine by ligase chain reaction.
        J Clin Microbiol. 1994; 32: 2682
        • Chin D.P.
        • Yajko D.M.
        • Hadley W.K.
        • et al.
        Clinical utility of a commercial test based on the polymerase chain reaction for detecting Mycobacterium tuberculosis in respiratory specimens.
        Am J Resp Crit Care Med. 1995; 151: 1872
        • Dale B.
        • Dragon E.A.
        Polymerase chain reaction in infectious disease diagnosis.
        Lab Med. 1994; 25: 637

      11. Enns RK and the NCCLS Subcommittee on Molecular Microbiology: Molecular Diagnostic Methods for Infectious Diseases: Proposed guideline. NCCLS Medical Microbiology—Proposed Standard, 3-P, vol 14, no 4, 1994

        • Gaydos C.A.
        • Eiden J.J.
        • Oldach D.
        • et al.
        Diagnosis of Chlamydia pneumoniae infection in patients with community-acquired pneumonia by polymerase chain reaction enzyme immunoassay.
        Clin Infect Dis. 1994; 19: 157
        • Goessens W.H.F.
        • Kluytmans J.
        • den Toom N.
        • et al.
        Influence of volume of sample processed on detection of Chlamydia trachomatis in urogenital samples by PCR.
        J Clin Microbiol. 1995; 33: 251
        • Gufford T.
        • Dewilde A.
        • Lobert P.E.
        • et al.
        Significance and clinical relevance of the detection of herpes simplex virus DNA by the polymerase chain reaction in cerebrospinal fluid from patients with presumed encephalitis.
        Clin Infect Dis. 1994; 18: 744
        • Hook E.W.
        • Spitters C.
        • Reichart C.A.
        • et al.
        Use of cell culture and a rapid diagnostic assay for Chlamydia trachomatis screening.
        JAMA. 1994; 272: 867
        • Jackson J.B.
        • Drew J.
        • Lin J.J.
        • et al.
        Establishment of a quality assurance program for human immunodeficiency virus type 1 DNA polymerase chain reaction assays by the AIDS clinical trials group.
        J Clin Microbiol. 1993; 31: 3123
        • Johannisson G.
        • Lowhagen G-B
        • Lycke E.
        Genital Chlamydia trachomatis among sexually active asymptomatic young males.
        JAMA. 1993; 270: 2065
        • Kennedy N.
        • Gillespie S.H.
        • Sarvni A.O.S.
        • et al.
        Polymerase chain reaction for assessing treatment response in patients with pulmonary tuberculosis.
        J Infect Dis. 1994; 170: 713
        • Kessler H.H.
        • Reinthaler F.F.
        • Pschaid A.
        • et al.
        Rapid detection of Legionella species in bronchoalveolar lavage fluids with the EnviroAmp Legionella PCR amplification and detection kit.
        J Clin Microbiol. 1993; 31: 3325
        • Lee H.H.
        • Chemesky M.A.
        • Schachter J.
        • et al.
        Diagnosis of Chlamydia trachomatis genitourinary infection in women by ligase chain reaction assay of urine.
        Lancet. 1995; 245: 213
        • Loeffelholz M.J.
        • Lewinski C.A.
        • Silver S.R.
        • et al.
        Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction.
        J Clin Microbiol. 1992; 30: 2847
        • Mahony J.B.
        • Luinstra K.E.
        • Sellors J.W.
        • et al.
        Confirmatory polymerase chain reaction testing for Chlamydia trachomatis in first-void urine from asymptomatic and symptomatic men.
        J Clin Microbiol. 1992; 30: 2241
        • Mahony J.B.
        • Luinstra K.E.
        • Waner J.
        • et al.
        Inter-laboratory agreement study of a double set of PCR plasmid primers for detection of Chlamydia trachomatis in a variety of genitourinary specimens.
        J Clin Microbiol. 1994; 32: 87
        • Nims L.
        • Hatch K.
        • Gallaher M.
        • et al.
        Hemorrhage and shock associated with invasive pneumococcal infection in healthy infants and children—New Mexico 1993-1994.
        MMWR. 1995; 51-52: 949
        • Noordhoek G.T.
        • Kolk A.H.J.
        • Bjune G.
        • et al.
        Sensitivity and specificity of PCR for detection of Mycobacterium tuberculosis: A blind comparison study among seven laboratories.
        J Clin Microbiol. 1994; 32: 277
        • Panke E.S.
        • Yang L.I.
        • Leist P.A.
        • et al.
        Comparison of Gen-Probe DNA probe test and culture for the detection of Neisseria gonorrhoeae in endocervical specimens.
        J Clin Microbiol. 1991; 29: 883
        • Persing D.H.
        In vitro nucleic acid amplification techniques.
        in: Persing DH Smith TF Tenover FC Diagnostic Molecular Microbiology. American Society of Microbiologists, Washington, DC1993: 51
        • Plikaytis B.B.
        • Marden J.L.
        • Crawford J.T.
        • et al.
        Multiplex PCR assay specific for the multidrug-resistant strain W of Mycobacterium tuberculosis.
        J Clin Microbiol. 1994; 32: 1542
        • Roux P.
        • Lavrard I.
        • Poirot J.L.
        • et al.
        Usefulness of PCR for detection of Pneumocystis carinii DNA.
        J Clin Microbiol. 1994; 32: 2324
        • Rys P.N.
        • Persing D.H.
        Preventing false-positive: Quantitative evaluation of three protocols for inactivation of polymerase chain reaction amplification products.
        J Clin Microbiol. 1993; 31: 2356
        • Shafer M-A
        • Schachter J.
        • Moncada J.
        • et al.
        Evaluation of urine-based screening strategies to detect Chlamydia trachomatis among sexually active asymptomatic young males.
        JAMA. 1993; 270: 2065
        • Storch G.A.
        • Buller R.S.
        • Bailey T.C.
        • et al.
        Comparison of PCR and pp 65 antigenemia assay with quantitative shell vial culture for detection of cytomegalovirus in blood leukocytes from solid-organ transplant recipients.
        J Clin Microbiol. 1994; 32: 997
        • Tjhie J.H.T.
        • van Juppeveld F.J.M.
        • Roosendaal R.
        • et al.
        Direct PCR enables detection of Mycoplasma pneumoniae in patients with respiratory tract infections.
        J Clin Microbiol. 1994; 32: 11
        • Wadowsky R.M.
        • Laus S.
        • Libert T.
        • et al.
        Inhibition of PCR-based assay for Bordetella pertussis by using calcium alginate fiber and aluminum shaft components of a nasopharyngeal swab.
        J Clin Microbiol. 1994; 32: 1054
        • Wilson K.H.
        Detection of culture-resistant bacterial pathogens by amplification and sequencing of ribosomal DNA.
        Clin Infect Dis. 1994; 18: 958
        • Wolcott M.J.
        Advances in nucleic acid-based detection methods.
        Clin Microbiol Rev. 1992; 5: 370
        • Woods G.L.
        • Witebsky F.G.
        Current status of mycobacterial testing in clinical laboratories.
        Arch Pathol Lab Med. 1993; 117: 876

      Article info

      Identification

      DOI: https://doi.org/10.1016/S0272-2712(18)30286-5

      Copyright

      © 1996 Elsevier Inc. Published by Elsevier Inc. All rights reserved.

      ScienceDirect

      Access this article on ScienceDirect

      The content on this site is intended for healthcare professionals.


      We use cookies to help provide and enhance our service and tailor content. To update your cookie settings, please visit the Cookie Preference Center for this site.
      Copyright © 2023 Elsevier Inc. except certain content provided by third parties.


      RELX