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Research Article| Volume 16, ISSUE 1, P1-21, March 1996

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Clinical Use of B- and T-Cell Gene Rearrangement Analysis in Hematopoietic Disorders

  • John R. Krause
    Correspondence
    Address reprint requests to: John R. Krause, MD, Department of Clinical Pathology, Tulane Medical Center, 1415 Tulane Avenue, New Orleans, LA 70112
    Affiliations
    From the Department of Clinical Pathology, Tulane Medical Center, New Orleans, Louisiana
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      This paper is only available as a PDF. To read, Please Download here.
      The clinical application of B- and T-cell gene rearrangement analysis is discussed relative to diagnostic problems in specific lymphoprolifer ative disorders. This article reviews the use of the Southern blot hybridization technique for B- and T-cell gene rearrangement detection in the analysis of various hematopoietic lesions. Several case studies are presented and analyzed.
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      References

        • Ahmed T.
        • Wormser G.P.
        • Stahl R.E.
        • et al.
        Malignant lymphomas in a population at risk for acquired immune deficiency syndrome.
        Cancer. 1987; 60: 719-723
        • Azab M.B.
        • Henry-Amar M.
        • Rougier P.
        • et al.
        Prognostic factors in primary gastrointestinal non-Hodgkin’s lymphoma: A multivariate analysis, report of 107 cases and review of the literature.
        Cancer. 1989; 64: 1208-1217
        • Bernstein L.
        • Lewis D.
        • Mench I.T.
        • et al.
        AIDS related secular trends in cancer in Los Angeles county men: a comparison by marital status.
        Cancer Res. 1989; 49: 466-470
        • Chan J.K.C.
        • Ng C.S.
        • Isaacson P.G.
        Relationship between high grade lymphoma and low grade B cell mucosa associated lymphoid tissue lymphoma (Maltoma) of the stomach.
        Am J Pathol. 1990; 136: 1153-1164
        • Chan W.C.
        • Link S.
        • Mawle A.
        • et al.
        Heterogeneity of large granular lymphocyte proliferations: delineation of two major subtypes.
        Blood. 1986; 68: 1142-1153
        • Cohen D.J.
        • Loertsher R.
        • Rubin M.F.
        • et al.
        Cyclosporine: A new immunosuppressive agent for organ transplantation.
        Ann Intern Med. 1984; 101: 667-682
        • Cossman J.
        • Uppenkamp M.
        • Sundeen J.
        • et al.
        Molecular genetics and the diagnosis of lymphoma.
        Arch Pathol Lab Med. 1988; 112: 117-127
        • Dawson I.M.P.
        • Comes J.S.
        • Morson B.C.
        Primary malignant tumour of the intestinal tract.
        Br J Surg. 1961; 49: 80-89
        • Dosaka N.
        • Tanaka T.
        • Fujita M.
        • et al.
        Southern blotting analysis of clonal rearrangements of T-cell receptor genes in plaque lesions of mycosis fungoides.
        J Invest Dermatol. 1989; 93: 626-629
        • Evans H.L.
        Extranodal small lymphocytic proliferation: A clinicopathologic and immu-nocytochemical study.
        Cancer. 1982; 49: 84-96
        • Hanto D.W.
        • Gajl-Peczalska K.J.
        • Frizzara G.
        Epstein-Barr virus (EBV) induced polyclonal and monoclonal B-cell lymphoproliferative diseases occurring after renal transplantation. Clinical, pathologic, and Virologie findings and implications for therapy.
        Ann Surg. 1983; 198: 356-359
        • Harris N.L.
        • Harmon D.C.
        • Pileh B.Z.
        • et al.
        Immunohistologic diagnosis of orbital lymphoid infiltrates.
        Am J Surg Pathol. 1984; 8: 83-91
        • Hayes J.
        • Dunn E.
        Has the incidence of primary gastric lymphoma increased?.
        Cancer. 1989; 63: 2073-2076
        • Hoppe R.T.
        • Wood G.S.
        • Abel E.A.
        Mycosis fungoides.
        Curr Prob Cancer. 1990; 14: 295-361
        • Howard M.R.
        • McVerry B.A.
        T cell lymphoma in a haemophiliac positive for antibody to HIV.
        Br J Haematol. 1987; 67: 115
        • Knowles D.M.
        The extranodal lymphoid infiltrate: a diagnostic dilemma.
        Semin Diagn Pathol. 1985; 2: 147-151
        • Knowles D.M.
        • Jakobiec F.A.
        Malignant lymphomas and lymphoid hyperplasia that occurs in the ocular adnexa (orbit, conjunctivae, and eyelids).
        in: Knowles DM Neoplastic Hematopathology. Williams and Wilkins, Baltimore1992
        • Knowles D.M.
        • Jakobiec F.A.
        Orbital lymphoid neoplasms: A clinicopathologic study of 60 patients.
        Cancer. 1980; 46: 576-589
        • Knowles D.M.
        • Chamulak G.A.
        • Subar M.
        • et al.
        Lymphoid neoplasia associated with the acquired immunodeficiency syndrome (AIDS). The New York University Medical Center Experience with 105 patients (1981-86).
        Ann Intern Med. 1988; 108: 744-753
        • Knowles D.M.
        • Chamulak G.A.
        • Subar M.
        • et al.
        Clinicopathologic, immunophenotypic, and molecular genetic analysis of AIDS associated lymphoid neoplasia: clinical and biologic implications.
        Pathol Annu. 1988; 23: 33-67
        • Knowles D.M.
        • Nevi A.
        • Pelicci P.G.
        • et al.
        Immunoglobulin and T cell receptor beta chain gene rearrangement analysis of Hodgkin’s disease: Implications for disease determination and differential diagnosis.
        Proc Natl Acad Sci USA. 1986; 83: 7942-7946
        • Levine A.M.
        Non-Hodgkin’s lymphomas and other malignancies in the acquired immunodeficiency syndrome.
        Semin Oncol. 1987; 14: 34-39
        • Linden M.D.
        • Fishleder A.J.
        • Katzin W.E.
        • et al.
        Absence of B cell or T cell clinical expression in nodular, lymphocyte predominant Hodgkin’s disease.
        Hum Pathol. 1988; 19: 591-594
        • Lipford E.H.
        • Margolick J.B.
        • Longo D.L.
        • et al.
        Angiocentric immunoproliferative lesions: A clinicopathologic spectrum of post-thymic T-cell proliferations.
        Blood. 1988; 72: 1674-1681
        • Loughran T.P.
        Clonal disease of large granular lymphocytes.
        J Am Soc Hematol. 1993; 82: 1-14
        • Loughran T.P.
        Stark-cell receptor genes in large granular lymphocytic leukemia.
        Blood. 1988; 71: 822-824
        • Loughran T.P.
        • Starkebaum G.
        Large granular lymphocyte leukemia. Report of 38 cases and review of the literature.
        Medicine. 1987; 66: 397-405
        • Loughran T.P.
        • Clark E.A.
        • Price T.H.
        • et al.
        Adult onset cyclic neutropenia is associated with increased large granular lymphocytes.
        Blood. 1986; 68: 1082-1087
        • McKenna R.W.
        • Parkin J.
        • Kersey J.F.I.
        • et al.
        Chronic lymphoproliferative disorders with unusual clinical, morphologic, ultrastructural, and membrane surface marker characteristics.
        Am J Med. 1977; 62: 588-596
        • McNally L.
        • Jakobiec F.A.
        • Knowles D.M.
        Clinical, morphologic, immunophenotypic, and molecular genetic analysis of bilateral ocular adnexal lymphoid neoplasms in 17 patients.
        Am J Ophthal. 1987; 103: 5-68
        • Morgan G.
        • Harry J.
        Lymphocytic tumors of interterminate nature: A 5 year follow-up of 98 conjunctival and orbital lesions.
        Br J Ophthalmol. 1978; 62: 381-383
        • Nalesnik M.A.
        • Jaffe R.
        • Starzl T.E.
        • et al.
        The pathology of post transplant lymphoproliferative disorders occurring in the setting of cyclosporin A-prednisone immunosuppression.
        Am J Pathol. 1988; 133: 173-192
        • Nasu K.
        • Said J.
        • Vonderheid E.
        • et al.
        Immunopathology of cutaneous T-cell lymphomas.
        Am J Pathol. 1985; 119: 436-447
        • Neri A.
        • Jakobiec F.A.
        • Pelecci P.G.
        • et al.
        Immunoglobulin and T cell ß chain rearrangement analysis of ocular adnexal lymphoid neoplasms: Clinical and biologic implications.
        Blood. 1987; 70: 1519-1529
        • Pandolfi F.
        • Loughran T.P.
        • Starkebaum G.
        • et al.
        Clinical course and prognosis of the lymphoproliferative disease of granular lymphocytes. A multicenter study.
        Cancer. 1990; 65: 341
        • Presant C.A.
        • Gala K.
        • Wiseman C.
        • et al.
        Human immunodeficiency virus-associated T-cell lymphoblastic lymphoma in AIDS.
        Cancer. 1987; 60: 1459-1461
        • Sachs E.
        • Wieczovek R.
        • Jakobiec F.A.
        • et al.
        Lymphocyte subpopulations in the normal conjunctiva: a monoclonal antibody study.
        Ophthalmology. 1986; 93: 1276-1283
        • Southern E.M.
        Detection of specific sequences among DNA fragments separated by gel electrophoresis by gel electrophoresis.
        J Mol Biol. 1975; 98: 503-517
        • Spencer J.
        • Diss T.C.
        • Isaacson P.G.
        Primary B cell gastric lymphoma and “pseudo-lymphoma”: A genotypic analysis.
        Am J Pathol. 1989; 135: 57-564
        • Steis R.G.
        • Longo D.L.
        Clinic, biologic, and therapeutic aspects of malignancies associated with the acquired immunodeficiency syndrome.
        Part I: Ann Allergy. 1988; 60: 310-314
        • Weiss L.M.
        • Mouahed L.A.
        In situ demonstration of Epstein-Barr viral genomes in viral-associated B cell lymphoproliferations.
        Am J Pathol. 1989; 134: 651-659
        • Wood G.S.
        Recent advances in the molecular biology of cutaneous lymphomas and related disorders.
        Dermatology. 1991; 10: 172-177
        • Wright D.H.
        • Isaacson P.G.
        Gut associated lymphoid tumors.
        in: Whitehead R Gastrointestinal and Esophageal Pathology. Churchill-Livingstone, Edinburgh1989: 643-661