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Research Article| Volume 16, ISSUE 3, P617-639, September 1996

Polymerase Chain Reaction and other Amplification Techniques in Mycobacteriology

  • Ramon L. Sandin
    Correspondence
    Address reprint requests to: Ramon L. Sandin, MD, MS, Department of Pathology, Room 2071, H. Lee Moffitt Cancer, Center 12902 Magnolia Drive, University of South Florida, Tampa, FL 33612-9497
    Affiliations
    From the Clinical Microbiology, Clinical Virology, and Molecular Diagnostic Pathology Laboratories at H. Lee Moffitt Cancer Center and Research Institute, Department of Pathology, University of South Florida, Tampa, Florida
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DOI:https://doi.org/10.1016/S0272-2712(18)30258-0
Polymerase Chain Reaction and other Amplification Techniques in Mycobacteriology
Previous ArticleMethods for the Rapid Identification of Mycobacterial Species
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      Amplification methods for detection of Mycobacterium tuberculosis, such as polymerase chain reaction (PCR), have undergone much research and development in the last several years. The most common methods for extraction, amplification, and detection of mycobacterial nucleic acid sequences used in “in-house” PCR assays are discussed. A list of commercially prepared PCR and non-PCR amplification assays that should be available soon is included. The pros and cons of “in-house” versus commercial technology and issues of implementation of molecular technology in the clinical laboratory are reviewed.
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      DOI: https://doi.org/10.1016/S0272-2712(18)30258-0

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      © 1996 Elsevier Inc. Published by Elsevier Inc. All rights reserved.

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