Advertisement
Research Article| Volume 16, ISSUE 3, P551-567, September 1996

Primary Processing of Specimens and Isolation and Cultivation of Mycobacteria

  • Gerri S. Hall
    Correspondence
    Address reprint requests to: Gerri S. Hall, PhD, The Cleveland Clinic Foundation, 9500 Euclid Avenue (L-40), Cleveland, OH 44195–5140
    Affiliations
    From the Department of Clinical Pathology, The Cleveland Clinic Foundation, Cleveland, Ohio
    Search for articles by this author
DOI:https://doi.org/10.1016/S0272-2712(18)30255-5
Primary Processing of Specimens and Isolation and Cultivation of Mycobacteria
Previous ArticleBiosafety in the Clinical Mycobacteriology Laboratory
Next ArticleIdentification of Mycobacteria by Conventional Methods
      This paper is only available as a PDF. To read, Please Download here.
      Processing of specimens for mycobacteria need to proceed as rapidly as possible to provide 24-hour turnaround for smears and 21-day turnaround for the identification of Mycobacterium tuberculosis. If these goals are to be accomplished, a broth culture or microcolony plate method must be part of the media and in the planting process. Use of conventional solid media alone does not provide adequate turnaround time.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribers receive full online access to your subscription and archive of back issues up to and including 2002.

      Content published before 2002 is available via pay-per-view purchase only.

      Subscribe:

      Subscribe to Clinics in Laboratory Medicine
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Register: Create an account
      Institutional Access: Sign in to ScienceDirect

      References

        • Anargyros P.
        • Astill S.J.
        • Sim I.S.
        Comparison of improved BACTEC and Lowenstein-Jensen media for culture of mycobacteria from clinical specimens.
        J Clin Microbiol. 1990; 38: 1288-1291
        • Askgaard D.
        • Fuursted K.
        • Gottschau A.
        • et al.
        Detection of mycobacteria from blood and bone marrow: A decade of experience.
        APMIS. 1992; 100: 609-614
        • Babakhani F.K.
        • Warren N.G.
        • Henderson D.P.
        • et al.
        Effect of transportation and acid neutralization on recovery of mycobacteria from processed specimens.
        Am J Clin Pathol. 1995; 104: 65-68
        • Burdash N.M.
        • Manos J.P.
        • Ross D.
        • et al.
        Evaluation of the acid-fast smear.
        J Clin Microbiol. 1976; 4: 190-191
        • Conville P.S.
        • Andrews J.W.B.
        • Witebsky F.G.
        Effect of PANTA on growth of Mycobacterium kansasii in BACTEC 12B media.
        J Clin Microbiol. 1976; 33: 2012-2015
        • Eng R.H.K.
        • Bishburg E.
        • Smith S.M.
        • et al.
        Diagnosis of mycobacterium bacteremia in patients with acquired immunodeficiency syndrome by direct examination of blood films.
        J Clin Microbiol. 1989; 27: 768-769

      7. Evans K, Forthai D, Peterson EM: Detection of acid fast bacteria from blood cultures [abstract U-37]. In Abstracts of the 92nd General Meeting of American Society for Microbiology. New Orleans, 1992, p 171

        • Giger T.
        • Burkardt H.J.
        Evaluation of a new biphasic culture system for the recovery of mycobacteria.
        Eur J Clin Microbiol Infect Dis. 1990; 9: 428-431
        • Heifets L.B.
        Quantitative cultures and drug susceptibility testing of M. avium clinical isolates before and during the antimicrobial therapy. 11th forum in microbiology.
        Res Microbiology. 1994; 145: 188-196
        • Hall G.S.
        • Howard B.J.
        Mycobacteria.
        in: Howard BJ Keiser JF Smith TF Clinical and Pathogenic Microbiology. ed 2. Mosby-Year Book, St. Louis1994: 503-528
        • Hanna B.A.
        • Walters S.B.
        • Bonk S.J.
        • et al.
        Recovery of mycobacteria from blood in Mycobacteria Growth Indicator Tube (MGIT) and on Lowenstein-Jensen slant after lysis centrifucation.
        J Clin Microbiol. 1995; 33: 3315-3316
        • Hoffner S.E.
        • Haile M.
        • Kallenius G.
        A biphasic system for primary isolation of mycobacteria compared to solid medium and broth culture.
        J Med Microbiol. 1992; 37: 332-334
        • Isenberg H.D.
        • D’Amato R.F.
        • Heifets L.
        • et al.
        Collaborative feasibility study of a biphasic system (Roche SeptiChek AFB) for rapid detection and isolation of mycobacteria.
        J Clin Microbiol. 1991; 29: 1719-1722
        • Kiehn T.E.
        • Cammarata R.
        Comparative recoveries of M. avium-M. intracellulare from isolator lysis-centrifugation and BACTEC 13A blood culture systems.
        J Clin Microbiol. 1988; 26: 760-761
        • Kirihara J.M.
        • Hillier S.L.
        • Coyle M.S.
        Improved detection times for Mycobacterium avium and Mycobacterium tuberculosis with the BACTEC radiometric system.
        J Clin Microbiol. 1985; 22: 841-845
        • Kononov Y.
        • Dang-Ta K.
        • Heifets L.
        Effect of egg yolk on growth of Mycobacterium tuberculosis in 7H-12 liquid medium.
        J Clin Microbiol. 1988; 26: 1395-1397
        • Kubica G.P.
        • Kaufmann A.J.
        • Dye W.E.
        Comments on the use of the new mucolytic agent, N-acetyl-L-cysteine, as a sputum digestant for the isolation of mycobacteria.
        American Review of Respiratory Disease. 1964; 87: 775-779
        • Lipsky B.A.
        • Gates J.A.
        • Tenover F.C.
        • et al.
        Factors affecting the clinical value of microscopy for acid fast bacilli.
        Rev Infect Dis. 1984; 6: 214-222

      19. Lue YA, Visot B, Brophy G: Rapid recovery of M. avium complex (MAC) from blood specimens with Mycobacteria Growth Indicator Tube (MGIT) [abstract U-149]. In Abstracts of 95th General Meeting of American Society for Microbiology, Washington, DC, 1995, p 143

        • Morgan M.A.
        • Horstmeier C.D.
        • DeYoung D.R.
        • et al.
        Comparison of a radiometric (BACTEC) and conventional culture media for recovery of mycobacteria from smear negative specimens.
        J Clin Microbiol. 1983; 18: 364-368
        • Murray P.R.
        • Elmore C.
        • Krogstad D.J.
        The acid-fast stain: A specific and predictive test for mycobacterial disease.
        Ann Intern Med. 1980; 92: 512-513

      22. Murtagh JJ, Muthukutty J, Kimani AP, et al: Detection and speciation of mycobacteria by immunomagnetic and bioluminescent techniques. In Abstracts of International Conference of The American Thoracic Society, 1995

        • Nolte F.S.
        • Metchock B.
        Mycobacteria.
        in: Murray PR Baron EJ Pfaller MA Manual of Clinical Microbiology. ed 6. ASM Press, Washington, DC1995: 400-437
        • Park C.H.
        • Hixon D.L.
        • Ferguson C.B.
        • et al.
        Rapid recovery of mycobacteria from clinical specimens using automated radiometric technique.
        Am J Clin Pathol. 1984; 81: 341-345
        • Piersimoni C.
        • Morbiducci V.
        • DeSio G.
        • et al.
        Comparative evaluation of the MB-check system for recovery of mycobacteria from clinical specimens.
        Eur J Clin Microbiol Infect Dis. 1992; 11: 1174-1177
        • Pollock H.M.
        • Wieman E.J.
        Smear results in the diagnosis of mycobacterioses using blue light fluorescence microscopy.
        J Clin Microbiol. 1977; 5: 329-331
        • Rickman T.W.
        • Moyer N.P.
        Increased sensitivity of acid-fast smears.
        J Clin Microbiol. 1980; 11: 618-620
        • Roberts G.D.
        • Goodman N.L.
        • Heifets L.
        • et al.
        Evaluation of the BACTEC radiometric method for recovery of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis from acid-fast smear positive specimens.
        J Clin Microbiol. 1983; 18: 689-696
        • Runyon E.H.
        Identification of mycobacterial pathogens utilizing colony characteristics..
        Am J Clin Pathol. 1970; 54: 578-586
        • Sewell D.L.
        • Rashad A.L.
        • Rourke W.J.
        • et al.
        Comparison of the Septi-Chek AFB and BACTEC systems and conventional culture for recovery of mycobacteria.
        J Clin Microbiol. 1993; 31: 2689-2691
        • Shinnick T.M.
        • Good R.C.
        Diagnostic mycobacteriology laboratory practices.
        Clin Infect Dis. 1995; 21: 291-299
        • Smithwick R.W.
        • Bigbie M.R.
        • Ferguson R.B.
        • et al.
        Phenolic acridine orange fluorescent stain for mycobacteria.
        J Clin Microbiol. 1995; 33: 2763-2764
        • Stager C.E.
        • Libonati J.P.
        • Siddiqi S.H.
        • et al.
        Role of solid media when used in conjunction with the BACTEC system for mycobacterial isolation and identification.
        J Clin Microbiol. 1991; 29: 154-157

      34. Stitt DT, Kodsi SE: A rapid method for the growth and detection of mycobacteria in clinical and stock cultures [abstract C-114]. In Abstracts of the 94th General Meeting American Society for Clinical Microbiology, Las Vegas, 1994, p 510

        • Stone B.L.
        • Cohn D.L.
        • Kane M.S.
        • et al.
        Utility of paired blood cultures and smears in diagnosis of disseminated Mycobacterium avium complex infections in AIDS patients.
        J Clin Microbiol. 1994; 32: 841-842
        • Tenover F.C.
        • Crawford J.T.
        • Heubner R.E.
        • et al.
        The resurgence of tuberculosis: Is your laboratory ready?.
        J Clin Microbiol. 1993; 31: 767-770
        • Tortoli E.
        • Mandler F.
        • Bartolucci M.
        • et al.
        Multicenter evaluation of a biphasic culture system for recovery of mycobacteria from clinical specimens.
        Eur J Clin Microbiol Infect Dis. 1993; 12: 425-429
        • Trifiro S.
        • Bourgault A.M.
        • Lebel F.
        • et al.
        Ghost mycobacteria on Gram stain.
        J Clin Microbiol. 1990; 28: 146-147
        • Truffot-Pernot C.
        • Lecoeur H.F.
        • Maury L.
        • et al.
        Results of blood cultures for detection of mycobacteria in AIDS patients.
        Tubercle. 1989; 70: 187-191
        • Wasilauskas B.
        • Morrell R.
        Inhibitory effect of isolator blood culture system on growth of Mycobacterium avium-M. intracellulare in BACTEC 12B bottles.
        J Clin Microbiol. 1994; 32: 654-657
        • Welch D.F.
        • Guruswamy A.P.
        • Sides S.J.
        • et al.
        Timely culture for mycobacteria which utilizes a microcolony method.
        J Clin Microbiol. 1993; 31: 2178-2184
        • Whittier S.
        • Hopfer R.L.
        • Knowles M.R.
        • et al.
        Improved recovery of mycobacteria from respiratory specimens of patients with cystic fibrosis.
        J Clin Microbiol. 1993; 31: 861-864
        • Wilson M.L.
        • Stone B.L.
        • Hildred M.V.
        • et al.
        Comparison of recovery rates for mycobacteria from BACTEC 12B vials, Middlebrook 7H11 selective biplates and Lowenstein-Jensen slants in a public health mycobacteriology laboratory.
        J Clin Microbiol. 1995; 33: 2516-2518
        • Witebsky F.G.
        • Keiser J.F.
        • Conville P.S.
        • et al.
        Comparison of BACTEC 13A medium and DuPont Isolator for detection of mycobacteremia.
        J Clin Microbiol. 1988; 26: 1501-1505
        • Woods G.L.
        • Pentony E.
        • Boxley M.J.
        • et al.
        Concentration of sputum by cytocentrifugation for preparation of smears for detection of acid-fast bacilli does not increase sensitivity of the fluorochrome stain.
        J Clin Microbiol. 1995; 33: 1915-1916
        • Woods G.L.
        • Witebsky F.G.
        Mycobacterial testing in clinical laboratories that participate in the College of American Pathologists Mycobacteriology E Survey: Results of a 1993 questionnaire.
        J Clin Microbiol. 1995; 33: 407-412
        • Yajko D.M.
        • Nassos P.S.
        • Sanders C.A.
        • et al.
        Predictive value of the acid fast smear for tuberculosis in a setting where M. avium complex is a common isolate.
        Clin Infect Dis. 1994; 19: 334-336

      Article info

      Identification

      DOI: https://doi.org/10.1016/S0272-2712(18)30255-5

      Copyright

      © 1996 Elsevier Inc. Published by Elsevier Inc. All rights reserved.

      ScienceDirect

      Access this article on ScienceDirect
      We use cookies to help provide and enhance our service and tailor content. To update your cookie settings, please visit the for this site.
      Copyright © 2023 Elsevier Inc. except certain content provided by third parties. The content on this site is intended for healthcare professionals.


      RELX