Research Article| Volume 17, ISSUE 2, P299-314, June 1997

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Specialized Imaging Techniques

  • Livingston Van De Water
    Address reprint requests to Livingston Van De Water, Shriners Bums Institute, Building 1400 West, One Kendall Square, Cambridge, MA 02139
    From the Departments of Surgery and the Center for Engineering in Medicine, Harvard Medical School, Massachusetts General Hospital, and the Shriners Bums Institute, Boston, Massachusetts
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      This paper is only available as a PDF. To read, Please Download here.
      This article reviews the theory and application of key optical microscopy techniques used in basic research and clinical medicine. Optical sectioning methodologies, such as confocal microscopy and image deconvolution, are discussed, as are fluorescent microscopy and the use of organelle-specific probes, fluorescent in situ hybridization techniques and ion specific probes. The article begins with a review of brightfield, darkfield, phase contrast, interference reflection, polarization and differential interference contrast microscopy.
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