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Research Article| Volume 20, ISSUE 2, P289-301, June 2000

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In Situ Diagnosis of Human Papillomaviruses

  • Elizabeth R. Unger
    Correspondence
    Address reprint requests to: Elizabeth R. Unger, PhD, MD, Centers for Disease Control and Prevention, 1600 Clifton Road, MSG18, Atlanta, GA 30333
    Affiliations
    From the Human Papillomavirus Section, Division of Viral and Rickettsial Diseases, Viral Exanthems and Herpesvirus Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia
    Search for articles by this author
DOI:https://doi.org/10.1016/S0272-2712(18)30063-5
In Situ Diagnosis of Human Papillomaviruses
Previous ArticleDiagnosis of Human Papillomavirus Gynecologic Infections
Next ArticleEvaluation of Abnormal Cervical Cytology
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      In situ hybridization (ISH) is the demonstration of specific genetic information within a morphologic context. For HPV, colorimetric ISH has the advantage that it can be applied to routine formalin-fixed paraffin embedded tissues. This conserves patient material and permits histologic selection of optimal material for testing. ISH allows for precise spatial localization of viral sequences within tissues. ISH also allows the integration status of HPV to be determined. The major limitations of the method are the potential for error in HPV typing because of probe cross-hybridization and relatively low sensitivity if the method is not fully optimized.
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      DOI: https://doi.org/10.1016/S0272-2712(18)30063-5

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      © 2000 Elsevier Inc. Published by Elsevier Inc. All rights reserved.

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