Research Article| Volume 20, ISSUE 2, P289-301, June 2000

Download started.


In Situ Diagnosis of Human Papillomaviruses

  • Elizabeth R. Unger
    Address reprint requests to: Elizabeth R. Unger, PhD, MD, Centers for Disease Control and Prevention, 1600 Clifton Road, MSG18, Atlanta, GA 30333
    From the Human Papillomavirus Section, Division of Viral and Rickettsial Diseases, Viral Exanthems and Herpesvirus Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia
    Search for articles by this author
      This paper is only available as a PDF. To read, Please Download here.
      In situ hybridization (ISH) is the demonstration of specific genetic information within a morphologic context. For HPV, colorimetric ISH has the advantage that it can be applied to routine formalin-fixed paraffin embedded tissues. This conserves patient material and permits histologic selection of optimal material for testing. ISH allows for precise spatial localization of viral sequences within tissues. ISH also allows the integration status of HPV to be determined. The major limitations of the method are the potential for error in HPV typing because of probe cross-hybridization and relatively low sensitivity if the method is not fully optimized.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribers receive full online access to your subscription and archive of back issues up to and including 2002.

      Content published before 2002 is available via pay-per-view purchase only.


      Subscribe to Clinics in Laboratory Medicine
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Capodieci P.
        • Magi-Galluzzi C.
        • Moreira G.
        • et al.
        Automated in situ hybridization: Diagnostic and research applications.
        Diagn Mol Pathol. 1998; 7: 69
        • Chenggis M.L.
        • Unger E.R.
        Application of a manual capillary action workstation to colorimetric in situ hybridization.
        Journal of Histotechnology. 1993; 16: 33
        • Cheung A.L.M.
        • Graf A-H
        • Hauser-Kronberger
        • et al.
        Detection of human papillomavirus in cervical carcinoma: Comparison of peroxidase, nanogold, and catalyzed reported deposition (CARD)-nanogold in situ hybridization.
        Mod Pathol. 1999; 12: 689
        • Cox K.H.
        • DeLeon D.V.
        • Angerer L.M.
        • et al.
        Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes.
        Dev Biol. 1984; 101: 485
        • Kerstens H.M.J.
        • Poddighe P.J.
        • Hanselaar A.G.J.M.
        A novel in situ hybridization method based on the deposition of biotinylated tyramide.
        J Histochem Cytochem. 1995; 43: 347
        • Lan H.Y.
        • Mu W.
        • Ng Y.Y.
        • et al.
        A simple, reliable and sensitive method for nonradioactive in situ hybridization: Use of microwave heating to improve hybridization efficiency and preserve tissue morphology.
        J Histochem Cytochem. 1996; 44: 281
        • Plummer T.B.
        • Sperry A.C.
        • Xu H.S.
        • et al.
        In situ hybridization detection of low copy nucleic acid sequences using catalyzed reporter deposition and its usefulness in clinical human papillomavirus typing.
        Diagn Mol Pathol. 1998; 7: 76
        • Sano T.
        • Hikino T.
        • Niwa Y.
        • et al.
        In situ hybridization with biotinylated tyramide amplification: Detection of human papillomavirus DNA in cervical neoplastic lesions.
        Mod Pathol. 1998; 11: 19
        • Southern S.A.
        • Graham D.A.
        • Herrington C.S.
        Discrimination of human papillomavirus types in low and high grade cervical squamous neoplasia.
        Diagn Mol Pathol. 1998; 7: 114
        • Stoler M.H.
        In situ hybridization.
        Clin Lab Med. 1990; 10: 215
        • Unger E.R.
        • Brigati D.J.
        • Chenggis M.L.
        • et al.
        Automation of in situ hybridization: Application of the capillary action robotic workstation.
        Journal of Histotechnology. 1988; 11: 253
        • Unger E.R.
        • Hammer M.L.
        • Chenggis M.L.
        Comparison of 35S and biotin as labels for in situ hybridization: Use of an HPV model system.
        J Histochem Cytochem. 1991; 39: 145
        • Unger E.R.
        • Vernon S.D.
        • Hewan-Lowe K.O.
        • et al.
        An unusual cervical carcinoma showing exception to epitheliotropism of human papillomavirus.
        Hum Pathol. 1999; 30: 483
        • Unger E.R.
        • Vernon S.D.
        • Lee D.R.
        • et al.
        Detection of human papillomavirus in archival tissues: Comparison of in situ hybridization and polymerase chain reaction.
        J Histochem Cytochem. 1998; 46: 535
        • Unger E.R.
        • Vernon S.D.
        • Lee D.R.
        • et al.
        Human papillomavirus type in anal epithelial lesions is influenced by human immunodeficiency virus.
        Arch Pathol Lab Med. 1997; 121: 820
        • Unger E.R.
        • Vernon S.D.
        • Thoms W.W.
        • et al.
        Human papillomavirus and disease-free survival in FIGO stage lb cervical cancer.
        J Infect Dis. 1995; 172: 1184
        • Wilcox J.N.
        Fundamental principles of in situ hybridization.
        J Histochem Cytochem. 1993; 41: 1725