Review Article| Volume 37, ISSUE 4, P869-878, December 2017

Mast Cell Disease Assessment by Flow Cytometric Analysis

Published:October 10, 2017DOI:


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        • Akin C.
        Mast cell activation syndrome: proposed diagnostic criteria.
        J Allergy Clin Immunol. 2010; 126 (Available at:): 1104.e4
        • Metcalfe D.D.
        Mast cells and mastocytosis.
        Blood. 2008; 112: 946-956
        • Okayama Y.
        • Kawakami T.
        Development, migration, and survival of mast cells.
        Immunol Res. 2006; 34: 97-115
        • Arber D.A.
        • Orazi A.
        • Hasserjian R.
        • et al.
        The 2016 revision to the World Health Organization classification of myeloid neoplasms and acute leukemia.
        Blood. 2016; 127: 2391-2405
        • Swerdlow S.H.
        • Campo E.
        • Harris N.L.
        • et al.
        4th edition. WHO classification of tumours of haematopoietic and lymphoid tissues. vol. 2. Internat. Agency for Research on Cancer, Lyon (France)2008 (2. print)
        • Sánchez-Muñoz L.
        • Teodosio C.
        • Morgado J.M.T.
        • et al.
        Flow cytometry in mastocytosis: utility as a diagnostic and prognostic tool.
        Immunol Allergy Clin North Am. 2014; 34: 297-313
        • Morgado J.M.
        • Perbellini O.
        • Johnson R.C.
        • et al.
        CD30 expression by bone marrow mast cells from different diagnostic variants of systemic mastocytosis.
        Histopathology. 2013; 63 (Available at:) (Accessed August 29, 2017): 780-787
        • Álvarez-Twose I.
        • Jara-Acevedo M.
        • Morgado J.M.
        • et al.
        Clinical, immunophenotypic, and molecular characteristics of well-differentiated systemic mastocytosis.
        J Allergy Clin Immunol. 2016; 137: 178.e1
        • Akin C.
        • Escribano L.
        • Nuñez R.
        • et al.
        Well-differentiated systemic mastocytosis: a new disease variant with mature mast cell phenotype and lack of codon 816 c-kit mutations.
        J Allergy Clin Immunol. 2004; 113: S327
        • Akin C.
        • Scott L.M.
        • Kocabas C.N.
        • et al.
        Demonstration of an aberrant mast-cell population with clonal markers in a subset of patients with “idiopathic” anaphylaxis.
        Blood. 2007; 110: 2331-2333
        • Iwaki S.
        • Spicka J.
        • Tkaczyk C.
        • et al.
        Kit- and fc epsilonRI-induced differential phosphorylation of the transmembrane adaptor molecule NTAL/LAB/LAT2 allows flexibility in its scaffolding function in mast cells.
        Cell Signal. 2008; 20: 195-205
        • Matarraz S.
        • López A.
        • Barrena S.
        • et al.
        The immunophenotype of different immature, myeloid and B-cell lineage-committed CD34+ hematopoietic cells allows discrimination between normal/reactive and myelodysplastic syndrome precursors.
        Leukemia. 2008; 22: 1175-1183
        • Schernthaner G.H.
        • Hauswirth A.W.
        • Baghestanian M.
        • et al.
        Detection of differentiation- and activation-linked cell surface antigens on cultured mast cell progenitors.
        Allergy. 2005; 60: 1248-1255
        • Shimizu Y.
        • Suga T.
        • Maeno T.
        • et al.
        Detection of tryptase-, chymase+ cells in human CD34 bone marrow progenitors.
        Clin Exp Allergy. 2004; 34: 1719-1724
        • Tedla N.
        • Lee C.
        • Borges L.
        • et al.
        Differential expression of leukocyte immunoglobulin-like receptors on cord-blood-derived human mast cell progenitors and mature mast cells.
        J Leukoc Biol. 2008; 83: 334-343
        • Dahl C.
        • Hoffmann H.J.
        • Saito H.
        • et al.
        Human mast cells express receptors for IL-3, IL-5 and GM-CSF; a partial map of receptors on human mast cells cultured in vitro.
        Allergy. 2004; 59: 1087-1096
        • Yokoi H.
        • Myers A.
        • Matsumoto K.
        • et al.
        Alteration and acquisition of siglecs during in vitro maturation of CD34+ progenitors into human mast cells.
        Allergy. 2006; 61: 769-776
        • Teodosio C.
        • García-Montero A.C.
        • Jara-Acevedo M.
        • et al.
        Mast cells from different molecular and prognostic subtypes of systemic mastocytosis display distinct immunophenotypes.
        J Allergy Clin Immunol. 2010; 125: 726.e4
        • Orfao A.
        • Escribano L.
        • Villarrubia J.
        • et al.
        Flow cytometric analysis of mast cells from normal and pathological human bone marrow samples: identification and enumeration.
        Am J Pathol. 1996; 149: 1493-1499
        • Escribano L.
        • Diaz-Agustin B.
        • López A.
        • et al.
        Immunophenotypic analysis of mast cells in mastocytosis: when and how to do it. proposals of the Spanish network on mastocytosis (REMA).
        Cytometry B Clin Cytom. 2004; 58: 1-8
        • Escribano L.
        • Dı́az-Agustı́n B.
        • Bellas C.
        • et al.
        Utility of flow cytometric analysis of mast cells in the diagnosis and classification of adult mastocytosis.
        Leuk Res. 2001; 25: 563-570
        • Pozdnyakova O.
        • Kondtratiev S.
        • Li B.
        • et al.
        High-sensitivity flow cytometric analysis for the evaluation of systemic mastocytosis including the identification of a new flow cytometric criterion for bone marrow involvement.
        Am J Clin Pathol. 2012; 138: 416-424
        • Pardanani A.
        • Kimlinger T.
        • Reeder T.
        • et al.
        Bone marrow mast cell immunophenotyping in adults with mast cell disease: a prospective study of 33 patients.
        Leuk Res. 2004; 28: 777-783
        • Jabbar K.J.
        • Medeiros L.J.
        • Wang S.A.
        • et al.
        Flow cytometric immunophenotypic analysis of systemic mastocytosis involving bone marrow.
        Arch Pathol Lab Med. 2014; 138: 1210-1214
        • Morgado J.M.T.
        • Sanchez-Munoz L.
        • Teodosio C.G.
        • et al.
        Immunophenotyping in systemic mastocytosis diagnosis: ‘CD25 positive’ alone is more informative than the 'CD25 and/or CD2' WHO criterion.
        Mod Pathol. 2012; 25: 516-521
        • Cherian S.
        • McCullouch V.
        • Miller V.
        • et al.
        Expression of CD2 and CD25 on mast cell populations can be seen outside the setting of systemic mastocytosis.
        Cytometry B Clin Cytom. 2016; 90B: 387-392
        • Pozdnyakova O.
        • Laplante C.D.
        • Li B.
        • et al.
        High-sensitivity flow cytometric analysis of mast cell clustering in systemic mastocytosis: a quantitative and statistical analysis.
        Leuk Lymphoma. 2015; 56: 1735-1741
        • Dorfman D.M.
        • LaPlante C.D.
        • Pozdnyakova O.
        • et al.
        FLOCK cluster analysis of mast cell event clustering by high-sensitivity flow cytometry predicts systemic mastocytosis.
        Am J Clin Pathol. 2015; 144: 764-770
        • Chisholm K.M.
        • Merker J.D.
        • Gotlib J.R.
        • et al.
        Mast cells in systemic mastocytosis have distinctly brighter CD45 expression by flow cytometry.
        Am J Clin Pathol. 2015; 143: 527-534