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Review Article| Volume 35, ISSUE 2, P461-486, June 2015

Multiplex Polymerase Chain Reaction Tests for Detection of Pathogens Associated with Gastroenteritis

Published:April 03, 2015DOI:https://doi.org/10.1016/j.cll.2015.02.006

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      References

        • Farthing M.
        • Salam M.A.
        • Lindberg G.
        • et al.
        Acute diarrhea in adults and children: a global perspective.
        J Clin Gastroenterol. 2013; 47: 12-20
        • Kosek M.
        • Bern C.
        • Guerrant R.L.
        The global burden of diarrhoeal disease, as estimated from studies published between 1992 and 2000.
        Bull World Health Organ. 2003; 81: 197-204
        • Thielman N.M.
        • Guerrant R.L.
        Clinical practice. Acute infectious diarrhea.
        N Engl J Med. 2004; 350: 38-47
        • Bresee J.S.
        • Marcus R.
        • Venezia R.A.
        • et al.
        The etiology of severe acute gastroenteritis among adults visiting emergency departments in the United States.
        J Infect Dis. 2012; 205: 1374-1381
        • Guerrant R.L.
        • Kosek M.
        • Moore S.
        • et al.
        Magnitude and impact of diarrheal diseases.
        Arch Med Res. 2002; 33: 351-355
        • Liu L.
        • Johnson H.L.
        • Cousens S.
        • et al.
        Global, regional, and national causes of child mortality: an updated systematic analysis for 2010 with time trends since 2000.
        Lancet. 2012; 379: 2151-2161
        • Walker C.L.
        • Rudan I.
        • Liu L.
        • et al.
        Global burden of childhood pneumonia and diarrhoea.
        Lancet. 2013; 381: 1405-1416
        • Wikswo M.E.
        • Hall A.J.
        Outbreaks of acute gastroenteritis transmitted by person-to-person contact–United States, 2009-2010.
        MMWR Surveill Summ. 2012; 61: 1-12
        • Lucado J.
        • Mohamoud S.
        • Zhao L.
        • et al.
        Infectious enteritis and foodborne illness in the United States, 2010 statistical brief #150. Healthcare Cost and Utilization Project (HCUP) statistical briefs.
        ([Internet]) Agency for Health Care Policy and Research (US), Rockville (MD)2013 (Available at:) (Accessed November 1, 2014)
        • Hofreuter D.
        Defining the metabolic requirements for the growth and colonization capacity of Campylobacter jejuni.
        Front Cell Infect Microbiol. 2014; 4: 137
        • Chen H.M.
        • Wang Y.
        • Su L.H.
        • et al.
        Nontyphoid salmonella infection: microbiology, clinical features, and antimicrobial therapy.
        Pediatr Neonatol. 2013; 54: 147-152
        • Blanton L.H.
        • Adams S.M.
        • Beard R.S.
        • et al.
        Molecular and epidemiologic trends of caliciviruses associated with outbreaks of acute gastroenteritis in the United States, 2000-2004.
        J Infect Dis. 2006; 193: 413-421
        • de Wit M.A.
        • Koopmans M.P.
        • van Duynhoven Y.T.
        Risk factors for norovirus, Sapporo-like virus, and group A rotavirus gastroenteritis.
        Emerg Infect Dis. 2003; 9: 1563-1570
        • Bok K.
        • Green K.Y.
        Norovirus gastroenteritis in immunocompromised patients.
        N Engl J Med. 2012; 367: 2126-2132
        • Katz D.E.
        • Taylor D.N.
        Parasitic infections of the gastrointestinal tract.
        Gastroenterol Clin North Am. 2001; 30: 797-815
        • Mehta S.
        • Fantry L.
        Gastrointestinal infections in the immunocompromised host.
        Curr Opin Gastroenterol. 2005; 21: 39-43
        • Dusch H.
        • Altwegg M.
        Evaluation of five new plating media for isolation of Salmonella species.
        J Clin Microbiol. 1995; 33: 802-804
        • Perez J.M.
        • Cavalli P.
        • Roure C.
        • et al.
        Comparison of four chromogenic media and Hektoen agar for detection and presumptive identification of Salmonella strains in human stools.
        J Clin Microbiol. 2003; 41: 1130-1134
        • Perry J.D.
        • Ford M.
        • Taylor J.
        • et al.
        ABC medium, a new chromogenic agar for selective isolation of Salmonella spp.
        J Clin Microbiol. 1999; 37: 766-768
        • Bennett Jr., W.E.
        • Tarr P.I.
        Enteric infections and diagnostic testing.
        Curr Opin Gastroenterol. 2009; 25: 1-7
        • Gaillot O.
        • di Camillo P.
        • Berche P.
        • et al.
        Comparison of CHROMagar Salmonella medium and Hektoen enteric agar for isolation of salmonellae from stool samples.
        J Clin Microbiol. 1999; 37: 762-765
        • Guerrant R.L.
        • Van Gilder T.
        • Steiner T.S.
        • et al.
        Practice guidelines for the management of infectious diarrhea.
        Clin Infect Dis. 2001; 32: 331-351
        • Kebede A.
        • Verweij J.J.
        • Petros B.
        • et al.
        Short communication: Misleading microscopy in amoebiasis.
        Trop Med Int Health. 2004; 9: 651-652
        • Svensson A.M.
        • LaSala P.R.
        Pathology consultation on detection of Clostridium difficile.
        Am J Clin Pathol. 2012; 137: 10-15
        • Dunbar S.A.
        • Zhang H.
        • Tang Y.W.
        Advanced techniques for detection and identification of microbial agents of gastroenteritis.
        Clin Lab Med. 2013; 33: 527-552
        • Platts-Mills J.A.
        • Kosek M.
        Update on the burden of Campylobacter in developing countries.
        Curr Opin Infect Dis. 2014; 27: 444-450
        • Platts-Mills J.A.
        • Liu J.
        • Gratz J.
        • et al.
        Detection of Campylobacter in stool and determination of significance by culture, enzyme immunoassay, and PCR in developing countries.
        J Clin Microbiol. 2014; 52: 1074-1080
      1. Cepheid Xpert Norovirus assay. Available at: http://www.cepheid.com/administrator/components/com_productcatalog/library-files/f5e5a03863c93410f24f504b8aafa955-Xpert-Norovirus-Brochure-EU-3013-04.pdf. Accessed November 1, 2014.

        • Ahmed S.M.
        • Hall A.J.
        • Robinson A.E.
        • et al.
        Global prevalence of norovirus in cases of gastroenteritis: a systematic review and meta-analysis.
        Lancet Infect Dis. 2014; 14: 725-730
        • Rocha-Pereira J.
        • Neyts J.
        • Jochmans D.
        Norovirus: targets and tools in antiviral drug discovery.
        Biochem Pharmacol. 2014; 91: 1-11
        • Mahony J.B.
        • Blackhouse G.
        • Babwah J.
        • et al.
        Cost analysis of multiplex PCR testing for diagnosing respiratory virus infections.
        J Clin Microbiol. 2009; 47: 2812-2817
        • Wu W.
        • Tang Y.W.
        Emerging molecular assays for detection and characterization of respiratory viruses.
        Clin Lab Med. 2009; 29: 673-693
        • Yan Y.
        • Zhang S.
        • Tang Y.W.
        Molecular assays for the detection and characterization of respiratory viruses.
        Semin Respir Crit Care Med. 2011; 32: 512-526
        • Ginocchio C.C.
        • Zhang F.
        • Manji R.
        • et al.
        Evaluation of multiple test methods for the detection of the novel 2009 influenza A (H1N1) during the New York City outbreak.
        J Clin Virol. 2009; 45: 191-195
        • Pierce V.M.
        • Hodinka R.L.
        Comparison of the GenMark Diagnostics eSensor respiratory viral panel to real-time PCR for detection of respiratory viruses in children.
        J Clin Microbiol. 2012; 50: 3458-3465
        • Buller R.S.
        Molecular detection of respiratory viruses.
        Clin Lab Med. 2013; 33: 439-460
        • Liu J.
        • Kabir F.
        • Manneh J.
        • et al.
        Development and assessment of molecular diagnostic tests for 15 enteropathogens causing childhood diarrhoea: a multicentre study.
        Lancet Infect Dis. 2014; 14: 716-724
        • Houpt E.
        • Gratz J.
        • Kosek M.
        • et al.
        Microbiologic methods utilized in the MAL-ED cohort study.
        Clin Infect Dis. 2014; 59: S225-S232
        • Gray J.
        • Coupland L.J.
        The increasing application of multiplex nucleic acid detection tests to the diagnosis of syndromic infections.
        Epidemiol Infect. 2014; 142: 1-11
        • Wessels E.
        • Rusman L.G.
        • van Bussel M.J.
        • et al.
        Added value of multiplex Luminex Gastrointestinal Pathogen Panel (xTAG® GPP) testing in the diagnosis of infectious gastroenteritis.
        Clin Microbiol Infect. 2014; 20: O182-O187
        • Navidad J.F.
        • Griswold D.J.
        • Gradus M.S.
        Evaluation of Luminex xTAG gastrointestinal pathogen analyte-specific reagents for high-throughput, simultaneous detection of bacteria, viruses, and parasites of clinical and public health importance.
        J Clin Microbiol. 2013; 51: 3018-3024
        • Goldfarb D.M.
        • Dixon B.
        • Moldovan I.
        • et al.
        Nanolitre real-time PCR detection of bacterial, parasitic, and viral agents from patients with diarrhoea in Nunavut, Canada.
        Int J Circumpolar Health. 2013; 72: 19903
        • Khamrin P.
        • Okame M.
        • Thongprachum A.
        • et al.
        A single-tube multiplex PCR for rapid detection in feces of 10 viruses causing diarrhea.
        J Virol Methods. 2011; 173: 390-393
        • Pang X.L.
        • Preiksaitis J.K.
        • Lee B.E.
        Enhanced enteric virus detection in sporadic gastroenteritis using a multi-target real-time PCR panel: a one-year study.
        J Med Virol. 2014; 86: 1594-1601
        • Feeney S.A.
        • Armstrong V.J.
        • Mitchell S.J.
        • et al.
        Development and clinical validation of multiplex TaqMan® assays for rapid diagnosis of viral gastroenteritis.
        J Med Virol. 2011; 83: 1650-1656
        • Rundell M.S.
        • Pingle M.
        • Das S.
        • et al.
        A multiplex PCR/LDR assay for simultaneous detection and identification of the NIAID category B bacterial food and water-borne pathogens.
        Diagn Microbiol Infect Dis. 2014; 79: 135-140
        • Al-Talib H.
        • Latif B.
        • Mohd-Zain Z.
        Pentaplex PCR assay for detection of hemorrhagic bacteria from stool samples.
        J Clin Microbiol. 2014; 52: 3244-3249
        • Chankhamhaengdecha S.
        • Hadpanus P.
        • Aroonnual A.
        • et al.
        Evaluation of multiplex PCR with enhanced spore germination for detection of Clostridium difficile from stool samples of the hospitalized patients.
        Biomed Res Int. 2013; 2013: 875437
        • Heidary M.
        • Momtaz H.
        • Madani M.
        Characterization of diarrheagenic antimicrobial resistant Escherichia coli isolated from pediatric patients in Tehran, Iran.
        Iran Red Crescent Med J. 2014; 16: e12329
        • Wang X.G.
        • Zhang Y.H.
        • Wang P.
        • et al.
        Establishment and application of multiplex PCR for non-O157 H7 STEC virulence genes detection.
        Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2013; 27: 388-391
      2. Luminex xTAG GPP K121894. 2013. Available at: http://www.accessdata.fda.gov/cdrh_docs/reviews/K121894.pdf. Accessed November 1, 2014.

      3. Luminex xTAG GPP K140377. 2014. Available at: http://www.accessdata.fda.gov/cdrh_docs/reviews/k140377.pdf. Accessed November 1, 2014.

      4. BioFire’s FilmArray GI panel K140407. 2014. Available at: http://www.accessdata.fda.gov/cdrh_docs/reviews/k140407.pdf. Accessed November 1, 2014.

      5. PathoFinder’s GastroFinder Smart 17 Fast. 2013. Available at: http://www.pathofinder.com/products/smartfinder/gastrofinder-smart-17-fast. Accessed November 1, 2014.

      6. Verigene® Enteric Pathogens Nucleic Acid Test (EP) K140083. 2014. Available at: http://www.accessdata.fda.gov/cdrh_docs/pdf14/K140083.pdf. Accessed November 1, 2014.

      7. BD MAX Enteric Bacterial Panel K140111. 2014. Available at: http://www.accessdata.fda.gov/cdrh_docs/pdf14/K140111.pdf. Accessed November 1, 2014.

      8. Hologic (Gen-Probe) ProGastro SSCS Assay K123274. 2013. Available at: http://www.accessdata.fda.gov/cdrh_docs/pdf12/K123274.pdf. Accessed November 1, 2014.

        • Buchan B.W.
        • Olson W.J.
        • Pezewski M.
        • et al.
        Clinical evaluation of a real-time PCR assay for identification of Salmonella, Shigella, Campylobacter (Campylobacter jejuni and C. coli), and Shiga toxin-producing Escherichia coli isolates in stool specimens.
        J Clin Microbiol. 2013; 51: 4001-4007
      9. Serosep. Available at: http://www.entericbio.com. Accessed November 1, 2014.

        • Koziel M.
        • Kiely R.
        • Blake L.
        • et al.
        Improved detection of bacterial pathogens in patients presenting with gastroenteritis by use of the EntericBio real-time Gastro Panel I assay.
        J Clin Microbiol. 2013; 51: 2679-2685
      10. Genetic Signature. Available at: http://geneticsignatures.com/infectious-pathogen-detection/easyscreen-3base-technology/. Accessed November 1, 2014.

        • Siah S.P.
        • Merif J.
        • Kaur K.
        • et al.
        Improved detection of gastrointestinal pathogens using generalised sample processing and amplification panels.
        Pathology. 2014; 46: 53-59
        • Stark D.
        • Roberts T.
        • Ellis J.T.
        • et al.
        Evaluation of the EasyScreen™ enteric parasite detection kit for the detection of Blastocystis spp., Cryptosporidium spp., Dientamoeba fragilis, Entamoeba complex, and Giardia intestinalis from clinical stool samples.
        Diagn Microbiol Infect Dis. 2014; 78: 149-152
      11. R-Biopharma Web site. Available at: http://www.r-biopharm.com/products/clinical-diagnostics/molecular-diagnostics. Accessed November 1, 2014.

      12. Fast-track Diagnostics. Available at: http://www.fast-trackdiagnostics.com/products/. Accessed November 1, 2014.

      13. Diagenode Diagnostics. Available at: http://www.diagenodediagnostics.com/en/list-products-2.php. Accessed November 1, 2014.

        • Biswas J.S.
        • Al-Ali A.
        • Rajput P.
        • et al.
        A parallel diagnostic accuracy study of three molecular panels for the detection of bacterial gastroenteritis.
        Eur J Clin Microbiol Infect Dis. 2014; 33: 2075-2081
      14. Genomica. Available at: http://www.genomica.es/en/in_vitro_diagnostics_clart_enterobac.cfm. Accessed November 1, 2014.

      15. AusDiagnostics. Available at: http://www.ausdiagnostics.com/qilan/ADMainGeneList.jsp?ProductID=1142&CatNo=6509001. Accessed November 1, 2014.

        • Stark D.
        • Al-Qassab S.E.
        • Barratt J.L.
        • et al.
        Cryptosporidium spp., Dientamoeba fragilis, Entamoeba histolytica, and Giardia intestinalis in clinical stool samples.
        J Clin Microbiol. 2011; 49: 257-262
      16. Seegene. Available at: http://www.seegene.com/neo/en/introduction/core_dpo.php. Accessed November 1, 2014.

        • Coupland L.J.
        • McElarney I.
        • Meader E.
        • et al.
        Simultaneous detection of viral and bacterial enteric pathogens using the Seeplex® Diarrhea ACE detection system.
        Epidemiol Infect. 2013; 141: 2111-2121
        • Onori M.
        • Coltella L.
        • Mancinelli L.
        • et al.
        Evaluation of a multiplex PCR assay for simultaneous detection of bacterial and viral enteropathogens in stool samples of paediatric patients.
        Diagn Microbiol Infect Dis. 2014; 79: 149-154
        • Mengelle C.
        • Mansuy J.
        • Prere M.
        • et al.
        Simultaneous detection of gastrointestinal pathogens with a multiplex Luminex-based molecular assay in stool samples from diarrhoeic patients.
        Clin Microbiol Infect. 2013; 19: E458-E465
        • Patel A.
        • Navidad J.
        • Bhattacharyya S.
        Site-specific clinical evaluation of the Luminex xTAG gastrointestinal pathogen panel for detection of infectious gastroenteritis in fecal specimens.
        J Clin Microbiol. 2014; 52: 3068-3071
        • Zboromyrska Y.
        • Hurtado J.
        • Salvador P.
        • et al.
        Aetiology of traveller's diarrhoea: evaluation of a multiplex PCR tool to detect different enteropathogens.
        Clin Microbiol Infect. 2014; 20: O753-O759
        • Beckmann C.
        • Heininger U.
        • Marti H.
        • et al.
        Gastrointestinal pathogens detected by multiplex nucleic acid amplification testing in stools of pediatric patients and patients returning from the tropics.
        Infection. 2014; 42: 961-970
        • Malecki M.
        • Schildgen V.
        • Kamm M.
        • et al.
        Rapid screening method for multiple gastroenteric pathogens also detects novel enterohemorrhagic Escherichia coli O104:H4.
        Am J Infect Control. 2012; 40: 82-83
        • Claas E.C.
        • Burnham C.A.
        • Mazzulli T.
        • et al.
        Performance of the xTAG® gastrointestinal pathogen panel, a multiplex molecular assay for simultaneous detection of bacterial, viral, and parasitic causes of infectious gastroenteritis.
        J Microbiol Biotechnol. 2013; 23: 1041-1045
        • Halligan E.
        • Edgeworth J.
        • Bisnauthsing K.
        • et al.
        Multiplex molecular testing for management of infectious gastroenteritis in a hospital setting: a comparative diagnostic and clinical utility study.
        Clin Microbiol Infect. 2014; 20: O460-O467
        • Khare R.
        • Espy M.J.
        • Cebelinski E.
        • et al.
        Comparative evaluation of two commercial multiplex panels for detection of gastrointestinal pathogens by use of clinical stool specimens.
        J Clin Microbiol. 2014; 52: 3667-3673
        • Kahlau P.
        • Malecki M.
        • Schildgen V.
        • et al.
        Utility of two novel multiplexing assays for the detection of gastrointestinal pathogens - a first experience.
        Springerplus. 2013; 2: 106