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Review Article| Volume 33, ISSUE 4, P753-772, December 2013

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Molecular Pathology Techniques

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      References

        • Saiki R.K.
        • Gelfand D.H.
        • Stoffel S.
        • et al.
        Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
        Science. 1988; 239: 487-491
        • Myers T.W.
        • Gelfand D.H.
        Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase.
        Biochemistry. 1991; 30: 7661-7666
        • Heid C.A.
        • Stevens J.
        • Livak K.J.
        • et al.
        Real time quantitativePCR.
        Genome Res. 1996; 6: 986-994
        • VanGuilder H.D.
        • Vrana K.E.
        • Freeman W.M.
        Twenty-five years of quantitative PCR for gene expression analysis.
        Biotechniques. 2008; 44: 619-626
        • Spackman E.
        • Suarez D.L.
        Type A influenza virus detection and quantitation by real-time RT-PCR.
        Methods Mol Biol. 2008; 436: 19-26
        • Chamberlain J.S.
        • Gibbs R.A.
        • Ranier J.E.
        • et al.
        Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification.
        Nucleic Acids Res. 1988; 16: 11141-11156
        • Merante F.
        • Yaghoubian S.
        • Janeczko R.
        Principles of the xTAG respiratory viral panel assay.
        J Clin Virol. 2007; 40: S31-S35
        • Walker G.T.
        • Little M.
        • Nadeau J.
        • et al.
        Strand displacement amplification—an isothermal, in vitro DNA amplification technique.
        Nucleic Acids Res. 1992; 20: 1691-1696
        • Walker G.T.
        • Little M.C.
        • Nadeau J.G.
        • et al.
        Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system.
        Proc Natl Acad Sci U S A. 1992; 89: 392-396
        • Walker G.T.
        Empirical aspects of strand displacement amplification.
        PCR Methods Appl. 1993; 3 ([review]): 1-6
        • Down J.A.
        • O'Connell M.A.
        • Dey M.S.
        • et al.
        Detection of Mycobacterium tuberculosis in respiratory specimens by strand displacement amplification of DNA.
        J Clin Microbiol. 1996; 34: 860-865
      1. Available at: http://www.gen-probe.com/science/#technologies-0.

        • Ronaghi M.
        • Uhlén M.
        • Nyrén P.
        A sequencing method based on real-time pyrophosphate.
        Science. 1998; 281: 363
        • Nyrén P.
        The history of pyrosequencing.
        Methods Mol Biol. 2007; 373: 1-14
        • Nordstrom T.
        • Ronaghi M.
        • Forsberg L.
        • et al.
        Direct analysis of single-nucleotide polymorphism on double-stranded DNA by pyrosequencing.
        Biotechnol Appl Biochem. 2000; 31: 107-112
        • Fischer S.G.
        • Lerman L.S.
        DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.
        Proc Natl Acad Sci U S A. 1983; 80: 1579-1583
      2. Available at: http://www.ingeny.com/htdocs/DGGE.html.

        • McAuliffe L.
        • Ellis Richard J.
        • Lawes Jo R.
        • et al.
        16S rDNA PCR and denaturing gradient gel electrophoresis; a single generic test for detecting and differentiating Mycoplasma species.
        J Med Microbiol. 2005; 54: 731-739
        • van der Hout Annemarie H.
        • van den Ouweland Ans M.W.
        • van der Luijt Rob B.
        • et al.
        A DGGE system for comprehensive mutation screening of BRCA1 and BRCA2: application in a Dutch cancer clinic setting.
        Hum Mutat. 2006; 27: 654-666
        • Ririe K.
        • Rasmussen R.P.
        • Wittwer C.T.
        Product differentiation by analysis of DNA melting curves during the polymerase chain reaction.
        Anal Biochem. 1997; 245: 154-160
      3. Available at: http://www.kapabiosystems.com/public/pdfs/kapa-hrm-fast-pcrkits/Introduction_to_High_Resolution_Melt _Analysis_Guide.pdf.

        • Alberts B.
        • Johnson A.
        • Lewis J.
        • et al.
        Molecular biology of the cell.
        5th edition. Garland Science, Taylor & Francis Group, New York2008: 538-539
        • Southern E.M.
        Detection of specific sequences among DNA fragments separated by gel electrophoresis.
        J Mol Biol. 1975; 98: 503-517
        • Alwine J.C.
        • Kemp D.J.
        • Stark G.R.
        Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
        Proc Natl Acad Sci U S A. 1977; 74: 5350-5354
        • Kevil C.G.
        • Walsh L.
        • Laroux F.S.
        • et al.
        An improved, rapid northern protocol.
        Biochem Biophys Res Commun. 1997; 238: 277-279
        • Rosetti S.
        • Englisch S.
        • Bresin E.
        • et al.
        Detection of mutations in human genes by a new rapid method: cleavage fragment length polymorphism analysis (CFLPA).
        Mol Cell Probes. 1997; 11: 155-160
        • Molhuizen H.O.
        • Bunschoten A.E.
        • Schouls L.M.
        • et al.
        Rapid detection and simultaneous strain differentiation of Mycobacterium tuberculosis complex bacteria by spoligotyping.
        Methods Mol Biol. 1998; 101: 381-394
        • Cowan L.S.
        • Diem L.
        • Brake M.C.
        • et al.
        Transfer of a Mycobacterium tuberculosis genotyping method, spoligotyping, from a reverse line-blot hybridization, membrane-based assay to the luminex multianalyte profiling system.
        J Clin Microbiol. 2004; 42: 474-477
        • Poljak M.
        • Brenčič A.
        • Seme K.
        • et al.
        Comparative evaluation of first- and second-generation digene hybrid capture assays for detection of human papillomaviruses associated with high or intermediate risk for cervical cancer.
        J Clin Microbiol. 1999; 37: 796-797
      4. Available at: http://www.thehpvtest.com/∼/media/5C4BD0982BED4E3788F65B36AF829AAD.ashx.

        • Collins M.L.
        • Zayati C.
        • Detmer J.J.
        • et al.
        Preparation and characterization of RNA standards for use in quantitative branched-DNA hybridization assays.
        Anal Biochem. 1995; 226: 120-129
        • Gall J.G.
        • Pardue M.L.
        Formation and detection of RNA-DNA hybrid molecules in cytological preparations.
        Proc Natl Acad Sci U S A. 1969; 63: 378-383
        • Tautz D.
        • Pfeifle C.
        A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback.
        Chromosoma. 1989; 98: 81-85
        • Jin L.
        • Lloyd R.V.
        In situ hybridization: methods and applications.
        J Clin Lab Anal. 1997; 11: 2-9
        • Tubbs R.
        • Pettay J.
        • Skacel M.
        • et al.
        Gold-facilitated in situ hybridization: a bright-field autometallographic alternative to fluorescence in situ hybridization for detection of HER-2/neu gene amplification.
        Am J Pathol. 2002; 160: 1589-1595
        • Bluth M.H.
        Hybridization array technologies in McPherson and Pincus: Henry’s clinical diagnosis and management by laboratory methods.
        22nd edition. Saunders Publishing, New York2011: 1282-1289