Advertisement
Review Article| Volume 33, ISSUE 3, P589-609, September 2013

MALDI-TOF Mass Spectrometry for Microorganism Identification

      Keywords

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribers receive full online access to your subscription and archive of back issues up to and including 2002.

      Content published before 2002 is available via pay-per-view purchase only.

      Subscribe:

      Subscribe to Clinics in Laboratory Medicine
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Gaieski D.F.
        • Pines J.M.
        • Band R.A.
        • et al.
        Impact of time to antibiotics on survival in patients with severe sepsis or septic shock in whom early goal-directed therapy was initiated in the emergency department.
        Crit Care Med. 2010; 38: 1045-1053
        • Kumar A.
        • Roberts D.
        • Wood K.E.
        • et al.
        Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock.
        Crit Care Med. 2006; 34: 1589-1596
        • Bizzini A.
        • Greub G.
        Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification.
        Clin Microbiol Infect. 2010; 16: 1614-1619
        • Carbonnelle E.
        • Mesquita C.
        • Bille E.
        • et al.
        MALDI-TOF mass spectrometry for bacterial identification in the clinical microbiology laboratory.
        Clin Biochem. 2011; 44: 104-109
        • Seng P.
        • Drancourt M.
        • Gouriet F.
        • et al.
        Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
        Clin Infect Dis. 2009; 49: 543-551
        • Anhalt J.P.
        • Fenselau C.
        Identification of bacteria using mass spectrometry.
        Anal Chem. 1975; 47: 219-225
        • Hillenkamp F.
        • Karas M.
        Mass spectrometry of peptides and proteins by matrix-assisted ultraviolet laser desorption/ionization.
        Methods Enzymol. 1990; 193: 280-295
        • Karas M.
        • Bachmann D.
        • Hillenkamp F.
        Influence of the wavelength in high-irradiance ultraviolet laser desorption mass spectrometry of organic molecules.
        Anal Chem. 1985; 57: 2935-2939
        • Karas M.
        • Bachmann D.
        • Bahr U.
        • et al.
        Matrix-assisted ultraviolet laser desorption of non-volatile compounds.
        Int J Mass Spectrom Ion Process. 1987; 78: 53-68
        • Karas M.
        • Hillenkamp F.
        Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons.
        Anal Chem. 1988; 60: 2299-2301
        • Claydon M.A.
        • Davey S.N.
        • Edwards-Jones V.
        • et al.
        The rapid identification of intact microorganisms using mass spectrometry.
        Nat Biotechnol. 1996; 14: 1584-1586
        • Holland R.D.
        • Wilkes J.G.
        • Rafii F.
        • et al.
        Rapid identification of intact whole bacteria based on spectral patterns using matrix-assisted laser desorption/ionization with time of flight mass spectrometry.
        Rapid Commun Mass Spectrom. 1996; 10: 1227-1232
        • Krishnamurthy T.
        • Ross P.L.
        Rapid identification of bacteria by direct matrix-assisted laser desorption/ionization mass spectrometric analysis of whole cells.
        Rapid Commun Mass Spectrom. 1996; 10: 1992-1996
        • Welham K.J.
        • Domin M.A.
        • Scannell D.E.
        • et al.
        The characterization of micro-organisms by matrix-assisted laser desorption time-of-flight mass spectrometry.
        Rapid Commun Mass Spectrom. 1998; 12: 176-180
        • Emonet S.
        • Shah H.N.
        • Cherkaoui A.
        • et al.
        Application and use of various mass spectrometry methods in clinical microbiology.
        Clin Microbiol Infect. 2010; 16: 1604-1613
        • Fenselau C.
        • Demirev P.A.
        Characterization of intact microorganisms by MALDI mass spectrometry.
        Mass Spectrom Rev. 2001; 20: 157-171
        • Bizzini A.
        • Durussel C.
        • Bille J.
        • et al.
        Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory.
        J Clin Microbiol. 2010; 48: 1549-1554
        • Smole S.C.
        • King L.A.
        • Leopold P.E.
        • et al.
        Sample preparation of gram positive bacteria for identification by matrix assisted laser desorption/ionization time-of-flight.
        J Microbiol Methods. 2002; 48: 107-115
        • van Veen S.Q.
        • Claas E.C.
        • Kuijper E.J.
        High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories.
        J Clin Microbiol. 2010; 48: 900-907
        • Holland R.D.
        • Duffy C.R.
        • Rafii F.
        • et al.
        Identification of bacterial proteins observed in MALDI TOF mass spectra from whole cells.
        Anal Chem. 1999; 71: 3226-3230
        • Rhyzhov V.
        • Fenselau C.
        Characterization of the protein subset desorbed by MALDI from whole bacterial cells.
        Anal Chem. 2001; 73: 746-750
        • Sun L.
        • Teramoto K.
        • Sato H.
        • et al.
        Characterization of ribosomal proteins as biomarkers for matrix-assisted laser desorption/ionization mass spectral identification of Lactobacillus plantarum.
        Rapid Commun Mass Spectrom. 2006; 20: 3789-3798
        • Sogawa K.
        • Watanabe M.
        • Sato K.
        • et al.
        Rapid identification of microorganisms by mass spectrometry: improved performance by incorporation of in-house spectral data into a commercial database.
        Anal Bioanal Chem. 2012; 403: 1811-1822
        • Saenz A.J.
        • Petersen C.E.
        • Valentine N.B.
        • et al.
        Reproducibility of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for replicate bacterial culture analysis.
        Rapid Commun Mass Spectrom. 1999; 13: 1580-1585
        • Wieser A.
        • Schneider L.
        • Jung J.
        • et al.
        MALDI-TOF MS in microbiological diagnostics-identification of microorganisms and beyond (mini-review).
        Appl Microbiol Biotechnol. 2012; 93: 965-974
        • Welker M.
        • Moore E.R.
        Applications of whole-cell matrix-assisted laser-desorption/ionization time of flight mass spectrometry in systematic microbiology.
        Syst Appl Microbiol. 2011; 34: 2-11
        • Sogawa K.
        • Watanabe M.
        • Sato K.
        • et al.
        Use of MALDI BioTyper system with MALDI-TOF mass spectrometry for rapid identification of microorganisms.
        Anal Bioanal Chem. 2011; 400: 1905-1911
        • Bille E.
        • Dauphin B.
        • Leto J.
        • et al.
        MALDI-TOF MS Andromas strategy for the routine identification of bacteria, mycobacteria, yeasts, Aspergillus spp. and positive blood cultures.
        Clin Microbiol Infect. 2012; 18: 1117-1125
        • Cherkaoui A.
        • Hibbs J.
        • Emonet S.
        • et al.
        Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level.
        J Clin Microbiol. 2010; 48: 1169-1175
        • Carbonnelle E.
        • Grohs P.
        • Jacquier H.
        • et al.
        Robustness of two MALDI-TOF mass spectrometry systems for bacterial identification.
        J Microbiol Methods. 2012; 89: 133-136
        • Dubois D.
        • Grare M.
        • Prere M.F.
        • et al.
        Performances of the VITEK MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid identification of bacteria in routine clinical microbiology.
        J Clin Microbiol. 2012; 50: 2568-2576
        • Farfour E.
        • Leto J.
        • Barritault M.
        • et al.
        Evaluation of the Andromas matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of aerobically growing gram-positive bacilli.
        J Clin Microbiol. 2012; 50: 2702-2707
        • Fernandex-Olmos A.
        • Garcia-Castillo M.
        • Morosini M.I.
        • et al.
        MALDI-TOF MS improves routine identification of non-fermenting gram negative isolates from cystic fibrosis patients.
        J Cyst Fibros. 2012; 11: 59-62
        • Marko D.C.
        • Saffert R.T.
        • Cunningham S.A.
        • et al.
        Evaluation of the Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of nonfermenting gram-negative bacilli isolated from culture from cystic fibrosis patients.
        J Clin Microbiol. 2012; 50: 2034-2039
        • Saleeb P.G.
        • Drake S.K.
        • Murray P.R.
        • et al.
        Identification of mycobacteria in solid-culture media by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
        J Clin Microbiol. 2011; 49: 1790-1794
        • Veloo A.C.
        • Knoester M.
        • Degener J.E.
        • et al.
        Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods for the identification of clinically relevant anaerobic bacteria.
        Clin Microbiol Infect. 2011; 17: 1501-1506
        • Fedorko D.P.
        • Drake S.K.
        • Stock F.
        • et al.
        Identification of clinical isolates of anaerobic bacteria using matrix-assisted laser desorption ionization-time of flight mass spectrometry.
        Eur J Clin Microbiol Infect Dis. 2012; 31: 2257-2262
        • Fournier R.
        • Wallet F.
        • Grandbastien B.
        • et al.
        Chemical extraction versus direct smear for MALDI-TOF mass spectrometry identification of anaerobic bacteria.
        Anaerobe. 2012; 18: 294-297
        • Nagy E.
        • Becker S.
        • Kostrzewa M.
        • et al.
        The value of MALDI-TOF MS for the identification of clinically relevant anaerobic bacteria in routine laboratories.
        J Med Microbiol. 2012; 61: 1393-1400
        • Van Herendael B.H.
        • Bruynseels P.
        • Bensaid M.
        • et al.
        Validation of a modified algorithm for the identification of yeast isolates using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
        Eur J Clin Microbiol Infect Dis. 2012; 31: 841-848
        • Dhiman N.
        • Hall L.
        • Wohlfiel S.L.
        • et al.
        Performance and cost analysis of matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine identification of yeast.
        J Clin Microbiol. 2011; 49: 1614-1616
        • Bader O.
        • Weig M.
        • Taverne-Ghadwal L.
        • et al.
        Improved clinical laboratory identification of human pathogenic yeasts by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
        Clin Microbiol Infect. 2011; 17: 1359-1365
        • Alanio A.
        • Beretti J.L.
        • Dauphin B.
        • et al.
        Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for fast and accurate identification of clinically relevant Aspergillus species.
        Clin Microbiol Infect. 2011; 17: 750-755
        • De Carolis E.
        • Posteraro B.
        • Lass-Flori C.
        • et al.
        Species identification of Aspergillus, Fusarium and Mucorales with direct surface analysis by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
        Clin Microbiol Infect. 2012; 18: 475-484
        • Alshawa K.
        • Beretti J.L.
        • Lacroix C.
        • et al.
        Successful identification of clinical dermatophyte and Neoscytalidium species by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
        J Clin Microbiol. 2012; 50: 2277-2281
        • Tan K.E.
        • Ellis C.
        • Lee R.
        • et al.
        Prospective evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry system in a hospital clinical microbiology laboratory for identification of bacteria and yeasts: a bench-by-bench study for assessing the impact on time to identification and cost-effectiveness.
        J Clin Microbiol. 2012; 50: 3301-3308
        • Gaillot O.
        • Blondiaux N.
        • Loiez C.
        • et al.
        Cost-effectiveness of switch to matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine bacterial identification.
        J Clin Microbiol. 2011; 49: 4412
        • Ikryannikova L.N.
        • Filimonova A.V.
        • Malakhova M.V.
        • et al.
        Discrimination between Streptococcus pneumoniae and Streptococcus mitis based on sorting of their MALDI mass spectra.
        Clin Microbiol Infect. 2013; ([Epub ahead of print])
        • Christner M.
        • Rohde H.
        • Wolters M.
        • et al.
        Rapid identification of bacteria from positive blood culture bottles by use of matrix-assisted laser desorption-ionization time of flight mass spectrometry fingerprinting.
        J Clin Microbiol. 2010; 48: 1584-1591
        • Buchan B.W.
        • Riebe K.M.
        • Ledeboer N.A.
        Comparison of the MALDI Biotyper system using Sepsityper specimen processing to routine microbiological methods for identification of bacteria from positive blood culture bottles.
        J Clin Microbiol. 2012; 50: 346-352
        • Ferroni A.
        • Suarez S.
        • Beretti J.L.
        • et al.
        Real-time identification of bacteria and Candida species in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
        J Clin Microbiol. 2010; 48: 1542-1548
        • Klein S.
        • Zimmermann S.
        • Kohler C.
        • et al.
        Integration of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in blood culture diagnostics: a fast and effective approach.
        J Med Microbiol. 2012; 61: 323-331
        • Kok J.
        • Thomas L.C.
        • Olma T.
        • et al.
        Identification of bacteria in blood culture broths using matrix-assisted laser desorption-ionization Sepsityper and time of flight mass spectrometry.
        PLoS One. 2011; 6: e23285
        • Lagace-Wiens P.R.
        • Adam H.J.
        • Karlowsky J.A.
        • et al.
        Identification of blood culture isolates directly from positive blood cultures by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and a commercial extraction system: analysis of performance, cost and turnaround time.
        J Clin Microbiol. 2012; 50: 3324-3328
        • Loonen A.J.
        • Jansz A.R.
        • Stalpers J.
        • et al.
        An evaluation of three processing methods and the effect of reduced culture times for faster direct identification of pathogens from BacT/ALERT blood cultures by MALDI-TOF MS.
        Eur J Clin Microbiol Infect Dis. 2012; 31: 1575-1583
        • Martiny D.
        • Dediste A.
        • Vandenberg O.
        Comparison of an in-house method and the commercial Sepsityper kit for bacterial identification directly from positive blood culture broths by matrix-assisted laser desorption-ionization time of flight mass spectrometry.
        Eur J Clin Microbiol Infect Dis. 2012; 31: 2269-2281
        • Meex C.
        • Neuville F.
        • Descy J.
        • et al.
        Direct identification of bacteria from the BacT/ALERT anaerobic blood cultures by MALDI-TOF MS: MALDI Sepsityper kit versus an in-house method for bacterial extraction.
        J Med Microbiol. 2012; 61: 1511-1516
        • Prod'hom G.
        • Bizzini A.
        • Durussel C.
        • et al.
        Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets.
        J Clin Microbiol. 2010; 48: 1481-1483
        • Stevenson L.G.
        • Drake S.K.
        • Murray P.R.
        Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
        J Clin Microbiol. 2010; 48: 444-447
        • Yan Y.
        • He Y.
        • Maier T.
        • et al.
        Improved identification of yeast species directly from positive blood culture media by combining Sepsityper specimen procession and Microflex analysis with the matrix-assisted laser desorption ionization Biotyper system.
        J Clin Microbiol. 2011; 49: 2528-2532
        • La Scola B.
        • Raoult D.
        Direct identification of positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry.
        PLoS One. 2009; 4: e8041
        • Vlek A.L.
        • Bonten M.J.
        • Boel C.H.
        Direct matrix-assisted laser desportion ionization time-of-flight mass spectrometry improves appropriateness of antibiotic treatment of bacteremia.
        PLoS One. 2012; 7: e32589
        • Clerc O.
        • Prod'hom G.
        • Vogne C.
        • et al.
        Impact of matrix-assisted laser desorption ionization time-of-flight mass spectrometry on the clinical management of patients with Gram-negative bacteremia: a prospective observational study.
        Clin Infect Dis. 2013; 56: 1101-1107
        • Kohling H.L.
        • Bittner A.
        • Muller K.D.
        • et al.
        Direct identification of bacteria in urine samples by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and relevance of defensins as interfering factors.
        J Med Microbiol. 2012; 61: 339-344
        • Ferreira L.
        • Sanchez-Juanes F.
        • Gonzalez-Avila M.
        • et al.
        Direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
        J Clin Microbiol. 2010; 48: 2110-2115
        • Klevens R.M.
        • Edwards J.R.
        • Richards Jr., C.L.
        • et al.
        Estimating health care-associated infections and deaths in U.S. hospitals, 2002.
        Public Health Rep. 2007; 122: 160-166
        • Bernardo K.
        • Pakulat N.
        • Macht M.
        • et al.
        Identification and discrimination of Staphylococcus aureus strains using matrix-assisted laser desorption/ionization-time of flight mass spectrometry.
        Proteomics. 2002; 2: 747-753
        • Du Z.
        • Yang R.
        • Guo Z.
        • et al.
        Identification of Staphylococcus aureus and determination of its methicillin resistance by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
        Anal Chem. 2002; 74: 5487-5491
        • Edwards-Jones V.
        • Claydon M.A.
        • Evason D.J.
        • et al.
        Rapid discrimination between methicillin-sensitive and methicillin-resistant Staphylococcus aureus by intact cell mass spectrometry.
        J Med Microbiol. 2000; 49: 295-300
        • Lu J.J.
        • Tsai F.J.
        • Ho C.M.
        • et al.
        Peptide biomarker discovery for identification of methicillin-resistant and vancomycin-intermediate Staphylococcus aureus strains by MALDI-TOF.
        Anal Chem. 2012; 84: 5685-5692
        • Griffin P.M.
        • Price G.R.
        • Schooneveldt J.M.
        • et al.
        Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry to identify vancomycin-resistant enterococci and investigate the epidemiology of an outbreak.
        J Clin Microbiol. 2012; 50: 2918-2931
        • Schaumann R.
        • Knoop N.
        • Genzel G.H.
        • et al.
        A step towards the discrimination of beta-lactamase-producing clinical isolates of Enterobacteriaceae and Pseudomonas aeruginosa by MALDI-TOF mass spectrometry.
        Med Sci Monit. 2012; 18: MT71-MT77
        • Sparbier K.
        • Schubert S.
        • Weller U.
        • et al.
        Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based functional assay for rapid detection of resistance against B-lactam antibiotics.
        J Clin Microbiol. 2012; 50: 927-937
        • Burckhardt I.
        • Zimmermann S.
        Using matrix-assisted laser desorption ionization-time of flight mass spectrometry to detect carbapenem resistance within 1 to 2.5 hours.
        J Clin Microbiol. 2011; 49: 3321-3324
        • Hrabak J.
        • Walkova R.
        • Studentova V.
        • et al.
        Carbapenemase activity detection by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
        J Clin Microbiol. 2011; 49: 3222-3227
        • Hrabak J.
        • Studentova V.
        • Walkova R.
        • et al.
        Detection of NDM-1, VIM-1, KPC, OXA-48, and OXA-162 carbapenemases by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
        J Clin Microbiol. 2012; 50: 2441-2443
        • Kempf M.
        • Bakour S.
        • Flaudrops C.
        • et al.
        Rapid detection of carbapenem resistance in Acinetobacter baumannii using matrix-assisted laser desorption ionization-time of flight mass spectrometry.
        PLoS One. 2012; 7: e31676
        • Murray P.R.
        Matrix-assisted laser desorption ionization time-of-flight mass spectrometry: usefulness for taxonomy and epidemiology.
        Clin Microbiol Infect. 2010; 16: 1626-1630
        • Wolters M.
        • Rohde H.
        • Maier T.
        • et al.
        MALDI-TOF MS fingerprinting allows for discrimination of major methicillin-resistant Staphylococcus aureus lineages.
        Int J Med Microbiol. 2011; 301: 64-68
        • Jackson K.A.
        • Edwards-Jones V.
        • Sutton C.W.
        • et al.
        Optimisation of intact cell MALDI method for fingerprinting of methicillin Staphylococcus aureus.
        J Microbiol Methods. 2005; 62: 273-284
        • Walker J.
        • Fox A.J.
        • Edwards-Jones V.
        • et al.
        Intact cell mass spectrometry (ICMS) used to type methicillin-resistant Staphylococcus aureus: media effects and inter-laboratory reproducibility.
        J Microbiol Methods. 2002; 48: 117-126
        • Barbuddhe S.B.
        • Maier T.
        • Schwarz G.
        • et al.
        Rapid identification and typing of Listeria species by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
        Appl Environ Microbiol. 2008; 74: 5402-5407
        • Williamson Y.M.
        • Moura H.
        • Woolfitt A.R.
        • et al.
        Differentiation of Streptococcus pneumoniae conjunctivitis outbreak isolates by matrix-assisted laser desorption-time of flight mass spectrometry.
        Appl Environ Microbiol. 2008; 74: 5891-5897
        • Dieckmann R.
        • Helmuth R.
        • Erhard M.
        • et al.
        Rapid classification and identification of salmonellae at the species and subspecies levels by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry.
        Appl Environ Microbiol. 2008; 74: 7767-7778
        • Marinach C.
        • Alanio A.
        • Palous M.
        • et al.
        MALDI-TOF MS-based drug susceptibility testing of pathogens: the example of Candida albicans and fluconazole.
        Proteomics. 2009; 9: 4627-4631
        • Stephan R.
        • Cernela N.
        • Ziegler D.
        • et al.
        Rapid species specific identification and subtyping of Yersinia enterocolitica by MALDI-TOF mass spectrometry.
        J Microbiol Methods. 2011; 87: 150-153
        • Justesen U.S.
        • Holm A.
        • Knudsen E.
        • et al.
        Species identification of clinical isolates of anaerobic bacteria: a comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems.
        J Clin Microbiol. 2011; 49: 4314-4318
        • Marklein G.
        • Josten M.
        • Klanke U.
        • et al.
        Matrix-assisted laser desorption-time of flight mass spectrometry for fast and reliable identification of clinical yeast isolates.
        J Clin Microbiol. 2009; 47: 2912-2917
        • Stevenson L.G.
        • Drake S.K.
        • Shea Y.R.
        • et al.
        Evaluation of matrix assisted laser deorption ionization-time of flight mass spectrometry for identification of clinically important yeast species.
        J Clin Microbiol. 2010; 48: 3482-3486